公开(公告)号：US20170240901A1

公开(公告)日：2017-08-24

申请号：US15121742

申请日：2015-02-26

Applicant: The University of Liverpool

Inventor： John Hunt ,  John Innes

IPC: C12N15/113 ,  A61K31/713 ,  A61K9/50

CPC classification number: C12N15/1136 ,  A61K9/5031 ,  A61K31/713 ,  C12N2310/14 ,  C12N2320/30 ,  C12N2320/32

Abstract: Provided are siRNA molecules that are particularly efficient in their ability to reduce transcription and expression of TNF or IL-1β in canine macrophage and synovial cells. The siRNA molecules demonstrate improved silencing of TNF or IL-1β as compared to siRNA molecules disclosed prior to the present invention. These properties are demonstrated in accepted experimental models of osteoarthritis, and particularly canine osteoarthritis. The siRNA molecules of the invention, may be employed in pharmaceutical compositions for use in the prevention and/or reduction of osteoarthritis in dogs. The inventors have found that encapsulation of the siRNAs in small PLGA microspheres confers surprisingly advantageous properties.

公开(公告)号：US20160166736A1

公开(公告)日：2016-06-16

申请号：US14908919

申请日：2014-07-29

Applicant: THE UNIVERSITY OF LIVERPOOL

Inventor： Judith Curran ,  John Hunt

IPC: A61L27/54 ,  C12N5/0775 ,  A61L27/10 ,  A61L27/18 ,  A61K31/131 ,  A61K31/695

CPC classification number: A61L27/54 ,  A61C8/0013 ,  A61K31/131 ,  A61K31/695 ,  A61L27/10 ,  A61L27/18 ,  A61L27/28 ,  A61L27/3821 ,  A61L27/3847 ,  A61L27/50 ,  A61L31/08 ,  A61L31/14 ,  A61L2300/414 ,  A61L2400/12 ,  A61L2400/18 ,  A61L2430/02 ,  A61L2430/12 ,  C12N5/0654 ,  C12N5/0662 ,  C12N2506/1346 ,  C12N2533/00 ,  C12N2533/12 ,  C12N2533/30 ,  C12N2533/40

Abstract: Provided is an osteoinductive material comprising a substrate; and a layer of a hydrocarbyl group comprising 10 to 12 carbon atoms, the hydrocarbyl group having one end attached to the substrate and a presenting amine (—NH2) group at the unattached end. The hydrocarbyl group may be 11 carbons long, and may be provided in the form of 11-aminoundecyltriethoxysilane. The substrate may be a glass; metal; polymer; ceramic; plastic; or hydroxyapatite. The substrate may, for example, be a metal selected from the group consisting of: titanium; titanium alloys; cobalt chrome; and steel. An osteoinductive material may be provided in the form of a medical implant, or part thereof, such as an orthopaedic implant or a dental implant or part thereof. Alternatively the substrate may be in the form of a cell culture vessel. Also provided is a method of producing such osteoinductive materials, the medical use of such materials, and a method of producing bone or a bone precursor utilising the disclosed materials.

Abstract translation: 提供了包含基底的骨诱导材料; 和包含10至12个碳原子的烃基基团，其中一个末端连接到基底上的烃基和未连接末端的呈现胺（-NH 2）基团。 烃基可以为11个碳原子，并且可以以11-氨基十九烷基三乙氧基硅烷的形式提供。 基底可以是玻璃; 金属; 聚合物; 陶瓷 塑料; 或羟基磷灰石。 基材可以是例如选自钛的金属; 钛合金; 钴铬; 和钢铁。 骨诱导材料可以以医疗植入物或其一部分的形式提供，例如矫形植入物或牙科植入物或其部分。 或者，底物可以是细胞培养容器的形式。 还提供了生产这种骨诱导材料的方法，这种材料的医学用途，以及利用所公开的材料制备骨骼或骨骼前体的方法。