公开(公告)号：US20040115718A1

公开(公告)日：2004-06-17

申请号：US10687588

申请日：2003-10-20

Applicant: TOSOH CORPORATION

Inventor： Takahiko Ishiguro ,  Juichi Saitoh ,  Tetsuya Ishizuka

IPC: C12Q001/68 ,  C12P019/34

CPC classification number: C12Q1/686 ,  C12Q1/6865 ,  C12Q2563/173 ,  C12Q2527/125 ,  C12Q2521/107 ,  C12Q2521/119 ,  C12Q2563/107

Abstract: A simple and accurate method for assay of a single-stranded RNA containing a specific nucleic acids sequence in a sample at almost constant temperature by using at least the following reagents (A) to (I), which comprises a step of adding the reagents (A) to (I) one by one (in any order), in combinations of at least two or all at once and a step of measuring a fluorescent signal in the presence of the reagent (I) at least once after addition of at least the reagents (A) to (H); (A) a first single-stranded oligonucleic acid complementary to a sequence neighboring the 5null end of the specific nucleic acids sequence in the single-stranded RNA, (B) a second single-stranded oligo DNA complementary to a 3null-end sequence within the specific nucleic acids sequence, (C) an RNA-dependent DNA polymerase, (D) deoxyribonucleoside triphosphates, (E) a third single-stranded oligo DNA having (1) a promoter sequence for a DNA-dependent RNA polymerase, (2) an enhancer sequence for the promoter and (3) a 5null-end sequence within the specific nucleic acids sequence, in this order from the 5null end, (F) a DNA-dependent DNA polymerase, (G) a DNA-dependent RNA polymerase, (H) ribonucleoside triphosphates, and (I) a fourth single-stranded oligo DNA complementary to the specific nucleic acids sequence which is labeled so that it gives off a measurable fluorescent signal on hybridization with a nucleic acid containing the specific nucleic acids sequence.

Abstract translation: 通过使用至少以下试剂（A）至（I），在几乎恒定温度下测定样品中含有特定核酸序列的单链RNA的简单且准确的方法，其包括将试剂（ A）至（I）（以任何顺序），至少两个或全部同时组合，以及在至少加入至少一个试剂（I）之后至少测量一次荧光信号的步骤 试剂（A）〜（H）; （A）与单链RNA中特异性核酸序列的5'末端相邻的序列互补的第一单链寡核酸，（B）与3'-末端序列互补的第二单链寡核苷酸 在特定的核酸序列中，（C）RNA依赖性DNA聚合酶，（D）脱氧核糖核苷三磷酸，（E）具有（1）DNA依赖性RNA聚合酶的启动子序列的第三单链寡核苷酸（2） ）启动子的增强子序列和（3）特异性核酸序列内的5'端序列，从5'端起依次为（F）DNA依赖性DNA聚合酶，（G）DNA依赖性 RNA聚合酶，（H）核糖核苷三磷酸和（I）与特异性核酸序列互补的第四单链寡核苷酸，其被标记以便在与含有特定核酸的核酸杂交时发出可测量的荧光信号 序列。

公开(公告)号：US20030026705A1

公开(公告)日：2003-02-06

申请号：US10174993

申请日：2002-06-20

Applicant: TOSOH CORPORATION

Inventor： Takahiko Ishiguro ,  Juichi Saitoh ,  Yoshifumi Kurihara

IPC: F04F001/00

CPC classification number: F04B17/00 ,  B01L3/50273 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2400/043 ,  F04B19/006

Abstract: The present invention provides a simple liquid transportation method without requiring high electric voltage or special external equipment, and a method for transporting liquid based on this transportation method. The liquid transportation method comprises a step of introducing a magnetic fluid and a liquid to be transported into a conduit or a chamber connected thereto so that the magnetic fluid and the liquid to be transported are in contact with each other directly or indirectly via a medium, a step of moving the magnetic fluid by applying a magnetic field, and a step of moving above-mentioned liquid to be transported by letting it follow the movement of the magnetic fluid. The present invention also provides a simple transportation method of a specific amount of liquid without requiring high voltage or incorporation of specific actuator components such as valves. Using a conduit wherein at least three microchannels intersect one another and at least two of them have openings which can be opened or closed, opening and closing of the openings and liquid transportation are carried out in a controlled order, so that only a specific amount from a liquid introduced in an unspecific amount, is transported.

Abstract translation: 本发明提供一种不需要高电压或特殊外部设备的简单的液体输送方法，以及基于该输送方法的液体输送方法。 液体输送方法包括将待输送的磁性流体和液体引入到与其连接的导管或室中的步骤，使得磁性流体和待输送的液体通过介质直接或间接接触， 通过施加磁场来移动磁性流体的步骤，以及通过使上述待运输的液体跟随磁性流体的运动而移动上述待运送的液体的步骤。 本发明还提供了特定量的液体的简单的运输方法，而不需要高电压或并入诸如阀门的特定致动器部件。 使用其中至少三个微通道彼此相交并且其中至少两个具有可以打开或关闭的开口的管道，开口的开闭和液体输送以受控的顺序进行，使得只有特定量的 以非特定的量引入的液体被输送。

公开(公告)号：US20020155619A1

公开(公告)日：2002-10-24

申请号：US10126629

申请日：2002-04-22

Applicant: TOSOH CORPORATION

Inventor： Yoshifumi Kurihara ,  Toshinori Hayashi ,  Juichi Saitoh ,  Takahiko Ishiguro

IPC: G01N021/76

CPC classification number: G01N21/6452 ,  G01N2201/0642 ,  Y10T436/25 ,  Y10T436/2575

Abstract: A method for detecting fluorescence from a liquid sample housed in a transparent or translucent sample container includes providing a sample container in a sample holder, which is opaque except for a sample container introduction opening and incoming and outgoing openings for excitation light; layering a liquid sample and a shielding liquid unmixable therewith to prevent external light from entering through the sample container introduction opening; introducing excitation light from such a direction that the excitation light can irradiate the liquid sample before irradiating the shielding liquid; and detecting fluorescence, which emits in a direction of avoiding absorption by the shielding liquid.

Abstract translation: 用于检测容纳在透明或半透明样品容器中的液体样品的荧光的方法包括：将样品容器放置在样品保持器中，样品容器除了样品容器引入开口和用于激发光的入射和出射开口外是不透明的; 层叠液体样品和与其不可混合的屏蔽液体，以防止外部光线通过样品容器引入口; 在照射所述屏蔽液体之前，从所述激发光可以照射所述液体样品的方向引入激发光; 并检测荧光，其沿避开被屏蔽液体吸收的方向发射。

公开(公告)号：US20040014119A1

公开(公告)日：2004-01-22

申请号：US10465607

申请日：2003-06-20

Applicant: TOSOH CORPORATION

Inventor： Shun-Ichi Itoh ,  Toshinori Hayashi ,  Juichi Saitoh ,  Takahiko Ishiguro

IPC: C12Q001/68

CPC classification number: C12Q1/6816 ,  C12Q1/686 ,  C12Q2565/107 ,  C12Q2527/107

Abstract: A method of detecting at least two nucleic acids in a sample, which comprises allowing the respective nucleic acids to form duplexes having different Tm values with nucleic acid probes complementary to the respective nucleic acids which are labeled with the same fluorescent substance and detecting the respective nucleic acids in one vessel simultaneously on the basis of the different Tm values of the duplexes.

Abstract translation: 一种检测样品中至少两种核酸的方法，其包括使各个核酸形成具有不同Tm值的双链体，其中核酸探针与用相同荧光物质标记的相应核酸互补并检测相应的核酸 基于双链体的不同Tm值同时在一个容器中的酸。