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公开(公告)号：US12139697B2
公开(公告)日：2024-11-12
申请号：US17946948
申请日：2022-09-16
Applicant: THE CHARLES STARK DRAPER LABORATORY, INC.
Inventor： Kenneth T. Kotz , Bryan D. Teece , James Gillett Truslow , Nathan Francis Moore , Jeffrey T. Borenstein , Vishal Tandon
IPC: C12M1/02 , C12M1/00 , C12M1/12 , C12M1/34 , C12M1/40 , C12M3/06 , C12N15/86 , C12N15/87 , A61K48/00
Abstract: A device for treatment of cells with particles is disclosed. The device includes a semi-permeable membrane positioned between two plates, the first plate defining a first flow chamber and comprising a port, a flow channel, a transverse port, and a transverse flow channel, the first flow chamber constructed and arranged to deliver fluid in a transverse direction along the first side of the semi-permeable membrane, the second plate defining a second flow chamber and comprising a port. A method for transducing cells is disclosed. The method includes introducing a fluid with cells and viral particles into a flow chamber adjacent a semi-permeable membrane such that the cells and the viral particles are substantially evenly distributed on the semi-permeable membrane. The method also includes introducing a recovery fluid to suspend the cells and the viral particles, and separating the cells from the viral particles. A method of activating cells is disclosed.
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公开(公告)号：US20240110908A1
公开(公告)日：2024-04-04
申请号：US18528061
申请日：2023-12-04
Applicant: The Charles Stark Draper Laboratory, Inc.
Inventor： Jason O. Fiering , Kenneth T. Kotz
CPC classification number: G01N33/491 , A61M1/3678 , A61M1/3693 , B01D21/283 , B01L3/502753 , B03B1/04 , G01N15/02 , G01N15/06 , B01D2221/10 , B01L2400/0436 , G01N2015/0065
Abstract: A method for separating particles in a biofluid includes pretreating the biofluid by introducing an additive, flowing the pretreated biofluid through a microfluidic separation channel, and applying acoustic energy to the microfluidic separation channel. A system for microfluidic separation, capable of separating target particles from non-target particles in a biofluid includes at least one microfluidic separation channel, a source of biofluid, a source of additive, and at least one acoustic transducer coupled to the microfluidic separation channel. A kit for microfluidic particle separation includes a microfluidic separation channel connected to an acoustic transducer, a source of an additive, and instructions for use.
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公开(公告)号：US20230063282A1
公开(公告)日：2023-03-02
申请号：US17946948
申请日：2022-09-16
Applicant: THE CHARLES STARK DRAPER LABORATORY, INC.
Inventor： Kenneth T. Kotz , Bryan D. Teece , James Gillett Truslow , Nathan Francis Moore , Jeffrey T. Borenstein , Vishal Tandon
Abstract: A device for treatment of cells with particles is disclosed. The device includes a semi-permeable membrane positioned between two plates, the first plate defining a first flow chamber and comprising a port, a flow channel, a transverse port, and a transverse flow channel, the first flow chamber constructed and arranged to deliver fluid in a transverse direction along the first side of the semi-permeable membrane, the second plate defining a second flow chamber and comprising a port. A method for transducing cells is disclosed. The method includes introducing a fluid with cells and viral particles into a flow chamber adjacent a semi-permeable membrane such that the cells and the viral particles are substantially evenly distributed on the semi-permeable membrane. The method also includes introducing a recovery fluid to suspend the cells and the viral particles, and separating the cells from the viral particles. A method of activating cells is disclosed.
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公开(公告)号：US20210293781A1
公开(公告)日：2021-09-23
申请号：US17171651
申请日：2021-02-09
Applicant: THE CHARLES STARK DRAPER LABORATORY, INC.
Inventor： Jason O. Fiering , Kenneth T. Kotz
Abstract: A method for separating particles in a biofluid includes pretreating the biofluid by introducing an additive, flowing the pretreated biofluid through a microfluidic separation channel, and applying acoustic energy to the microfluidic separation channel. A system for microfluidic separation, capable of separating target particles from non-target particles in a biofluid includes at least one microfluidic separation channel, a source of biofluid, a source of additive, and at least one acoustic transducer coupled to the microfluidic separation channel. A kit for microfluidic particle separation includes a microfluidic separation channel connected to an acoustic transducer, a source of an additive, and instructions for use.
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公开(公告)号：US11291756B2
公开(公告)日：2022-04-05
申请号：US16452191
申请日：2019-06-25
Applicant: THE CHARLES STARK DRAPER LABORATORY, INC.
Inventor： Jason O. Fiering , Kenneth T. Kotz , Nathan Francis Moore
Abstract: A method for separating cells in a biofluid includes pretreating the biofluid by introducing an additive comprising a cell activator, flowing the pretreated biofluid through a microfluidic separation channel, and applying acoustic energy to the microfluidic separation channel to accumulate target cells in a primary stream and non-target cells in a secondary stream. A system for microfluidic cell separation capable of separating target cells from non-target cells in a biofluid includes at least one microfluidic separation channel, a source of biofluid, a source of additive comprising a cell activator, and at least one acoustic transducer coupled to the microfluidic separation channel.
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Patent Agency Ranking
公开(公告)号：US20190290829A1
公开(公告)日：2019-09-26
申请号：US16302429
申请日：2017-04-28
Applicant: THE CHARLES STARK DRAPER LABORATORY, INC.
Inventor： Jason O. Fiering , Kenneth T. Kotz
Abstract: A method for separating cells in a biofluid includes pretreating the biofluid by introducing an additive, flowing the pretreated biofluid through a microfluidic separation channel, and applying acoustic energy to the microfluidic separation channel. A system for microfluidic cell separation, capable of separating target cells from non-target cells in a biofluid includes at least one microfluidic separation channel, a source of biofluid, a source of additive, and at least one acoustic transducer coupled to the microfluidic separation channel. A kit for microfluidic cell separation includes a microfluidic separation channel connected to an acoustic transducer, a source of an additive, and instructions for use.
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公开(公告)号：US20190085280A1
公开(公告)日：2019-03-21
申请号：US16137478
申请日：2018-09-20
Applicant: THE CHARLES STARK DRAPER LABORATORY, INC.
Inventor： Kenneth T. Kotz , Bryan D. Teece , James Gillett Truslow , Nathan Francis Moore
Abstract: A device for treatment of cells with particles is disclosed. The device includes a semi-permeable membrane positioned between two plates, the first plate defining a first flow chamber and comprising a port, a flow channel, a transverse port, and a transverse flow channel, the first flow chamber constructed and arranged to deliver fluid in a transverse direction along the first side of the semi-permeable membrane, the second plate defining a second flow chamber and comprising a port. A method for transducing cells is disclosed. The method includes introducing a fluid with cells and viral particles into a flow chamber adjacent a semi-permeable membrane such that the cells and the viral particles are substantially evenly distributed on the semi-permeable membrane. The method also includes introducing a recovery fluid to suspend the cells and the viral particles, and separating the cells from the viral particles. A method of activating cells is disclosed.
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公开(公告)号：US11835513B2
公开(公告)日：2023-12-05
申请号：US17171651
申请日：2021-02-09
Applicant: THE CHARLES STARK DRAPER LABORATORY, INC.
Inventor： Jason O. Fiering , Kenneth T. Kotz
IPC: G01N33/49 , A61M1/36 , B01D21/28 , G01N15/02 , B01L3/00 , G01N15/06 , B03B1/04 , G01N15/00 , G01N15/10
CPC classification number: G01N33/491 , A61M1/3678 , A61M1/3693 , B01D21/283 , B01L3/502753 , B03B1/04 , G01N15/02 , G01N15/06 , B01D2221/10 , B01L3/502761 , B01L2200/0652 , B01L2400/0436 , G01N2015/0065 , G01N2015/0288 , G01N2015/0681 , G01N2015/0687 , G01N2015/1006 , G01N2015/1081
Abstract: A method for separating particles in a biofluid includes pretreating the biofluid by introducing an additive, flowing the pretreated biofluid through a microfluidic separation channel, and applying acoustic energy to the microfluidic separation channel. A system for microfluidic separation, capable of separating target particles from non-target particles in a biofluid includes at least one microfluidic separation channel, a source of biofluid, a source of additive, and at least one acoustic transducer coupled to the microfluidic separation channel. A kit for microfluidic particle separation includes a microfluidic separation channel connected to an acoustic transducer, a source of an additive, and instructions for use.
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公开(公告)号：US20200057045A1
公开(公告)日：2020-02-20
申请号：US16661845
申请日：2019-10-23
Applicant: THE CHARLES STARK DRAPER LABORATORY, INC.
Inventor： Jason O. Fiering , Kenneth T. Kotz
Abstract: A method for separating particles in a biofluid includes pretreating the biofluid by introducing an additive, flowing the pretreated biofluid through a microfluidic separation channel, and applying acoustic energy to the microfluidic separation channel. A system for microfluidic separation, capable of separating target particles from non-target particles in a biofluid includes at least one microfluidic separation channel, a source of biofluid, a source of additive, and at least one acoustic transducer coupled to the microfluidic separation channel. A kit for microfluidic particle separation includes a microfluidic separation channel connected to an acoustic transducer, a source of an additive, and instructions for use.
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公开(公告)号：US20190354871A1
公开(公告)日：2019-11-21
申请号：US16415666
申请日：2019-05-17
Applicant: The Charles Stark Draper Laboratory, Inc.
Inventor： Kirsty A. McFarland , Andrew P. Magyar , Ian Ward Frank , Kenneth T. Kotz , Stacey Markovic , Haiyao Huang , Steven J. Byrnes , Gregg E. Favalora , Melissa M. Sprachman , Ryan A. Dubay , Emma Vargo , Peter Cavanagh , Erin Rosenberger
Abstract: A parallelized chain-synthesizing technique includes capillary tubes, where each tube provides multiple locations or addresses where a specific arbitrary sequence for polymeric chains can be synthesized. An optical addressing system selectively delivers light to the locations to mediate or control reactions in the tubes.
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