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公开(公告)号：US20230184641A1
公开(公告)日：2023-06-15
申请号：US17926085
申请日：2021-05-18
申请人： S2 Genomics, Inc.
CPC分类号： G01N1/36 , B01L7/52 , B01L3/502715 , G01N2001/2866
摘要： A system, methods, and apparatus are described to collect and prepare cells, nuclei, subcellular components, and biomolecules from specimens including FFPE and OCT preserved tissues. The system can perform deparaffinization, rehydration, enzymatic and/or chemical and physical disruption of the FFPE tissue, or residue removal of the OCT tissue, to dissociate it into a single cell or nuclei suspension.
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公开(公告)号：US11231347B2
公开(公告)日：2022-01-25
申请号：US16301249
申请日：2017-11-29
申请人： S2 Genomics, Inc. , Stevan Jovanovich , Frank Zaugg , Kaiwan Chear , Roger McIntosh , Nathan Pereira
发明人： Stevan Jovanovich , Frank Zaugg , Kaiwan Chear , Roger McIntosh , Nathan Pereira
摘要： A system, methods, and apparatus are described to collect and prepare single cells, nuclei, subcellular components, and biomolecules from specimens including tissues. The system can perform enzymatic and/or physical disruption of the tissue to dissociate it into single-cells or nuclei in suspension or subcellular components including nucleic acids. In some embodiments, the titer of dissociated cells is monitored at intervals and the viability determined. In some embodiments, the processing is adjusted according to the measurements of the titer and viability. In some embodiments, the single-cells or nuclei in suspension are washed and resuspended in the buffer or media of choice. In some embodiments, the conditions are chosen to produce nuclei. In other embodiments, the single-cells or nuclei are purified by affinity paramagnetic bead processing. In some embodiments, matched bulk nucleic acid to the single-cells is produced. In other embodiments, single-cell libraries, or nuclei libraries, or matched bulk libraries, or bulk libraries are produced. The single cells or nuclei can then be further processed by FACS, DNA sequencing, mass spectrometry, fluorescence, or other methods. In other embodiments, the tissue processing is integrated with an analytical system to produce a sample-to-answer system such as a tissue-to-genomics system.
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公开(公告)号：US20240027311A1
公开(公告)日：2024-01-25
申请号：US18229141
申请日：2023-08-01
申请人： S2 Genomics, Inc.
CPC分类号： G01N1/31 , C12M47/04 , C12M47/06 , C12Q1/6851 , C12N1/066 , C12N15/1003 , C12N1/06 , G01N1/286 , G01N2001/2866
摘要： This disclosure provides methods for producing a sample of subcellular organelles, particularly nuclei, from a tissue. In some embodiments, this disclosure provides a method of processing a tissue sample involves performing enzymatic/chemical disruption of tissue in a chamber to produce disrupted tissue comprising released cells and/or nuclei and debris; separating the released cells and/or nuclei from the debris therein; and moving the released cells and/or nuclei. In some instances, the method comprises mechanical disruption of the tissue sample.
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公开(公告)号：US11441976B2
公开(公告)日：2022-09-13
申请号：US17513204
申请日：2021-10-28
申请人： S2 Genomics, Inc.
摘要： A system, methods, and apparatus are described to collect and prepare single cells, nuclei, subcellular components, and biomolecules from specimens including tissues. The system can perform enzymatic and/or physical disruption of the tissue to dissociate it into single-cells or nuclei in suspension or subcellular components including nucleic acids. In some embodiments, the titer of dissociated cells is monitored at intervals and the viability determined. In some embodiments, the processing is adjusted according to the measurements of the titer and viability. In some embodiments, the single-cells or nuclei in suspension are washed and resuspended in the buffer or media of choice. In some embodiments, the conditions are chosen to produce nuclei. In other embodiments, the single-cells or nuclei are purified by affinity paramagnetic bead processing. In some embodiments, matched bulk nucleic acid to the single-cells is produced. In other embodiments, single-cell libraries, or nuclei libraries, or matched bulk libraries, or bulk libraries are produced. The single cells or nuclei can then be further processed by FACS, DNA sequencing, mass spectrometry, fluorescence, or other methods. In other embodiments, the tissue processing is integrated with an analytical system to produce a sample-to-answer system such as a tissue-to-genomics system.
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公开(公告)号：US11719608B2
公开(公告)日：2023-08-08
申请号：US17581940
申请日：2022-01-23
申请人： S2 Genomics, Inc.
CPC分类号： G01N1/31 , C12M47/04 , C12M47/06 , C12N1/06 , C12N1/066 , C12N15/1003 , C12Q1/6851 , G01N1/286 , G01N2001/2866 , C12Q1/6851 , C12Q2525/161 , C12Q2525/179 , C12Q2563/179 , C12Q2565/514
摘要： This disclosure provides methods for producing a sample of subcellular organelles, particularly nuclei, from a tissue. In some embodiments, this disclosure provides a method of processing a tissue sample involves performing enzymatic/chemical disruption of tissue in a chamber to produce disrupted tissue comprising released cells and/or nuclei and debris; separating the released cells and/or nuclei from the debris therein; and moving the released cells and/or nuclei. In some instances, the method comprises mechanical disruption of the tissue sample.
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公开(公告)号：US20220146382A1
公开(公告)日：2022-05-12
申请号：US17581940
申请日：2022-01-23
申请人： S2 Genomics, Inc.
摘要： This disclosure provides methods for producing a sample of subcellular organelles, particularly nuclei, from a tissue. In some embodiments, this disclosure provides a method of processing a tissue sample involves performing enzymatic/chemical disruption of tissue in a chamber to produce disrupted tissue comprising released cells and/or nuclei and debris; separating the released cells and/or nuclei from the debris therein; and moving the released cells and/or nuclei. In some instances, the method comprises mechanical disruption of the tissue sample.
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公开(公告)号：US20220082479A1
公开(公告)日：2022-03-17
申请号：US17513204
申请日：2021-10-28
申请人： S2 Genomics, Inc.
摘要： A system, methods, and apparatus are described to collect and prepare single cells, nuclei, subcellular components, and biomolecules from specimens including tissues. The system can perform enzymatic and/or physical disruption of the tissue to dissociate it into single-cells or nuclei in suspension or subcellular components including nucleic acids. In some embodiments, the titer of dissociated cells is monitored at intervals and the viability determined. In some embodiments, the processing is adjusted according to the measurements of the titer and viability. In some embodiments, the single-cells or nuclei in suspension are washed and resuspended in the buffer or media of choice. In some embodiments, the conditions are chosen to produce nuclei. In other embodiments, the single-cells or nuclei are purified by affinity paramagnetic bead processing. In some embodiments, matched bulk nucleic acid to the single-cells is produced. In other embodiments, single-cell libraries, or nuclei libraries, or matched bulk libraries, or bulk libraries are produced. The single cells or nuclei can then be further processed by FACS, DNA sequencing, mass spectrometry, fluorescence, or other methods. In other embodiments, the tissue processing is integrated with an analytical system to produce a sample-to-answer system such as a tissue-to-genomics system.
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