公开(公告)号：US20210123066A1

公开(公告)日：2021-04-29

申请号：US16963133

申请日：2019-01-17

Applicant: TEMASEK LIFE SCIENCES LABORATORY LIMITED

Inventor： In-Cheol JANG ,  Jun-shi ZHENG ,  Mi Jung KIM

IPC: C12N15/82 ,  A01H4/00

Abstract: The present invention relates to a method for Agrobacterium-mediated transformation and regeneration of Stevia plants. In particular, the method involves co-culturing leaf explants with Agrobacterium in a medium comprising acetosyringone and 2,4-dichlorophenoxyacetic acid in the dark, callus induction and shoot regeneration in a medium comprising 6-benzylaminopurine, 3-indoleacetic acid, a selective agent and an Agrobacterium eradicant in the dark, and root regeneration in a medium comprising 3-indoleacetic acid in a light/dark cycle. The present invention also relates to the overexpression of SrDXS1 and SrKAH in transgenic plants, resulting in the enhancement of steviol glycosides in the transgenic plants. The present invention further relates to the overexpression SrUGT76G1 in transgenic plants, resulting in higher Rebaudioside A (Reb A) to stevioside ratios in the transgenic plants.

公开(公告)号：US20170253885A1

公开(公告)日：2017-09-07

申请号：US15519595

申请日：2015-10-19

Applicant: TEMASEK LIFE SCIENCES LABORATORY LIMITED

Inventor： In-cheol JANG ,  Mi Jung KIM

IPC: C12N15/82 ,  C12P5/00 ,  C12N9/88

CPC classification number: C12N15/8243 ,  C12N9/18 ,  C12N9/88 ,  C12P5/007 ,  C12P7/04 ,  C12Y402/03

Abstract: The present invention relates to the field of plant molecular biology. More particularly, the present invention relates to the isolation of nucleic acids encoding terpene synthases (TPSs), including a novel, multifunctional TPS identified herein as CoTPS2.

公开(公告)号：US20220098601A2

公开(公告)日：2022-03-31

申请号：US16963133

申请日：2019-01-17

Applicant: TEMASEK LIFE SCIENCES LABORATORY LIMITED

Inventor： In-Cheol JANG ,  Jun-shi ZHENG ,  Mi Jung KIM

IPC: C12N15/82 ,  A01H4/00

Abstract: The present invention relates to a method for Agrobacterium-mediated transformation and regeneration of Stevia plants. In particular, the method involves co-culturing leaf explants with Agrobacterium in a medium comprising acetosyringone and 2,4-dichlorophenoxyacetic acid in the dark, callus induction and shoot regeneration in a medium comprising 6-benzylaminopurine, 3-indoleacetic acid, a selective agent and an Agrobacterium eradicant in the dark, and root regeneration in a medium comprising 3-in-doleacetic acid in a light/dark cycle. The present invention also relates to the overexpression of SrDXS I and SrKAH in transgenic plants, resulting in the enhancement of steviol glycosides in the transgenic plants. The present invention further relates to the overexpression SrUGT76G I in transgenic plants, resulting in higher Rebaudioside A (Reb A) to stevioside ratios in the transgenic plants.