公开(公告)号：US20040014119A1

公开(公告)日：2004-01-22

申请号：US10465607

申请日：2003-06-20

Applicant: TOSOH CORPORATION

Inventor： Shun-Ichi Itoh ,  Toshinori Hayashi ,  Juichi Saitoh ,  Takahiko Ishiguro

IPC: C12Q001/68

CPC classification number: C12Q1/6816 ,  C12Q1/686 ,  C12Q2565/107 ,  C12Q2527/107

Abstract: A method of detecting at least two nucleic acids in a sample, which comprises allowing the respective nucleic acids to form duplexes having different Tm values with nucleic acid probes complementary to the respective nucleic acids which are labeled with the same fluorescent substance and detecting the respective nucleic acids in one vessel simultaneously on the basis of the different Tm values of the duplexes.

Abstract translation: 一种检测样品中至少两种核酸的方法，其包括使各个核酸形成具有不同Tm值的双链体，其中核酸探针与用相同荧光物质标记的相应核酸互补并检测相应的核酸 基于双链体的不同Tm值同时在一个容器中的酸。