公开(公告)号：US20250035618A1

公开(公告)日：2025-01-30

申请号：US18709250

申请日：2022-12-27

Applicant: SEKISUI MEDICAL CO., LTD.

Inventor： Daisuke YAMADA ,  Ikumi SUZUKI ,  Kengo FUJIMURA ,  Tomohide ASAI ,  Osamu MIYAZAKI

IPC: G01N33/543 ,  G01N33/53 ,  G01N33/536

Abstract: It is an object of the present invention to provide a method for suppressing non-specific reactions using a highly versatile method without affecting specific reactions. The present invention provides a detection method for detecting a target antigen in a sample using a specific antibody that specifically binds to the antigen, the method comprising: a) a step of bringing the sample into contact with a modified antibody; and b) a step of bringing the sample into contact with the specific antibody simultaneously with or after the step a), which is a highly versatile method that can suppress non-specific reactions.

公开(公告)号：US20220267852A1

公开(公告)日：2022-08-25

申请号：US17630069

申请日：2020-07-22

Applicant: SEKISUI MEDICAL CO., LTD.

Inventor： Hiroyuki EBINUMA ,  Ikumi SUZUKI

IPC: C12Q1/6883

Abstract: An object of the invention is to provide a primer for detecting and quantifying homozygous deletion of the SMN1 gene, the deletion of which causes SMA, using a dried blood spot in a filter paper and a method related to specific detection/quantification of the SMN1 gene using the primer. The invention is a method for detecting the SMN1 gene in a dried blood spot in a filter paper by real-time PCR, including the steps of (A) to (D) below: (A) a step of adding the dried blood spot in a filter paper to a PCR reaction tube; (B) a step of adding a PCR reagent to the PCR reaction tube, wherein the PCR reagent contains at least a primer designed in a manner that the reactivity to the SMN2 gene is less than 1% of that to the SMN1 gene, a polymerase, dNTPs and an intercalator or a fluorescently labeled probe; (C) a step of performing PCR reaction in the tube containing the PCR reagent and the dried blood spot in a filter paper; and (D) a step of sequentially and optically detecting a target nucleic acid in the SMN1 gene amplified by the PCR reaction.

公开(公告)号：US20230090926A1

公开(公告)日：2023-03-23

申请号：US17801212

申请日：2021-02-19

Applicant: SEKISUI MEDICAL CO., LTD.

Inventor： Hiroyuki EBINUMA ,  Ikumi SUZUKI

IPC: C12Q1/686 ,  C12Q1/6876

Abstract: It is an object of the present disclosure to provide a method for reducing the influence of baseline disturbances in a method for detecting a target nucleic acid in dry blood filter paper by real-time PCR using a fluorescent dye, with a simple method. The present disclosure provides a method for detecting a target nucleic acid in dry blood filter paper by real-time PCR, the method including: (1) amplifying the target nucleic acid in the dry blood filter paper by applying thermal cycles to a sample solution containing a dry blood filter paper punch piece and a PCR reagent, wherein the PCR reagent includes a fluorescently labeled probe; (2) optically detecting the fluorescence intensity of the sample solution for each of the thermal cycles; and (3) performing quantitative analysis of the target nucleic acid using data after a predetermined number of cycles of the optically detected data.

公开(公告)号：US20230025583A1

公开(公告)日：2023-01-26

申请号：US17780846

申请日：2020-11-27

Applicant: SEKISUI MEDICAL CO., LTD.

Inventor： Tomohide ASAI ,  Yoshihiro TANAKA ,  Ikumi SUZUKI ,  Satoko SEO ,  Tomoyuki USUI ,  Tomo SHIMIZU ,  Osamu MIYAZAKI

IPC: G01N33/543 ,  C07K16/18

Abstract: A problem to be solved by the invention is to improve the sensitivity of measurement of an analyte in a sample by a sandwich immunoassay. The sensitivity can be improved significantly in a sandwich immunoassay using a first antibody and a second antibody when one or both of the first antibody and the second antibody are a mixture of a monoclonal antibody recognizing a linear epitope and a monoclonal antibody recognizing a conformational epitope.