公开(公告)号：US20240108784A1

公开(公告)日：2024-04-04

申请号：US18081964

申请日：2022-12-15

Applicant: SHANDONG UNIVERSITY ,  YANSHAN UNIVERSITY

Inventor： Chuanzhen HUANG ,  Zhuang CHEN ,  Hanlian LIU ,  Peng YAO ,  Zhenyu SHI ,  Dun LIU ,  Hongtao ZHU ,  Bin ZOU ,  Zhen WANG ,  Minting WANG ,  Longhua XU ,  Shuiquan HUANG ,  Meina QU ,  Zhengkai XU ,  Yabin GUAN

IPC: A61L26/00 ,  B29C64/118 ,  B33Y10/00 ,  B33Y70/00 ,  B33Y80/00

CPC classification number: A61L26/008 ,  A61L26/0052 ,  B29C64/118 ,  B33Y10/00 ,  B33Y70/00 ,  B33Y80/00 ,  B29K2005/00 ,  B29L2031/753

Abstract: A hydrogel for cell-laden bioprinting, bioink, and a preparation method and an application thereof, relates to the technical field of biomedical polymer hydrogels. The hydrogel for cell-laden bioprinting is polymer gel formed by adding a cell-specific material into a matrix of alginate and gelatin and crosslinking and curing, wherein the cell-specific material is polypeptide selected according to different laden cells. The structures printed using the hydrogel may have the advantages such as adjustable mechanical properties, adjustable porosity, high biocompatibility, high printing accuracy, and high customizability, which may widely support the printing of human tissues and organs such as spinal cord, cartilage, and heart, and has good prospects for applications in tissue repair, organ transplantation and so on.

公开(公告)号：US20240302352A1

公开(公告)日：2024-09-12

申请号：US18182091

申请日：2023-03-10

Applicant: SHANDONG UNIVERSITY ,  YANSHAN UNIVERSITY

Inventor： Chuanzhen HUANG ,  Zhuang CHEN ,  Hanlian LIU ,  Peng YAO ,  Dun LIU ,  Hongtao ZHU ,  Bin ZOU ,  Zhen WANG ,  Jun WANG ,  Longhua XU ,  Shuiquan HUANG ,  Meina QU ,  Zhengkai XU ,  Minting WANG ,  Yabin GUAN

IPC: G01N33/50 ,  B33Y70/00 ,  B33Y80/00 ,  C12N5/079 ,  C12N5/0793 ,  C12N5/0797

CPC classification number: G01N33/5082 ,  B33Y70/00 ,  B33Y80/00 ,  C12N5/0618 ,  C12N5/0619 ,  C12N5/0623 ,  C12N2501/13 ,  C12N2513/00 ,  C12N2533/54 ,  C12N2533/74

Abstract: A method of rapid constructing human cerebral cortical organoids by 3D bioprinting and an application including preparing microfluidic chips, preparation of hydrogel of human cerebral cortical organoids, and printing of human cerebral cortical organoids. The microfluidic chip comprises a mixed-flow channel layer, liquid pool layer, microporous array layer, human cerebral cortical organoid culture layer, and culture medium recovery layer; the human cerebral cortical organoid hydrogel has gelatin, alginate, and hyaluronic acid; printing directly human cerebral cortical organoids in microfluidic chips by FRESH printing method, obtaining human cerebral cortical organoid chips after packaging. The application directly constructs large-scale human cerebral cortex-like with three layers of mutually connected structures in situ in organ chip through 3D bioprinting, simulates cerebrospinal fluid circulation through perfusion culture.