公开(公告)号：US20230243750A1

公开(公告)日：2023-08-03

申请号：US17999331

申请日：2021-05-20

Applicant: TEMASEK LIFE SCIENCES LABORATORY LIMITED ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY

Inventor： In-Cheol JANG ,  Benny Jian Rong SNG ,  Gajendra Pratap SINGH ,  Rajeev J. RAM

IPC: G01N21/65 ,  G01N21/84 ,  G01N33/00

CPC classification number: G01N21/65 ,  G01N21/84 ,  G01N33/0098 ,  G01N2021/8466

Abstract: The present invention relates to the use of a Raman spectral signature for detection of plant metabolites, specifically carotenoids, in tissue of a plant leaf. Carotenoids are used as a biomarker for an early, real-time diagnosis of shade avoidance syndrome (SAS) in growing plants in a non-invasive or non-destructive way in order to detect the adverse effect of the SAS upon their health, and ultimately their yield. The early, real-time diagnosis of SAS provides a window period within which further adverse effects of SAS may be slowed or prevented without negatively affecting the yield of growing plants or leafy vegetables.

公开(公告)号：US20210123066A1

公开(公告)日：2021-04-29

申请号：US16963133

申请日：2019-01-17

Applicant: TEMASEK LIFE SCIENCES LABORATORY LIMITED

Inventor： In-Cheol JANG ,  Jun-shi ZHENG ,  Mi Jung KIM

IPC: C12N15/82 ,  A01H4/00

Abstract: The present invention relates to a method for Agrobacterium-mediated transformation and regeneration of Stevia plants. In particular, the method involves co-culturing leaf explants with Agrobacterium in a medium comprising acetosyringone and 2,4-dichlorophenoxyacetic acid in the dark, callus induction and shoot regeneration in a medium comprising 6-benzylaminopurine, 3-indoleacetic acid, a selective agent and an Agrobacterium eradicant in the dark, and root regeneration in a medium comprising 3-indoleacetic acid in a light/dark cycle. The present invention also relates to the overexpression of SrDXS1 and SrKAH in transgenic plants, resulting in the enhancement of steviol glycosides in the transgenic plants. The present invention further relates to the overexpression SrUGT76G1 in transgenic plants, resulting in higher Rebaudioside A (Reb A) to stevioside ratios in the transgenic plants.

公开(公告)号：US20220098601A2

公开(公告)日：2022-03-31

申请号：US16963133

申请日：2019-01-17

Applicant: TEMASEK LIFE SCIENCES LABORATORY LIMITED

Inventor： In-Cheol JANG ,  Jun-shi ZHENG ,  Mi Jung KIM

IPC: C12N15/82 ,  A01H4/00

Abstract: The present invention relates to a method for Agrobacterium-mediated transformation and regeneration of Stevia plants. In particular, the method involves co-culturing leaf explants with Agrobacterium in a medium comprising acetosyringone and 2,4-dichlorophenoxyacetic acid in the dark, callus induction and shoot regeneration in a medium comprising 6-benzylaminopurine, 3-indoleacetic acid, a selective agent and an Agrobacterium eradicant in the dark, and root regeneration in a medium comprising 3-in-doleacetic acid in a light/dark cycle. The present invention also relates to the overexpression of SrDXS I and SrKAH in transgenic plants, resulting in the enhancement of steviol glycosides in the transgenic plants. The present invention further relates to the overexpression SrUGT76G I in transgenic plants, resulting in higher Rebaudioside A (Reb A) to stevioside ratios in the transgenic plants.