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13 条记录 (耗时 0.015 秒)

    Modified Fibroin
    1.
    发明申请
    Modified Fibroin 审中-公开

    公开(公告)号：US20200207817A1

    公开(公告)日：2020-07-02

    申请号：US16633589

    申请日：2018-07-25

    申请人： Spiber Inc. ,  Riken

    发明人： Keisuke Morita ,  Yunosuke Abe ,  Takehisa Maekawa ,  Koichi Kotaka ,  Junichi Sugahara ,  Keiji Numata

    IPC分类号： C07K14/435 ,  D01F4/02 ,  C12N15/63

    摘要： The present invention relates to a modified fibroin including a domain sequence represented by Formula 1: [(A)n motif-REP]m or Formula 2: [(A)n motif-REP]m-(A)n motif, in which the domain sequence has an amino acid sequence with a reduced content of a glutamine residue equivalent to an amino acid in which one or a plurality of glutamine residues in REP are deleted or substituted with other amino acid residues, as compared with a naturally occurring fibroin. [In Formula 1 and Formula 2, (A)n motif represents an amino acid sequence consisting of 4 to 27 amino acid residues, and the number of alanine residues with respect to the total number of amino acid residues in the (A)n motif is 80% or more. REP represents an amino acid sequence consisting of 10 to 200 amino acid residues. m represents an integer of 10 to 300. A plurality of (A)n motifs may be the same amino acid sequence or different amino acid sequences.]

    Production method for insoluble recombinant protein aggregate
    4.
    发明授权
    Production method for insoluble recombinant protein aggregate 有权

    公开(公告)号：US10899792B2

    公开(公告)日：2021-01-26

    申请号：US16324280

    申请日：2017-08-10

    申请人： Spiber Inc.

    发明人： Toshiaki Osawa ,  Yuya Sato ,  Keisuke Morita

    IPC分类号： C07K1/34 ,  C07K1/30 ,  C12P21/02 ,  C12P21/00 ,  C07K1/14

    摘要： An object of the present invention is to provide a method for efficiently separating insoluble bodies of a recombinant protein from a recombinant cell expressing a target recombinant protein as insoluble bodies in the cell. The present invention provides a method for producing a recombinant protein aggregate by separating insoluble bodies of a recombinant protein from a recombinant cell expressing the recombinant protein as insoluble bodies in the cell, including disrupting the recombinant cell, aggregating the insoluble bodies of the recombinant protein, and separating the resulting aggregate.

    Modified Fibroin
    8.
    发明申请
    Modified Fibroin 审中-公开

    公开(公告)号：US20190135881A1

    公开(公告)日：2019-05-09

    申请号：US16096824

    申请日：2017-04-28

    申请人： Spiber Inc. ,  Kojima Industries Corporation

    发明人： Keisuke Morita ,  Junichi Sugahara

    IPC分类号： C07K14/435 ,  D01F4/02

    摘要： Provided is a modified fibroin, including a domain sequence represented by Formula 1: [(A)n motif-REP]m, in which the domain sequence has an amino acid sequence having a reduced content of glycine residues equivalent to an amino acid sequence in which, at least, one or a plurality of the glycine residues in REP is substituted with another amino acid residue, as compared to naturally occurring fibroin:[In Formula 1, (A)n motif represents an amino acid sequence consisting of 4 to 20 amino acid residues and the number of alanine residues relative to the total number of amino acid residues in the (A)n motif is 83% or more, REP represents an amino acid sequence consisting of 10 to 200 amino acid residues, m represents an integer of 8 to 300, and a plurality of (A)n motifs or the like may be the same amino acid sequence or different amino acid sequences.]