-
公开(公告)号：US10907179B2
公开(公告)日：2021-02-02
申请号：US15579434
申请日：2016-06-28
发明人： Haruhiko Yamaguchi , Yukino Inoue , Kazuhisa Fushihara , Seiji Takahashi , Satoshi Yamashita , Toru Nakayama
IPC分类号： C12P5/00 , C12P5/02 , B60C1/00 , C12N15/09 , C07K14/415 , C12N15/82 , C12N9/10 , A01H5/02 , C08F136/08 , C08L47/00
摘要： The present invention provides a method for producing a polyisoprenoid with which it is possible to enhance the rubber synthesis activity of rubber particles to increase natural rubber production. The present invention relates to a method for producing a polyisoprenoid in vitro, which involves the use of a gene coding for a cis-prenyltransferase (CPT) family protein and a gene coding for a Nogo-B receptor (NgBR) family protein, and further involves the use of rubber particles bound to proteins encoded by these genes; or a method for producing a polyisoprenoid, which includes introducing into a plant a vector in which a promoter having a promoter activity that drives laticifer-specific gene expression is linked to a gene coding for a CPT family protein and a gene coding for a NgBR family protein, to express proteins encoded by the genes specifically in laticifers.
-
公开(公告)号：US11236365B2
公开(公告)日：2022-02-01
申请号：US16502750
申请日：2019-07-03
发明人： Yukino Inoue , Haruhiko Yamaguchi , Kazuhisa Fushihara , Seiji Takahashi , Satoshi Yamashita , Toru Nakayama
IPC分类号： C12N15/82 , C12P5/00 , C12N9/10 , B29D30/08 , B29D30/06 , C07K14/415 , B29K607/00
摘要： Provided is a method for producing a polyisoprenoid, which can increase natural rubber production by enhancing the rubber synthesis activity of rubber particles. The present invention provides methods for producing a polyisoprenoid using a gene coding for a cis-prenyltransferase (CPT) family protein, a gene coding for a Nogo-B receptor (NgBR) family protein and a gene coding for a rubber elongation factor (REF) family protein, specifically a method for producing a polyisoprenoid in vitro using rubber particles bound to proteins coded for by these genes, and a method for producing a polyisoprenoid in vivo using a recombinant organism (plant) having these genes introduced therein.
-
公开(公告)号：US09777077B2
公开(公告)日：2017-10-03
申请号：US15106560
申请日：2014-12-16
CPC分类号： C08C1/10 , C08C1/06 , C08J3/05 , C08J2307/02 , C08L7/02
摘要： The present invention provides an effective method for fractionating rubber particles in natural rubber latex by particle size, and effective methods for transporting or preserving rubber particles. The present invention relates to a method for fractionating rubber particles in natural rubber latex by particle size, the method including centrifuging natural rubber latex in at least four stages at forces of 800 to 3,000×g, 6,500 to 10,000×g, 17,000 to 22,000×g, and 40,000 to 60,000×g to fractionate rubber particles. The present invention also relates to methods for transporting or preserving rubber particles, the methods including the steps of adding a buffer solution and an antioxidant to natural rubber latex or rubber particles fractionated from natural rubber latex; and freezing a mixture prepared in the above step.
-
公开(公告)号：US10889623B2
公开(公告)日：2021-01-12
申请号：US15579499
申请日：2016-05-31
发明人： Haruhiko Yamaguchi , Yukino Inoue , Kazuhisa Fushihara , Seiji Takahashi , Satoshi Yamashita , Toru Nakayama , Yuzuru Tozawa
IPC分类号： C07K14/415 , B60C1/00 , C08G81/02 , B29D30/00 , C12N15/09 , B29D30/06 , C12N15/62 , C08H1/00
摘要： The present invention provides a method for producing rubber particles bound to a membrane-associated protein by cell-free protein synthesis. The present invention relates to a method for producing rubber particles bound to a membrane-associated protein, the method including the step of performing protein synthesis in the presence of both rubber particles and a cell-free protein synthesis solution containing an mRNA coding for a membrane-associated protein to bind the membrane-associated protein to the rubber particles.
-
公开(公告)号：US10385362B2
公开(公告)日：2019-08-20
申请号：US15170584
申请日：2016-06-01
发明人： Yukino Inoue , Haruhiko Yamaguchi , Kazuhisa Fushihara , Seiji Takahashi , Satoshi Yamashita , Toru Nakayama
IPC分类号： C12N15/82 , C12P5/00 , C12N9/10 , B29D30/08 , B29D30/06 , C07K14/415 , B29K607/00
摘要： Provided is a method for producing a polyisoprenoid, which can increase natural rubber production by enhancing the rubber synthesis activity of rubber particles. The present invention provides methods for producing a polyisoprenoid using a gene coding for a cis-prenyltransferase (CPT) family protein, a gene coding for a Nogo-B receptor (NgBR) family protein and a gene coding for a rubber elongation factor (REF) family protein, specifically a method for producing a polyisoprenoid in vitro using rubber particles bound to proteins coded for by these genes, and a method for producing a polyisoprenoid in vivo using a recombinant organism (plant) having these genes introduced therein.
-
公开(公告)号：US10059780B2
公开(公告)日：2018-08-28
申请号：US15170278
申请日：2016-06-01
发明人： Haruhiko Yamaguchi , Yukino Inoue , Kazuhisa Fushihara , Seiji Takahashi , Satoshi Yamashita , Toru Nakayama
IPC分类号： C08C19/22 , C07K14/415
CPC分类号： C08C19/22 , C07K14/415
摘要： Provided is a method for producing rubber particles with a reduced coagulation tendency. The present invention relates to a method for producing rubber particles with a reduced coagulation tendency, the method including a step of performing protein synthesis in the presence of both rubber particles and a cell-free protein synthesis solution containing an mRNA coding for a rubber elongation factor (REF) family protein to bind the REF family protein to the rubber particles.
-
公开(公告)号：US10420290B2
公开(公告)日：2019-09-24
申请号：US15408744
申请日：2017-01-18
发明人： Haruhiko Yamaguchi
摘要： A method for cultivating plants of the Asteraceae family, which promotes flower bud formation in the reproductive phase. A method for cultivating plants of the Asteraceae family, wherein the cultivation temperature in the reproductive phase is set lower by at least 5° C. than the cultivation temperature in the vegetative phase to promote flower bud formation in the reproductive phase.
-
公开(公告)号：US10000774B2
公开(公告)日：2018-06-19
申请号：US15107178
申请日：2015-01-06
发明人： Haruhiko Yamaguchi
IPC分类号： C12N5/04 , C12N1/20 , C12N15/00 , C12N9/10 , C12P5/00 , C07H21/04 , C07H21/02 , C07K14/00 , C12N15/82 , C07K14/705 , C07K14/415
CPC分类号： C12P5/007 , C07K14/415 , C07K14/705 , C12N9/1085 , C12N15/8201 , C12Y205/0102
摘要： Provided are a transformant produced by introducing a gene coding for a cis-prenyltransferase and a gene coding for a Nogo-B receptor, which are considered to be involved in polyisoprenoid biosynthesis, into a host to allow the host to express the cis-prenyltransferase and the Nogo-B receptor, and a method for producing a polyisoprenoid using the transformant. The present invention relates to a transformant produced by introducing a gene coding for a cis-prenyltransferase and a gene coding for a Nogo-B receptor into a host to allow the host to express the cis-prenyltransferase and the Nogo-B receptor.
-
公开(公告)号：US10920234B2
公开(公告)日：2021-02-16
申请号：US16435010
申请日：2019-06-07
IPC分类号： C12N15/82
摘要： Provided is a method that can efficiently produce a transgenic Taraxacum plant in a short period. A method of producing a transgenic Taraxacum plant, including: an infection step of infecting a tissue fragment from a Taraxacum plant with an Agrobacterium tumefaciens containing a plasmid containing a target gene or fragment thereof and hygromycin-resistance gene; a selective culture step of selecting the tissue fragment that has acquired the target gene from the tissue fragment obtained in the infection step by using hygromycin; a callus-inducing step of culturing the tissue fragment obtained in the selective culture step in a callus-inducing medium to form callus; a regeneration-inducing step of culturing the callus obtained in the callus-inducing step in a regeneration-inducing medium to form an adventitious embryo, an adventitious bud, and a shoot; and a rooting step of culturing the shoot obtained in the regeneration-inducing step in a rooting medium to root the shoot.
-
公开(公告)号：US10000763B2
公开(公告)日：2018-06-19
申请号：US14429606
申请日：2013-10-01
发明人： Haruhiko Yamaguchi , Yukino Inoue , Satoshi Kuroda
IPC分类号： C12N15/82 , C12P5/00 , C12P5/02 , C07K14/415
CPC分类号： C12N15/8243 , C07K14/415 , C12P5/007 , C12P5/02
摘要： The present invention provides a method for enhancing the overall pathway of polyisoprenoid biosynthesis. The present invention further provides an isoprenoid-producing plant having an overall enhanced pathway of polyisoprenoid biosynthesis, and a method of producing a polyisoprenoid using such an isoprenoid-producing plant. The present invention relates to a method of regulating by a cytokinin-responsive transcription factor the expression of at least one protein selected from the group consisting of hydroxymethylglutaryl-CoA reductase, isopentenyl diphosphate isomerase, cis-prenyltransferase, and small rubber particle protein.
-
-
-
-
-
-
-
-
-
搜索结果
19 条记录 (耗时 0.023 秒)
