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公开(公告)号：US11859162B2
公开(公告)日：2024-01-02
申请号：US16557820
申请日：2019-08-30
Applicant: The Charles Stark Draper Laboratory, Inc.
Inventor： Vishal Tandon , Charles A. Lissandrello , Jenna L. Balestrini , Jonathan R. Coppeta , Patricia A. Swierk
CPC classification number: C12M23/16 , C12M35/02 , B01L3/50273 , B01L3/502715 , B01L2300/047 , B01L2300/087 , B01L2300/0864 , B01L2300/0887 , B01L2400/0496 , C12N15/87
Abstract: Transfer of genetic and other materials to cells is conducted in a hands-free, automated, high throughput, continuous process. A system using a microfluidic hydrodynamic sheath flow configuration includes arrangements for pushing cells from side streams containing a cell culture medium to a central stream containing an electroporation buffer. Electroporation can be conducted in an assembly in which two or more microfluidic channels are provided in a parallel configuration and in which various layers can be stacked together to form a laminate type structure.
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公开(公告)号：US11261465B2
公开(公告)日：2022-03-01
申请号：US16026709
申请日：2018-07-03
Applicant: The Charles Stark Draper Laboratory, Inc.
Inventor： Vishal Tandon , Daniel K. Freeman , Jonathan R. Coppeta , Jeffrey T. Borenstein , Jenna L. Balestrini
Abstract: A method and system of delivering a charged cargo, such as a biomolecule, to a target structure, such as cells, exosomes, other vesicles or micelles, using an electroactive porous membrane. This method comprises contacting an electroactive porous membrane with a fluid flow toward the membrane. The fluid contains charged biomolecules and the membrane and biomolecules are oppositely charged so that the biomolecules in the fluid are trapped on the membrane as the fluid flows through the pores of the membrane. Acceptor cells of interest are pinned to the membrane by the flow of the fluid, thereby aggregating the cells onto the membrane in close proximity to the trapped biomolecules. Finally, the acceptor cells are permeabilized.
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公开(公告)号：US20210155889A1
公开(公告)日：2021-05-27
申请号：US17100379
申请日：2020-11-20
Applicant: The Charles Stark Draper Laboratory, Inc.
Inventor： Vishal Tandon , Jeffrey Borenstein , Jason Fiering , Jenna Balestrini , Heena Mutha , Jonathan Robert Coppeta , Mark Mescher
Abstract: A system for cell bioprocessing and cell therapy manufacturing can include a series of microfluidic modules to enable continuous-flow end-to-end cell bioprocessing. Each module can implement a different technology, and the modules can be coupled to one another to perform various unit operations in the cell bioprocessing or cell-therapy manufacturing chain to enable direct processing of a blood or blood product sample. The system can automatically and continuously process the sample into genetically-modified lymphocytes or T cells for cellular therapy. The technologies implemented by each module in the system can include any combination of microfluidic acoustophoresis, microfluidic acoustophoretic media exchange or cell washing, and continuous-flow microfluidic electrotransfection. Modules implementing these microfluidic technologies can be interconnected with plastic tubing or with a custom manifold.
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公开(公告)号：US10214750B2
公开(公告)日：2019-02-26
申请号：US15614421
申请日：2017-06-05
Applicant: The Charles Stark Draper Laboratory, Inc.
Inventor： Jeffrey T. Borenstein , Joseph L. Charest , Christopher M. DiBiasio , Dorit Berlin , Jenna Balestrini , Jose A. Santos , Vishal Tandon
Abstract: Systems and methods are disclosed herein for use in transducing, activating, and otherwise treating cells. Cells are introduced into an inner layer of a multi-layered stack that defines at least one flow chamber and a plurality of cell entrainment regions. Vertical flow through the stack entrains the cells in the cell entrainment regions along with genetic information introduction agents or other additives, before the cells are washed using a reverse vertical flow and are collected from the device.
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公开(公告)号：US12139697B2
公开(公告)日：2024-11-12
申请号：US17946948
申请日：2022-09-16
Applicant: THE CHARLES STARK DRAPER LABORATORY, INC.
Inventor： Kenneth T. Kotz , Bryan D. Teece , James Gillett Truslow , Nathan Francis Moore , Jeffrey T. Borenstein , Vishal Tandon
IPC: C12M1/02 , C12M1/00 , C12M1/12 , C12M1/34 , C12M1/40 , C12M3/06 , C12N15/86 , C12N15/87 , A61K48/00
Abstract: A device for treatment of cells with particles is disclosed. The device includes a semi-permeable membrane positioned between two plates, the first plate defining a first flow chamber and comprising a port, a flow channel, a transverse port, and a transverse flow channel, the first flow chamber constructed and arranged to deliver fluid in a transverse direction along the first side of the semi-permeable membrane, the second plate defining a second flow chamber and comprising a port. A method for transducing cells is disclosed. The method includes introducing a fluid with cells and viral particles into a flow chamber adjacent a semi-permeable membrane such that the cells and the viral particles are substantially evenly distributed on the semi-permeable membrane. The method also includes introducing a recovery fluid to suspend the cells and the viral particles, and separating the cells from the viral particles. A method of activating cells is disclosed.
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Patent Agency Ranking
6.发明公开公开(公告)号：US20240131513A1
公开(公告)日：2024-04-25
申请号：US18515386
申请日：2023-11-21
Applicant: The Charles Stark Draper Laboratory, Inc.
Inventor： Jonathan R. Coppeta , Timothy J. Biliouris , Daniel F. King , Vishal Tandon
CPC classification number: B01L3/502715 , B01L3/50273 , C12N13/00 , C12N15/87 , B01L2200/0647 , B01L2300/06 , B01L2300/0645 , B01L2300/0809 , B01L2400/0421 , B01L2400/0424
Abstract: A device, system and process involve conducting electroporation of microvesicles or exosomes or other target structures in a microfluidic arrangement at pressures that exceed atmospheric pressure. Single as well as multiple flow configurations can be employed. In some cases, the system and its operation are computer-controlled for partial or complete automation.
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7.发明申请公开(公告)号：US20200070163A1
公开(公告)日：2020-03-05
申请号：US16560707
申请日：2019-09-04
Applicant: The Charles Stark Draper Laboratory, Inc.
Inventor： Jonathan R. Coppeta , Timothy J. Biliouris , Daniel F. King , Vishal Tandon
Abstract: A device, system and process involve conducting electroporation of microvesicles or exosomes or other target structures in a microfluidic arrangement at pressures that exceed atmospheric pressure. Single as well as multiple flow configurations can be employed. In some cases, the system and its operation are computer-controlled for partial or complete automation.
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公开(公告)号：US20190292565A1
公开(公告)日：2019-09-26
申请号：US16359626
申请日：2019-03-20
Applicant: The Charles Stark Draper Laboratory, Inc.
Inventor： Vishal Tandon , Charles A. Lissandrello , Jenna Leigh Balestrini , Ryan A. Dubay
Abstract: A system for sequential exposure of particles to different fluid streams includes an acoustic actuator device for acoustically driving one or more substrates and a microchannel device of the one or more substrates that receive particles in a first flowing fluid, moves the particles to a second flowing fluid, then moves the particles out of the second flowing fluid using acoustic radiation generated by the acoustic actuator device. The system can control residence times in the streams. According to one use, the first flowing fluid is a cell buffer and the second flowing media is an electroporation buffer. An electroporation system is placed in or downstream of the acoustic actuator device. However, in other uses, the second flowing media might be a wash buffer.
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9.发明授权公开(公告)号：US11878300B2
公开(公告)日：2024-01-23
申请号：US16560707
申请日：2019-09-04
Applicant: The Charles Stark Draper Laboratory, Inc.
Inventor： Jonathan R. Coppeta , Timothy J. Biliouris , Daniel F. King , Vishal Tandon
CPC classification number: B01L3/502715 , B01L3/50273 , C12N13/00 , C12N15/87 , B01L2200/0647 , B01L2300/06 , B01L2300/0645 , B01L2300/0809 , B01L2400/0421 , B01L2400/0424
Abstract: A device, system and process involve conducting electroporation of microvesicles or exosomes or other target structures in a microfluidic arrangement at pressures that exceed atmospheric pressure. Single as well as multiple flow configurations can be employed. In some cases, the system and its operation are computer-controlled for partial or complete automation.
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公开(公告)号：US20230080444A1
公开(公告)日：2023-03-16
申请号：US17946862
申请日：2022-09-16
Applicant: THE CHARLES STARK DRAPER LABORATORY, INC.
Inventor： Kenneth T. Kotz , Bryan D. Teece , James Gillett Truslow , Nathan Francis Moore , Jeffrey T. Borenstein , Vishal Tandon
Abstract: A device for treatment of cells with particles is disclosed. The device includes a semi-permeable membrane positioned between two plates, the first plate defining a first flow chamber and comprising a port, a flow channel, a transverse port, and a transverse flow channel, the first flow chamber constructed and arranged to deliver fluid in a transverse direction along the first side of the semi-permeable membrane, the second plate defining a second flow chamber and comprising a port. A method for transducing cells is disclosed. The method includes introducing a fluid with cells and viral particles into a flow chamber adjacent a semi-permeable membrane such that the cells and the viral particles are substantially evenly distributed on the semi-permeable membrane. The method also includes introducing a recovery fluid to suspend the cells and the viral particles, and separating the cells from the viral particles. A method of activating cells is disclosed.
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