公开(公告)号：US07655903B2

公开(公告)日：2010-02-02

申请号：US11737300

申请日：2007-04-19

申请人： Roman Zubarev ,  Alexander Misharin

发明人： Roman Zubarev ,  Alexander Misharin

IPC分类号： H01J49/36

CPC分类号： H01J49/38

摘要： An ion cyclotron resonance cell has at least one trapping electrode comprised of electrically isolated sections that are used for the detection of an induced ion image signal. Such an arrangement increases the sensitivity of image signal detection without a significant increase in the amplitude of parasitic harmonics. When a multielectrode detection arrangement is used, the resolving power of an analyzer incorporating such a cyclotron resonance cell multiplies without a corresponding sensitivity loss.

摘要翻译： 离子回旋共振池具有至少一个由用于检测诱导离子图像信号的电隔离部分组成的俘获电极。 这种布置增加了图像信号检测的灵敏度，而不会明显增加寄生谐波的幅度。 当使用多电极检测装置时，包含这种回旋共振元件的分析仪的分辨率倍增而没有相应的灵敏度损失。

公开(公告)号：US20060192100A1

公开(公告)日：2006-08-31

申请号：US11349551

申请日：2006-02-07

申请人： Roman Zubarev ,  Alexander Misharin ,  Oleg Silivra ,  Frank Kjeldsen

发明人： Roman Zubarev ,  Alexander Misharin ,  Oleg Silivra ,  Frank Kjeldsen

IPC分类号： H01J49/00 ,  B01D59/44

CPC分类号： H01J49/005 ,  G01N33/6848 ,  H01J49/02 ,  Y10S250/91 ,  Y10T436/17

摘要： The invention relates to a method and apparatus for the fragmentation of large molecules, especially biopolymers. The invention consists in reacting analyte ions with excited or radical neutral particles, whereby, at least in the case of bombardment of analyte ions with helium atoms from an FAB generator, a new type of fragmentation occurs which strongly resembles fragmentation by electron capture (ECD). The reactions may be performed in magnetic ion traps (ion cyclotron resonance cells, ICR), in RF ion traps according to Wolfgang Paul, in RF ion guides, or in free beams of analyte ions or neutral particles.

公开(公告)号：US08304715B2

公开(公告)日：2012-11-06

申请号：US12756015

申请日：2010-04-07

申请人： Alexander Misharin ,  Roman Zubarev

发明人： Alexander Misharin ,  Roman Zubarev

IPC分类号： H01J49/00 ,  B01D59/44

CPC分类号： H01J49/38

摘要： A measuring cell of an ICR mass spectrometer and a method of operating a measuring cell of the ICR mass spectrometer. The method and system trap ions in a first compartment of the ICR measuring cell by generating an electric potential well in the direction of the magnetic field with a minimum of the electric potential well located inside the first compartment. The method and system excite cyclotron motion of the ions trapped in the first compartment. The method and system transfer at least a part of the excited ions from the first compartment to a second compartment of the ICR measuring cell by displacement of a position of the minimum of the electric potential well from the first compartment to the second compartment. The ions are transferred by displacing the position of the minimum of the electric potential well from the first compartment to the second compartment preferably over a period of time equal to or longer than a characteristic period of ion oscillations along the direction of the magnetic field in the electric potential well. The method and system detect ion cyclotron motion of at least a part of the ions in the second compartment.

摘要翻译： ICR质谱仪的测量单元和操作ICR质谱仪的测量单元的方法。 该方法和系统通过在第一隔室内位于最小的电位下在磁场的方向上产生电位而在ICR测量池的第一隔室中捕获离子。 该方法和系统激发捕获在第一隔室中的离子的回旋加速器运动。 该方法和系统通过将最小电势井的位置从第一隔室移位到第二隔室，将激发的离子的至少一部分从第一隔室转移到ICR测量池的第二隔室。 通过将电位阱的最小值的位置从第一隔室移动到第二隔室，优选地在等于或长于沿着沿着磁场方向的离子振荡的特征周期的时间段 电势很好 该方法和系统检测第二隔室中至少一部分离子的离子回旋加速器运动。

公开(公告)号：US20110248159A1

公开(公告)日：2011-10-13

申请号：US12756015

申请日：2010-04-07

申请人： Alexander MISHARIN ,  Roman Zubarev

发明人： Alexander MISHARIN ,  Roman Zubarev

IPC分类号： H01J49/34 ,  H01J49/02

CPC分类号： H01J49/38

摘要： A measuring cell of an ICR mass spectrometer and a method of operating a measuring cell of the ICR mass spectrometer. The method and system trap ions in a first compartment of the ICR measuring cell by generating an electric potential well in the direction of the magnetic field with a minimum of the electric potential well located inside the first compartment. The method and system excite cyclotron motion of the ions trapped in the first compartment. The method and system transfer at least a part of the excited ions from the first compartment to a second compartment of the ICR measuring cell by displacement of a position of the minimum of the electric potential well from the first compartment to the second compartment. The ions are transferred by displacing the position of the minimum of the electric potential well from the first compartment to the second compartment preferably over a period of time equal to or longer than a characteristic period of ion oscillations along the direction of the magnetic field in the electric potential well. The method and system detect ion cyclotron motion of at least a part of the ions in the second compartment.

摘要翻译： ICR质谱仪的测量单元和操作ICR质谱仪的测量单元的方法。 该方法和系统通过在第一隔室内位于最小的电位下在磁场的方向上产生电位而在ICR测量池的第一隔室中捕获离子。 该方法和系统激发捕获在第一隔室中的离子的回旋加速器运动。 该方法和系统通过将最小电势井的位置从第一隔室移位到第二隔室，将激发的离子的至少一部分从第一隔室转移到ICR测量池的第二隔室。 通过将电位阱的最小值的位置从第一隔室移动到第二隔室，优选地在等于或长于沿着沿着磁场方向的离子振荡的特征周期的时间段 电势很好 该方法和系统检测第二隔室中至少一部分离子的离子回旋加速器运动。

公开(公告)号：US07476853B2

公开(公告)日：2009-01-13

申请号：US11349551

申请日：2006-02-07

申请人： Roman Zubarev ,  Alexander Misharin ,  Oleg Silivra ,  Frank Kjeldsen

发明人： Roman Zubarev ,  Alexander Misharin ,  Oleg Silivra ,  Frank Kjeldsen

IPC分类号： B01D59/44 ,  H01J49/00

CPC分类号： H01J49/005 ,  G01N33/6848 ,  H01J49/02 ,  Y10S250/91 ,  Y10T436/17

摘要： The invention relates to a method and apparatus for the fragmentation of large molecules, especially biopolymers. The invention consists in reacting analyte ions with excited or radical neutral particles, whereby, at least in the case of bombardment of analyte ions with helium atoms from an FAB generator, a new type of fragmentation occurs which strongly resembles fragmentation by electron capture (ECD). The reactions may be performed in magnetic ion traps (ion cyclotron resonance cells, ICR), in RF ion traps according to Wolfgang Paul, in RF ion guides, or in free beams of analyte ions or neutral particles.

摘要翻译： 本发明涉及一种用于大分子，特别是生物聚合物破碎的方法和装置。 本发明包括使分析物离子与激发或自由基中性颗粒反应，由此，至少在从FAB发生器轰击具有氦原子的分析物离子的情况下，发生类似于通过电子捕获（ECD）破碎的新型碎裂， 。 反应可以在磁离子阱（离子回旋共振池，ICR），RF离子阱中根据Wolfgang Paul，RF离子导向器或分析离子或中性粒子的自由光束进行。

公开(公告)号：US20070278402A1

公开(公告)日：2007-12-06

申请号：US11737300

申请日：2007-04-19

申请人： Roman Zubarev ,  Alexander Misharin

发明人： Roman Zubarev ,  Alexander Misharin

IPC分类号： B01D59/44

CPC分类号： H01J49/38

摘要： An ion cyclotron resonance cell has at least one trapping electrode comprised of electrically isolated sections that are used for the detection of an induced ion image signal. Such an arrangement increases the sensitivity of image signal detection without a significant increase in the amplitude of parasitic harmonics. When a multielectrode detection arrangement is used, the resolving power of an analyzer incorporating such a cyclotron resonance cell multiplies without a corresponding sensitivity loss.

摘要翻译： 离子回旋共振池具有至少一个由用于检测诱导离子图像信号的电隔离部分组成的俘获电极。 这种布置增加了图像信号检测的灵敏度，而不会明显增加寄生谐波的幅度。 当使用多电极检测装置时，包含这种回旋共振元件的分析仪的分辨率倍增而没有相应的灵敏度损失。

公开(公告)号：US20110121172A1

公开(公告)日：2011-05-26

申请号：US12992839

申请日：2009-05-04

申请人： Mikhail M. Savitski ,  Roman Zubarev

发明人： Mikhail M. Savitski ,  Roman Zubarev

IPC分类号： B01D59/44 ,  H01J49/26

CPC分类号： H01J49/0031 ,  G06F19/703 ,  H01J49/0045

摘要： A method of identifying precursor ion species from their fragments comprises obtaining mass spectra of a plurality of precursor ion species and their fragments to high mass accuracy. The fragment mass spectrum, obtained from fragmentation of multiple precursor ion species, is then scanned it identify pairs of fragments whose combined mass matches the mass of one of the precursor ion species. Once pairs of fragment ion shave been matched to precursor ions, the composite fragment ion spectrum is broken down into portions, one per fragment pair. Analysis continues until no further pairs are identified. A simplified fragment ion spectrum is then reconstructed for each precursor sample ion by stitching together the broken down sections of the composite fragment spectrum. The resultant reconstructed, simplified fragment spectra are sent to a search engine which returns a score-sorted list of likely candidates for each synthetic fragment ion spectrum.

摘要翻译： 从其片段鉴定前体离子种类的方法包括以高质量精度获得多种前体离子种类及其片段的质谱。 然后扫描从多个前体离子物质的断裂获得的片段质谱，鉴定其组合质量与一种前体离子物质的质量相匹配的片段对。 一对碎片离子与前体离子相匹配，复合片段离子谱被分解成部分，每个片段对一个。 分析继续进行，直到没有进一步的对被识别为止。 然后通过将复合片段谱的分解部分拼接在一起，为每个前体样品离子重建简化的片段离子谱。 所得到的重构的，简化的片段谱被发送到搜索引擎，该搜索引擎返回每个合成片段离子谱的可能候选的得分分类列表。

公开(公告)号：US20060255259A1

公开(公告)日：2006-11-16

申请号：US11403555

申请日：2006-04-13

申请人： Roman Zubarev ,  Mikhail Savitski ,  Michael Nielsen

发明人： Roman Zubarev ,  Mikhail Savitski ,  Michael Nielsen

IPC分类号： B01D59/44

CPC分类号： H01J49/0045

摘要： The invention relates to acquisition methods for fragment ion spectra of biopolymer molecules in tandem mass spectrometers which are coupled to separation devices. The invention provides a real-time method for calculating a quality coefficient for each fragment ion spectrum. The quality coefficient indicates whether the fragment ion spectrum can be used successfully for identifying the biopolymer molecule or whether it should be acquired once more, possibly with other acquisition parameters.

摘要翻译： 本发明涉及串联质谱仪中分离装置的生物聚合物分子的片段离子光谱的采集方法。 本发明提供了一种用于计算每个碎片离子光谱质量系数的实时方法。 质量系数表示碎片离子谱是否可以成功应用于识别生物聚合物分子，或者是否应该再次获得，可能还有其他采集参数。

公开(公告)号：US08481924B2

公开(公告)日：2013-07-09

申请号：US12992839

申请日：2009-05-04

申请人： Mikhail M. Savitski ,  Roman Zubarev

发明人： Mikhail M. Savitski ,  Roman Zubarev

IPC分类号： H01J49/26

CPC分类号： H01J49/0031 ,  G06F19/703 ,  H01J49/0045

摘要： A method of identifying precursor ion species from their fragments comprises obtaining mass spectra of a plurality of precursor ion species and their fragments to high mass accuracy. The fragment mass spectrum, obtained from fragmentation of multiple precursor ion species, is then scanned it identify pairs of fragments whose combined mass matches the mass of one of the precursor ion species. Once pairs of fragment ion shave been matched to precursor ions, the composite fragment ion spectrum is broken down into portions, one per fragment pair. Analysis continues until no further pairs are identified. A simplified fragment ion spectrum is then reconstructed for each precursor sample ion by stitching together the broken down sections of the composite fragment spectrum. The resultant reconstructed, simplified fragment spectra are sent to a search engine which returns a score-sorted list of likely candidates for each synthetic fragment ion spectrum.

摘要翻译： 从其片段鉴定前体离子种类的方法包括以高质量精度获得多种前体离子种类及其片段的质谱。 然后扫描从多个前体离子物质的断裂获得的片段质谱，鉴定其组合质量与一种前体离子物质的质量相匹配的片段对。 一对碎片离子与前体离子相匹配，复合片段离子谱被分解成部分，每个片段对一个。 分析继续进行，直到没有进一步的对被识别为止。 然后通过将复合片段谱的分解部分拼接在一起，为每个前体样品离子重建简化的片段离子谱。 所得到的重构的，简化的片段谱被发送到搜索引擎，该搜索引擎返回每个合成片段离子谱的可能候选的得分分类列表。

公开(公告)号：US08110793B2

公开(公告)日：2012-02-07

申请号：US11403555

申请日：2006-04-13

申请人： Roman Zubarev ,  Mikhail Savitski ,  Michael Lund Nielsen

发明人： Roman Zubarev ,  Mikhail Savitski ,  Michael Lund Nielsen

IPC分类号： B01D59/44

CPC分类号： H01J49/0045

摘要： The invention relates to acquisition methods for fragment ion spectra of biopolymer molecules in tandem mass spectrometers which are coupled to separation devices. The invention provides a real-time method for calculating a quality coefficient for each fragment ion spectrum. The quality coefficient indicates whether the fragment ion spectrum can be used successfully for identifying the biopolymer molecule or whether it should be acquired once more, possibly with other acquisition parameters.

摘要翻译： 本发明涉及串联质谱仪中分离装置的生物聚合物分子的片段离子光谱的采集方法。 本发明提供了一种用于计算每个碎片离子光谱质量系数的实时方法。 质量系数表示碎片离子谱是否可以成功应用于识别生物聚合物分子，或者是否应该再次获得，可能还有其他采集参数。