公开(公告)号：US11053288B2

公开(公告)日：2021-07-06

申请号：US15424372

申请日：2017-02-03

Applicant: Trianni, Inc

Inventor： Nigel Killeen ,  Christoph Hasenhindl ,  Bao Duong

IPC: C07K14/315 ,  C07K16/00 ,  C07K14/705 ,  C07K14/73 ,  C07K14/74 ,  C12N5/0781 ,  C12N5/12 ,  C12N5/16 ,  A01K67/027 ,  C07K16/42

Abstract: The present invention provides methods and compositions for generating transgenic animals, including transgenic mammals, as well as plasma cells that allow for cell surface capture of secreted immunoglobulin molecules produced endogenously in the plasma cells.

公开(公告)号：US11889821B2

公开(公告)日：2024-02-06

申请号：US17030057

申请日：2020-09-23

Applicant: TRIANNI, INC.

Inventor： Matthias Wabl ,  Bao Duong ,  Werner Mueller

IPC: A01K67/027 ,  C07K16/00 ,  C12N15/85 ,  C12N5/12 ,  C12N15/11

CPC classification number: A01K67/0278 ,  C07K16/00 ,  C12N5/12 ,  C12N15/11 ,  C12N15/85 ,  A01K2217/072 ,  A01K2227/105 ,  A01K2267/01 ,  A01K2267/03 ,  C07K2317/52 ,  C07K2317/60

Abstract: The invention provides compositions and methods for enhanced production of immunoglobulin diversity. Specifically, the invention provides compositions and methods for making accessible a B cell receptor repertoire that has not been culled by developmental tolerance mechanisms. The invention also provides transgenic animals, cells, and antibodies resulting from these compositions and methods.

公开(公告)号：US10813346B2

公开(公告)日：2020-10-27

申请号：US15781025

申请日：2016-11-30

Applicant: Trianni, Inc

Inventor： Matthias Wahl ,  Bao Duong ,  Nigel Killeen

IPC: A01K67/027 ,  C07K16/00 ,  C12N15/85 ,  C12N5/12 ,  C12N15/11

Abstract: The invention provides compositions and methods for enhanced production of immunoglobulin diversity. Specifically, the invention provides compositions and methods for making accessible a B cell receptor repertoire that has not been culled by developmental tolerance mechanisms. The invention also provides transgenic animals, cells, and antibodies resulting from these compositions and methods.

公开(公告)号：US20170226162A1

公开(公告)日：2017-08-10

申请号：US15424372

申请日：2017-02-03

Applicant: Trianni, Inc

Inventor： Nigel Killeen ,  Christoph Hasenhindl ,  Bao Duong

IPC: C07K14/315 ,  C07K14/705 ,  C07K14/73 ,  C12N5/12 ,  C07K14/74 ,  A01K67/027 ,  C12N5/0781 ,  C07K16/00 ,  C07K16/42

Abstract: The present invention provides methods and compositions for generating transgenic animals, including transgenic mammals, as well as plasma cells that allow for cell surface capture of secreted immunoglobulin molecules produced endogenously in the plasma cells.

公开(公告)号：US11946063B2

公开(公告)日：2024-04-02

申请号：US16165018

申请日：2018-10-19

Applicant: TRIANNI, INC.

Inventor： Matthias Wabl ,  Werner Mueller ,  Peter Burrows ,  Gloria Esposito ,  Bao Duong

IPC: C12N15/85 ,  A01K67/027 ,  A01K67/0278 ,  C07K16/18 ,  C12N5/074 ,  C12N15/10 ,  C12N15/62 ,  C40B40/10

CPC classification number: C12N15/8509 ,  A01K67/0278 ,  C07K16/18 ,  C12N5/0696 ,  C12N15/1072 ,  C12N15/62 ,  C40B40/10 ,  A01K2207/15 ,  A01K2217/054 ,  A01K2217/072 ,  A01K2227/105 ,  A01K2267/01 ,  A01K2267/03 ,  C12N2015/8518 ,  C12N2015/8527

Abstract: The invention relates to a long DH (LDH) cassette comprising a recombinant DH construct comprising at least two DH gene segments encoding at least 10 amino acids of the HCDR3 amino acid sequence, wherein at least one of the DH gene segments is a heterologous DH gene segment; an immunoglobulin heavy chain locus and a transgenic non-human animal comprising the same; and their use in producing an immunoglobulin library with long HCDR3 regions.

公开(公告)号：US20190053474A1

公开(公告)日：2019-02-21

申请号：US15781025

申请日：2016-11-30

Applicant: Trianni, Inc

Inventor： Matthias Wabl ,  Bao Duong ,  Nigel Killeen

IPC: A01K67/027 ,  C12N15/11 ,  C12N5/12 ,  C12N15/85 ,  C07K16/00

CPC classification number: A01K67/0278 ,  A01K2217/072 ,  A01K2227/105 ,  A01K2267/01 ,  A01K2267/03 ,  C07K16/00 ,  C07K2317/52 ,  C07K2317/60 ,  C12N5/12 ,  C12N15/11 ,  C12N15/85

Abstract: The invention provides compositions and methods for enhanced production of immunoglobulin diversity. Specifically, the invention provides compositions and methods for making accessible a B cell receptor repertoire that has not been culled by developmental tolerance mechanisms. The invention also provides transgenic animals, cells, and antibodies resulting from these compositions and methods.

公开(公告)号：US20170058052A1

公开(公告)日：2017-03-02

申请号：US15246181

申请日：2016-08-24

Applicant: TRIANNI, INC.

Inventor： Matthias Wabl ,  Bao Duong

IPC: C07K16/46 ,  A01K67/027

CPC classification number: C07K16/468 ,  A01K67/0275 ,  A01K2217/07 ,  A01K2217/072 ,  A01K2227/105 ,  A01K2267/01

Abstract: The present invention provides cells, transgenic animals, including transgenic mammals and particularly rodents, comprising engineered immunoglobulin alleles. Mutations in the alleles are designed to compromise allelic exclusion and have potential to be exploited for the isolation of bispecific antibodies.

Abstract translation: 本发明提供细胞，转基因动物，包括转基因哺乳动物，特别是啮齿动物，包括工程化的免疫球蛋白等位基因。 等位基因中的突变被设计为损害等位基因排除并且有潜力被利用来分离双特异性抗体。