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62 条记录 (耗时 0.02 秒)

    Method for producing neural stem cells using synthetic peptide
    1.
    发明授权
    Method for producing neural stem cells using synthetic peptide 有权

    公开(公告)号：US10421944B2

    公开(公告)日：2019-09-24

    申请号：US15279890

    申请日：2016-09-29

    申请人： TOAGOSEI CO. LTD. ,  KEIO UNIVERSITY

    发明人： Nahoko Baileykobayashi ,  Kenichi Tanaka ,  Tetsuhiko Yoshida ,  Jun Kudo

    IPC分类号： C12N5/0797

    摘要： The composition for producing neural stem cells provided by the present invention contains an artificially synthesized synthetic peptide having a neural stem cell-inducing peptide sequence that induces neural stem cells from fibroblasts, and one or two or more pharmaceutically acceptable carriers. The neural stem cell-inducing peptide sequence is any of (i) an amino acid sequence constituting a signal peptide of any protein belonging to the amyloid precursor protein (APP) family, (ii) a partial amino acid sequence of the amino acid sequence of (i), and (iii) a modified amino acid sequence of the amino acid sequence of (i) or (ii).

    PEPTIDE FRAGMENT AND USE THEREOF
    6.
    发明公开
    PEPTIDE FRAGMENT AND USE THEREOF 审中-公开

    公开(公告)号：US20240262863A1

    公开(公告)日：2024-08-08

    申请号：US18557620

    申请日：2022-03-25

    申请人： TOAGOSEI CO., LTD.

    发明人： Nahoko Baileykobayashi ,  Tetsuhiko Yoshida

    IPC分类号： C07K7/08 ,  C12N15/87

    CPC分类号： C07K7/08 ,  C12N15/87

    摘要： The present disclosure provides an artificially synthesized peptide fragment capable of efficiently introducing a target foreign substance from outside a eukaryotic cell into at least the cytoplasm of the cell. A peptide fragment disclosed herein is for introducing a target foreign substance from outside a eukaryotic cell into at least the cytoplasm of the cell, the peptide fragment being configured such that two or more of amino acid sequences VVKSLVK (SEQ ID NO: 1) are arranged in series, and another amino acid residue is not included between the amino acid sequences or a peptide linker composed of 1 to 5 amino acid residues is interposed between the amino acid sequences.

    Carrier peptide fragment and use thereof
    7.
    发明授权
    Carrier peptide fragment and use thereof 有权

    公开(公告)号：US11814448B2

    公开(公告)日：2023-11-14

    申请号：US17525097

    申请日：2021-11-12

    申请人： TOAGOSEI CO., LTD.

    发明人： Nahoko Baileykobayashi ,  Tetsuhiko Yoshida

    IPC分类号： C07K7/08 ,  C07K14/00 ,  C12N5/09

    CPC分类号： C07K7/08 ,  C07K14/00 ,  C12N5/0693 ,  C07K2319/00

    摘要： The present disclosure provides an artificially synthesized peptide fragment that can efficiently introduce a foreign substance of interest from outside a eukaryotic cell into at least the cytoplasm of the cell. The present disclosure also provides a method for using the peptide fragment. The herein disclosed peptide fragment is a peptide fragment for introducing a foreign substance of interest from outside a eukaryotic cell into at least the cytoplasm of the cell, and is provided with a repeat sequence in which the VVRSLVK amino acid sequence (SEQ ID NO: 1) is repeated at least two or more times. By using a construct that has the peptide fragment and the foreign substance of interest bonded to the N-terminal side and/or C-terminal side of the peptide fragment, the foreign substance of interest can be efficiently introduced from outside a eukaryotic cell into at least the cytoplasm of the cell.

    Carrier Peptide Fragment for Nucleolar Localization and Use Thereof
    9.
    发明公开
    Carrier Peptide Fragment for Nucleolar Localization and Use Thereof 审中-公开

    公开(公告)号：US20230160876A1

    公开(公告)日：2023-05-25

    申请号：US17871833

    申请日：2022-07-22

    申请人： TOAGOSEI CO., LTD.

    发明人： Nahoko Baileykobayashi ,  Tetsuhiko Yoshida

    IPC分类号： G01N33/50 ,  C07K7/08

    CPC分类号： G01N33/5008 ,  C07K7/08

    摘要： According to the present disclosure, a technology for efficiently introducing a desired foreign substance from the outside of eukaryotic cells into at least the cytoplasm (and the nucleolus) of the cells is provided. A method disclosed here includes a step of preparing a construct for introducing a foreign substance including a carrier peptide fragment composed of any of amino acid sequences: KKRTLRKKKRKKR (SEQ ID NO: 1), KKRTLRKRRRKKR (SEQ ID NO: 2), KKRTLRKRKRKKR (SEQ ID NO: 3) and KKRTLRKKRRKKR (SEQ ID NO: 4), and a foreign substance that is bound to an N-terminal side and/or C-terminal side of the carrier peptide fragment, a step of supplying the construct for introducing a foreign substance to a sample containing desired eukaryotic cells, and a step of incubating the sample to which the construct for introducing a foreign substance is supplied and introducing the construct into eukaryotic cells in the sample.

    siRNA based on RNA sequence of SARS-CoV-2 and use thereof
    10.
    发明申请
    siRNA based on RNA sequence of SARS-CoV-2 and use thereof 有权

    公开(公告)号：US20230047473A1

    公开(公告)日：2023-02-16

    申请号：US17811961

    申请日：2022-07-12

    申请人： TOAGOSEI CO., LTD. ,  KYOTO UNIVERSITY

    发明人： Tetsuhiko Yoshida ,  Nahoko Baileykobayashi ,  Kazuo Takayama ,  Rina Hashimoto ,  Yoshinori Yoshida

    IPC分类号： C12N15/113

    摘要： The present disclosure provides siRNA that suppresses proliferation of new coronaviruses (SARS-CoV-2). The siRNA disclosed herein includes: a sense strand; and an antisense strand. The sense strand includes a target sequence comprising 19 to 23 bases in which a base at a 5′ terminal is guanine (G) or cytosine (C), and an overhang comprising 2 to 4 bases added to a 3′ terminal side of the target sequence. The antisense strand includes a sequence complementary to the target sequence, and an overhang comprising 2 to 4 bases added to a 3′ terminal side of the complementary sequence. Here, at least a part of the target sequence contains at least a part of a base sequence encoding a signal peptide region of a spike protein (S protein) of SARS-CoV-2.