公开(公告)号：US20140234258A1

公开(公告)日：2014-08-21

申请号：US14226990

申请日：2014-03-27

申请人： University of Medicine and Dentistry of New Jersey

发明人： Masayori Inouye ,  Junjie Zhang ,  Yong Long Zhang

IPC分类号： C12N9/22

CPC分类号： C12N9/22 ,  A61K38/465 ,  C12N15/70 ,  C12P19/34 ,  C12Q1/42 ,  C12Q1/44 ,  G01N2333/922 ,  G01N2800/26

摘要： The present invention is directed to the discovery of a novel family of enzymes designated herein as mRNA interferases that exhibit endoribonuclease activity. The novel finding of the present inventors, therefore, presents new applications for which mRNA interferase nucleic and amino acid sequences, and compositions thereof may be used to advantage. The invention also encompasses screening methods to identify compounds/agents capable of modulating mRNA interferase activity and methods for using such compounds/agents. Also provided is a kit comprising mRNA interferase nucleic and/or amino acid sequences, mRNA interferase activity compatible buffers, and instruction materials.

摘要翻译： 本发明涉及在本文中称为表达内切核糖核酸酶活性的mRNA干扰酶的新型家族的发现。 因此，本发明人的新发现提出了可以使用mRNA干扰酶核酸和氨基酸序列及其组合物的新用途。 本发明还包括鉴定能够调节mRNA干扰酶活性的化合物/试剂的筛选方法以及使用这些化合物/试剂的方法。 还提供了包含mRNA干扰酶核酸和/或氨基酸序列，mRNA干扰酶活性相容缓冲液和说明材料的试剂盒。

公开(公告)号：US20140193878A1

公开(公告)日：2014-07-10

申请号：US13932498

申请日：2013-07-01

申请人： University of Medicine and Dentistry of New Jersey

发明人： Masayori Inouye ,  Hirofumi Nariya

IPC分类号： C12N9/16

CPC分类号： C12N9/16 ,  C07K14/195

摘要： A regulated deployment of a toxin gene for developmental programmed cell death in bacteria is described. M. xanthus is demonstrated to have a solitary mazF gene that lacks a cotranscribed antitoxin gene. Deletion of mazF results in elimination of the obligatory cell death during development causing dramatic reduction in spore formation. Surprisingly, MrpC functions as a MazF antitoxin and a mazF transcription activator. Transcription of mrpC and mazF is negatively regulated via MrpC phosphorylation by a Ser/Thr kinase cascade. Various methods of exploiting this novel pathway are described herein.

摘要翻译： 描述了在细菌中发育程序性细胞死亡的毒素基因的调节性部署。 黄曲霉被证明具有缺乏共转录的抗毒素基因的孤立的mazF基因。 mazF的删除导致在发育过程中消除强制性细胞死亡，导致孢子形成的显着减少。 令人惊讶的是，MrpC起MazF抗毒素和mazF转录激活物的作用。 mrpC和mazF的转录通过Ser / Thr激酶级联的MrpC磷酸化负调控。 本文描述了利用这种新途径的各种方法。