公开(公告)号：US20140193878A1

公开(公告)日：2014-07-10

申请号：US13932498

申请日：2013-07-01

申请人： University of Medicine and Dentistry of New Jersey

发明人： Masayori Inouye ,  Hirofumi Nariya

IPC分类号： C12N9/16

CPC分类号： C12N9/16 ,  C07K14/195

摘要： A regulated deployment of a toxin gene for developmental programmed cell death in bacteria is described. M. xanthus is demonstrated to have a solitary mazF gene that lacks a cotranscribed antitoxin gene. Deletion of mazF results in elimination of the obligatory cell death during development causing dramatic reduction in spore formation. Surprisingly, MrpC functions as a MazF antitoxin and a mazF transcription activator. Transcription of mrpC and mazF is negatively regulated via MrpC phosphorylation by a Ser/Thr kinase cascade. Various methods of exploiting this novel pathway are described herein.

摘要翻译： 描述了在细菌中发育程序性细胞死亡的毒素基因的调节性部署。 黄曲霉被证明具有缺乏共转录的抗毒素基因的孤立的mazF基因。 mazF的删除导致在发育过程中消除强制性细胞死亡，导致孢子形成的显着减少。 令人惊讶的是，MrpC起MazF抗毒素和mazF转录激活物的作用。 mrpC和mazF的转录通过Ser / Thr激酶级联的MrpC磷酸化负调控。 本文描述了利用这种新途径的各种方法。