-
公开(公告)号：US20190127757A1
公开(公告)日：2019-05-02
申请号：US16165018
申请日：2018-10-19
Applicant: TRIANNI, INC.
Inventor： Matthias Wabl , Werner Mueller , Peter Burrows
Abstract: The invention relates to a long DH (LDH) cassette comprising a recombinant DH construct comprising at least two DH gene segments encoding at least 10 amino acids of the HCDR3 amino acid sequence, wherein at least one of the DH gene segments is a heterologous DH gene segment; an immunoglobulin heavy chain locus and a transgenic non-human animal comprising the same; and their use in producing an immunoglobulin library with long HCDR3 regions.
-
公开(公告)号：US12096753B2
公开(公告)日：2024-09-24
申请号：US17077545
申请日：2020-10-22
Applicant: Trianni, Inc.
Inventor： Matthias Wabl , Nigel Killeen
IPC: A01K67/0278 , A01K67/027 , C12N15/00 , C12N15/85 , C12P21/00
CPC classification number: A01K67/0278 , A01K67/027 , C12N15/00 , C12N15/8509 , C12P21/00 , A01K2217/07 , A01K2217/15 , A01K2227/105 , A01K2267/01 , C12N2800/30
Abstract: The present invention comprises non-human vertebrate cells and non-human mammals having a genome comprising an introduced partially human immunoglobulin region, said introduced region comprising human VH coding sequences and non-coding VH sequences based on the endogenous genome of the non-human mammal.
-
公开(公告)号：US12049516B2
公开(公告)日：2024-07-30
申请号：US16849847
申请日：2020-04-15
Applicant: TRIANNI, INC.
Inventor： Matthias Wabl
IPC: C07K16/46 , A01K67/0278 , C07K16/00 , A61K39/00
CPC classification number: C07K16/462 , A01K67/0278 , C07K16/00 , C12Y304/24046 , A01K2217/072 , A01K2217/15 , A01K2227/105 , A61K2039/552 , C07K2317/20 , C07K2317/24 , C07K2317/51 , C07K2317/515 , C07K2317/56
Abstract: The present invention relates to transgenic mammals that express canine-based immunoglobulins, including transgenic rodents that express canine-based immunoglobulins for the development of canine therapeutic antibodies.
-
公开(公告)号：US11946063B2
公开(公告)日：2024-04-02
申请号：US16165018
申请日：2018-10-19
Applicant: TRIANNI, INC.
Inventor： Matthias Wabl , Werner Mueller , Peter Burrows , Gloria Esposito , Bao Duong
IPC: C12N15/85 , A01K67/027 , A01K67/0278 , C07K16/18 , C12N5/074 , C12N15/10 , C12N15/62 , C40B40/10
CPC classification number: C12N15/8509 , A01K67/0278 , C07K16/18 , C12N5/0696 , C12N15/1072 , C12N15/62 , C40B40/10 , A01K2207/15 , A01K2217/054 , A01K2217/072 , A01K2227/105 , A01K2267/01 , A01K2267/03 , C12N2015/8518 , C12N2015/8527
Abstract: The invention relates to a long DH (LDH) cassette comprising a recombinant DH construct comprising at least two DH gene segments encoding at least 10 amino acids of the HCDR3 amino acid sequence, wherein at least one of the DH gene segments is a heterologous DH gene segment; an immunoglobulin heavy chain locus and a transgenic non-human animal comprising the same; and their use in producing an immunoglobulin library with long HCDR3 regions.
-
公开(公告)号：US10793829B2
公开(公告)日：2020-10-06
申请号：US15603347
申请日：2017-05-23
Applicant: Trianni, Inc
Inventor： Matthias Wabl
IPC: A01K67/027 , C12N5/0735 , C07K16/46 , C12N15/85
Abstract: The present invention relates to transgenic mammals that express bovine-based immunoglobulins, including transgenic rodents that express bovine-based immunoglobulins for the development of bovine therapeutic antibodies.
-
公开(公告)号：US20190053474A1
公开(公告)日：2019-02-21
申请号：US15781025
申请日：2016-11-30
Applicant: Trianni, Inc
Inventor： Matthias Wabl , Bao Duong , Nigel Killeen
IPC: A01K67/027 , C12N15/11 , C12N5/12 , C12N15/85 , C07K16/00
CPC classification number: A01K67/0278 , A01K2217/072 , A01K2227/105 , A01K2267/01 , A01K2267/03 , C07K16/00 , C07K2317/52 , C07K2317/60 , C12N5/12 , C12N15/11 , C12N15/85
Abstract: The invention provides compositions and methods for enhanced production of immunoglobulin diversity. Specifically, the invention provides compositions and methods for making accessible a B cell receptor repertoire that has not been culled by developmental tolerance mechanisms. The invention also provides transgenic animals, cells, and antibodies resulting from these compositions and methods.
-
公开(公告)号：US20170303517A1
公开(公告)日：2017-10-26
申请号：US15603347
申请日：2017-05-23
Applicant: Trianni, Inc
Inventor： Matthias Wabl
IPC: A01K67/027 , C12N15/85 , C07K16/18 , C12N5/0735
Abstract: The present invention relates to transgenic mammals that express bovine-based immunoglobulins, including transgenic rodents that express bovine-based immunoglobulins for the development of bovine therapeutic antibodies.
-
公开(公告)号：US20170058052A1
公开(公告)日：2017-03-02
申请号：US15246181
申请日：2016-08-24
Applicant: TRIANNI, INC.
Inventor： Matthias Wabl , Bao Duong
IPC: C07K16/46 , A01K67/027
CPC classification number: C07K16/468 , A01K67/0275 , A01K2217/07 , A01K2217/072 , A01K2227/105 , A01K2267/01
Abstract: The present invention provides cells, transgenic animals, including transgenic mammals and particularly rodents, comprising engineered immunoglobulin alleles. Mutations in the alleles are designed to compromise allelic exclusion and have potential to be exploited for the isolation of bispecific antibodies.
Abstract translation: 本发明提供细胞，转基因动物，包括转基因哺乳动物，特别是啮齿动物，包括工程化的免疫球蛋白等位基因。 等位基因中的突变被设计为损害等位基因排除并且有潜力被利用来分离双特异性抗体。
-
公开(公告)号：US11889821B2
公开(公告)日：2024-02-06
申请号：US17030057
申请日：2020-09-23
Applicant: TRIANNI, INC.
Inventor： Matthias Wabl , Bao Duong , Werner Mueller
IPC: A01K67/027 , C07K16/00 , C12N15/85 , C12N5/12 , C12N15/11
CPC classification number: A01K67/0278 , C07K16/00 , C12N5/12 , C12N15/11 , C12N15/85 , A01K2217/072 , A01K2227/105 , A01K2267/01 , A01K2267/03 , C07K2317/52 , C07K2317/60
Abstract: The invention provides compositions and methods for enhanced production of immunoglobulin diversity. Specifically, the invention provides compositions and methods for making accessible a B cell receptor repertoire that has not been culled by developmental tolerance mechanisms. The invention also provides transgenic animals, cells, and antibodies resulting from these compositions and methods.
-
公开(公告)号：US11414478B2
公开(公告)日：2022-08-16
申请号：US16631437
申请日：2018-07-20
Applicant: TRIANNI, INC.
Inventor： Werner Mueller , Matthias Wabl
IPC: C07K16/00 , A01K67/027
Abstract: The present invention provides cells, transgenic animals, including transgenic mammals and particularly rodents comprising engineered immunoglobulin (Ig) alleles. Such engineered alleles, wherein an Ig light chain CL exon [Cκ or Cλ (Cλ1, Cλ2 or Cλ3)] is incorporated into the Ig heavy chain locus, are capable of producing heavy chain-only antibodies as a single chain VH antibody (scVHAb) or heavy chain antibody (HCAb) comprising two extended scVHAbs. The scVHAb comprises an antigen-binding part consisting of a VH domain and the immunoglobulin constant domains CL, which is either Cκ or Cλ, and CH1, in the order from N-terminus to C-terminus: VH-L1-CL-L2-CH1, wherein L1 and L2 are each, independently, peptidic linkers; and wherein CL is paired with CH1 through beta-sheet contact thereby obtaining a CL/CH1 dimer.
