公开(公告)号：US07683345B2

公开(公告)日：2010-03-23

申请号：US12185785

申请日：2008-08-04

申请人： Paul C. Goodwin ,  Carl S. Brown ,  Steven A. Reese

发明人： Paul C. Goodwin ,  Carl S. Brown ,  Steven A. Reese

IPC分类号： G01J1/58

CPC分类号： G01N33/582 ,  G01N15/1475 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/6458 ,  G01N33/5005 ,  G06T5/003 ,  G06T2207/10064 ,  G06T2207/30024

摘要： A system and method employing photokinetic techniques in cell biology imaging applications are disclosed. Systems and methods of acquiring image data of an object may comprise: selectively inducing photoactivation of material at a site on the object; performing an optical axis integration scan; simultaneously executing a time delay integration scan sequence; and processing acquired image data in accordance with one or more desired analyses. Various methodologies and applications may include, inter alia, selective photobleaching of a site on the object, diffusion rate, velocity, and wave-front propagation analyses, multi-dimensional analyses of dispersion characteristics, biomolecular binding in cellular organelles, and photoactivation assisted systematic image segmentation for the study of cellular components.

摘要翻译： 公开了在细胞生物学成像应用中使用光动力学技术的系统和方法。 获取对象的图像数据的系统和方法可以包括：选择性地诱导物体上的物体的材料的光活化; 执行光轴积分扫描; 同时执行时间延迟积分扫描序列; 以及根据一个或多个期望的分析处理获取的图像数据。 各种方法和应用可以包括对物体上的位点的选择性漂白，扩散速率，速度和波前传播分析，分散特征的多维分析，细胞器中的生物分子结合和光激活辅助系统图像 细胞分析研究的细分。

公开(公告)号：US20090026386A1

公开(公告)日：2009-01-29

申请号：US12185775

申请日：2008-08-04

申请人： Paul C. Goodwin ,  Carl S. Brown ,  Steven A. Reese

发明人： Paul C. Goodwin ,  Carl S. Brown ,  Steven A. Reese

IPC分类号： G01J1/58

CPC分类号： G01N33/582 ,  G01N15/1475 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/6458 ,  G01N33/5005 ,  G06T5/003 ,  G06T2207/10064 ,  G06T2207/30024

摘要： A system and method employing photokinetic techniques in cell biology imaging applications are disclosed. Systems and methods of acquiring image data of an object may comprise: selectively inducing photoactivation of material at a site on the object; performing an optical axis integration scan; simultaneously executing a time delay integration scan sequence; and processing acquired image data in accordance with one or more desired analyses. Various methodologies and applications may include, inter alia, selective photobleaching of a site on the object, diffusion rate, velocity, and wave-front propagation analyses, multi-dimensional analyses of dispersion characteristics, biomolecular binding in cellular organelles, and photoactivation assisted systematic image segmentation for the study of cellular components.

摘要翻译： 公开了在细胞生物学成像应用中使用光动力学技术的系统和方法。 获取对象的图像数据的系统和方法可以包括：选择性地诱导物体上的物体的材料的光活化; 执行光轴积分扫描; 同时执行时间延迟积分扫描序列; 以及根据一个或多个期望的分析处理获取的图像数据。 各种方法和应用可以包括对物体上的位点的选择性漂白，扩散速率，速度和波前传播分析，分散特征的多维分析，细胞器中的生物分子结合和光活化辅助系统图像 细胞分析研究的细分。

公开(公告)号：US07608840B2

公开(公告)日：2009-10-27

申请号：US12185775

申请日：2008-08-04

申请人： Paul C. Goodwin ,  Carl S. Brown ,  Steven A. Reese

发明人： Paul C. Goodwin ,  Carl S. Brown ,  Steven A. Reese

IPC分类号： G01N21/64

CPC分类号： G01N33/582 ,  G01N15/1475 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/6458 ,  G01N33/5005 ,  G06T5/003 ,  G06T2207/10064 ,  G06T2207/30024

摘要： A system and method employing photokinetic techniques in cell biology imaging applications are disclosed. Systems and methods of acquiring image data of an object may comprise: selectively inducing photoactivation of material at a site on the object; performing an optical axis integration scan; simultaneously executing a time delay integration scan sequence; and processing acquired image data in accordance with one or more desired analyses. Various methodologies and applications may include, inter alia, selective photobleaching of a site on the object, diffusion rate, velocity, and wave-front propagation analyses, multi-dimensional analyses of dispersion characteristics, biomolecular binding in cellular organelles, and photoactivation assisted systematic image segmentation for the study of cellular components.

摘要翻译： 公开了在细胞生物学成像应用中使用光动力学技术的系统和方法。 获取物体的图像数据的系统和方法可以包括：选择性地诱导物体上的物体的材料的光活化; 执行光轴积分扫描; 同时执行时间延迟积分扫描序列; 以及根据一个或多个期望的分析处理获取的图像数据。 各种方法和应用可以包括对物体上的位点的选择性漂白，扩散速率，速度和波前传播分析，分散特征的多维分析，细胞器中的生物分子结合和光活化辅助系统图像 细胞分析研究的细分。

公开(公告)号：US07283253B2

公开(公告)日：2007-10-16

申请号：US10389269

申请日：2003-03-13

申请人： Steven A. Reese ,  Carl S. Brown ,  Paul C. Goodwin

发明人： Steven A. Reese ,  Carl S. Brown ,  Paul C. Goodwin

IPC分类号： G01B11/24

CPC分类号： G02B21/367 ,  G06T1/0007 ,  H04N5/349 ,  H04N5/37206 ,  H04N5/3743

摘要： An image acquisition system and method employing multi-axis integration (MAI) may incorporate both optical axis integration (OAI) and time-delay integration (TDI) techniques. Disclosed MAI systems and methods may integrate image data in the z direction as the data are acquired, projecting the image data prior to deconvolution. Lateral translation of the image plane during the scan in the z direction may allow large areas to be imaged in a single scan sequence.

摘要翻译： 使用多轴积分（MAI）的图像采集系统和方法可以并入光轴积分（OAI）和时延整合（TDI）技术。 所公开的MAI系统和方法可以在采集数据时在z方向上集成图像数据，在去卷积之前投影图像数据。 在z方向扫描期间的图像平面的横向平移可允许以单个扫描序列成像大面积。

公开(公告)号：US07408176B2

公开(公告)日：2008-08-05

申请号：US10872329

申请日：2004-06-18

申请人： Paul C. Goodwin ,  Carl S. Brown ,  Steven A. Reese

发明人： Paul C. Goodwin ,  Carl S. Brown ,  Steven A. Reese

IPC分类号： G01N21/64

CPC分类号： G01N33/582 ,  G01N15/1475 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/6458 ,  G01N33/5005 ,  G06T5/003 ,  G06T2207/10064 ,  G06T2207/30024

摘要： A system and method employing photokinetic techniques in cell biology imaging applications are disclosed. Systems and methods of acquiring image data of an object may comprise: selectively inducing photoactivation of material at a site on the object; performing an optical axis integration scan; simultaneously executing a time delay integration scan sequence; and processing acquired image data in accordance with one or more desired analyses. Various methodologies and applications may include, inter alia, selective photobleaching of a site on the object, diffusion rate, velocity, and wave-front propagation analyses, multi-dimensional analyses of dispersion characteristics, biomolecular binding in cellular organelles, and photoactivation assisted systematic image segmentation for the study of cellular components.

摘要翻译： 公开了在细胞生物学成像应用中使用光动力学技术的系统和方法。 获取对象的图像数据的系统和方法可以包括：选择性地诱导物体上的物体的材料的光活化; 执行光轴积分扫描; 同时执行时间延迟积分扫描序列; 以及根据一个或多个期望的分析处理获取的图像数据。 各种方法和应用可以包括对物体上的位点的选择性漂白，扩散速率，速度和波前传播分析，分散特征的多维分析，细胞器中的生物分子结合和光激活辅助系统图像 细胞分析研究的细分。

公开(公告)号：US20090028413A1

公开(公告)日：2009-01-29

申请号：US12185785

申请日：2008-08-04

申请人： Paul C. Goodwin ,  Carl S. Brown ,  Steven A. Reese

发明人： Paul C. Goodwin ,  Carl S. Brown ,  Steven A. Reese

IPC分类号： G06K9/54 ,  C12Q1/02

CPC分类号： G01N33/582 ,  G01N15/1475 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/6458 ,  G01N33/5005 ,  G06T5/003 ,  G06T2207/10064 ,  G06T2207/30024

摘要： A system and method employing photokinetic techniques in cell biology imaging applications are disclosed. Systems and methods of acquiring image data of an object may comprise: selectively inducing photoactivation of material at a site on the object; performing an optical axis integration scan; simultaneously executing a time delay integration scan sequence; and processing acquired image data in accordance with one or more desired analyses. Various methodologies and applications may include, inter alia, selective photobleaching of a site on the object, diffusion rate, velocity, and wave-front propagation analyses, multi-dimensional analyses of dispersion characteristics, biomolecular binding in cellular organelles, and photoactivation assisted systematic image segmentation for the study of cellular components.

公开(公告)号：US20090109416A1

公开(公告)日：2009-04-30

申请号：US12208660

申请日：2008-09-11

申请人： WILLIAM M. DOUGHERTY ,  Paul C. Goodwin ,  Steven A. Reese ,  David A. Stewart

发明人： WILLIAM M. DOUGHERTY ,  Paul C. Goodwin ,  Steven A. Reese ,  David A. Stewart

IPC分类号： G03B27/54 ,  G02B3/12

CPC分类号： G02B21/33 ,  G02B21/002 ,  G02B21/02 ,  G03F7/70341

摘要： Methods and apparatus are described for delivering index-matching immersion liquid in high numerical-aperture optical microscopy and lithography. An array of immersion liquid droplets is delivered to a specimen substrate or specimen substrate cover by an immersion liquid printing apparatus. An immersion liquid reservoir provides immersion liquid to the printer by a precision pump. The printer delivers immersion liquid to the substrate or substrate cover in arrays of immersion liquid droplets of defined volumes and array patterns. The volumes and patterns of array droplets delivered to the substrate or substrate cover are optimized to maintain adequate immersion liquid between the substrate or substrate cover and an immersion objective while avoiding the formation of air bubbles in the immersion liquid and the accumulation of excess volumes of immersion liquid.

摘要翻译： 描述了用于在高数值孔径光学显微镜和光刻中输送折射率匹配浸液的方法和装置。 浸没液体液滴的阵列通过浸没液体印刷装置输送到样品基片或样品基片盖。 浸没液体储存器通过精密泵向打印机提供浸液。 打印机将浸没液体以定义的体积和阵列图案的浸没液滴的阵列传送到基底或基底盖。 优化输送到衬底或衬底盖的阵列液滴的体积和图案，以在衬底或衬底盖和浸没物体之间保持足够的浸没液体，同时避免在浸液中形成气泡和积累过量的浸液 液体。

公开(公告)号：US20090206238A1

公开(公告)日：2009-08-20

申请号：US12346392

申请日：2008-12-30

申请人： Carl S. Brown ,  Steven A. Reese

发明人： Carl S. Brown ,  Steven A. Reese

IPC分类号： H01J3/14 ,  G01J1/58

CPC分类号： G02B21/16 ,  G02B21/0076 ,  G02B21/34

摘要： A new optical substrate design allows a target to be illuminated with minimal illumination of undesired surfaces within the image collection ray path. The non rectangular substrate provide different surfaces through which a target is illuminated and imaged and thereby prevents illumination rays from crossing the substrate surface through which the target is imaged

摘要翻译： 新的光学基板设计允许以图像收集射线路径内的不需要的表面的最小照度照亮目标。 非矩形基板提供不同的表面，通过该不同的表面，靶被照射和成像，从而防止照射光线穿过靶被成像的基板表面

公开(公告)号：US08115150B2

公开(公告)日：2012-02-14

申请号：US12346392

申请日：2008-12-30

申请人： Carl S. Brown ,  Steven A. Reese

发明人： Carl S. Brown ,  Steven A. Reese

IPC分类号： H01J3/14

CPC分类号： G02B21/16 ,  G02B21/0076 ,  G02B21/34

摘要： A new optical substrate design allows a target to be illuminated with minimal illumination of undesired surfaces within the image collection ray path. The non rectangular substrate provide different surfaces through which a target is illuminated and imaged and thereby prevents illumination rays from crossing the substrate surface through which the target is imaged

摘要翻译： 新的光学基板设计允许以图像收集射线路径内的不需要的表面的最小照度照亮目标。 非矩形基板提供不同的表面，通过该不同的表面，靶被照射和成像，从而防止照射光线穿过靶被成像的基板表面

公开(公告)号：US09256066B2

公开(公告)日：2016-02-09

申请号：US14002615

申请日：2012-01-16

申请人： Jeremy R. Cooper ,  Steven A. Reese ,  William M. Dougherty

发明人： Jeremy R. Cooper ,  Steven A. Reese ,  William M. Dougherty

IPC分类号： G02B26/08 ,  G02B26/10 ,  G02B26/12 ,  G02B21/16 ,  G02B6/26 ,  G02B6/32

CPC分类号： G02B26/08 ,  G02B6/266 ,  G02B6/32 ,  G02B21/16 ,  G02B26/0816 ,  G02B26/0875 ,  G02B26/105 ,  G02B2207/113

摘要： Irradiance control systems (“ICSs”) that control the irradiance of a beam of light are disclosed. ICSs include in a beam translator and a beam launch. The beam translator translates the beam substantially perpendicular to the propagating direction of the beam with a desired displacement so that the beam launch can remove a portion of the translated beam and the beam can be output with a desired irradiance. The beam launch attenuates the irradiance of the beam based on the amount by which the beam is translated. ISCs can be incorporated into fluorescent microscopy instruments to provide high-speed, fine-tune control over the irradiance of excitation beams.

摘要翻译： 公开了控制光束辐照度的照度控制系统（“ICS”）。 ICS包括光束转换器和光束发射。 光束平移器将光束基本上垂直于光束的传播方向以期望的位移平移，使得光束发射可以去除平移光束的一部分，并且可以以期望的辐照度输出光束。 光束发射基于光束被平移的量来衰减光束的辐照度。 ISCs可以并入荧光显微镜仪器，以提供对激发光束的辐照度的高速，微调控制。