公开(公告)号：US06951971B1

公开(公告)日：2005-10-04

申请号：US09350393

申请日：1999-07-09

Applicant: Ray J. Wu ,  Tuan-Hua David Ho

Inventor： Ray J. Wu ,  Tuan-Hua David Ho

IPC: C12N15/29 ,  C12N15/82

CPC classification number: C12N15/8237 ,  C12N15/8273

Abstract: The present invention relates to a method for conferring tolerance to salt stress and drought stress in a monocot plant including transforming the monocot plant with an expression cassette comprising at least one ABRC unit, a minimal promoter, and a DNA molecule that increases tolerance to salt stress and drought stress in plants, wherein the at least one ABRC unit, the minimal promoter, and a DNA molecule are operably linked together to permit expression of the DNA molecule. The present invention also relates to a transgenic monocot plant transformed with a DNA molecule that increases tolerance to salt stress and drought stress operably linked to at least one ABRC unit and a minimal promoter.

Abstract translation: 本发明涉及一种赋予单子叶植物中盐胁迫和干旱胁迫耐受性的方法，包括用包含至少一个ABRC单元，最小启动子和增加耐盐胁迫的DNA分子的表达盒转化单子叶植物 和植物中的干旱胁迫，其中所述至少一个ABRC单元，所述最小启动子和DNA分子可操作地连接在一起以允许所述DNA分子的表达。 本发明还涉及用DNA分子转化的转基因单子叶植物，其增加了与至少一个ABRC单位和最小启动子连接的对盐胁迫和干旱胁迫的耐受性。

公开(公告)号：US4792602A

公开(公告)日：1988-12-20

申请号：US562045

申请日：1983-12-16

Applicant: Saran A. Narang ,  Ray J. Wu

Inventor： Saran A. Narang ,  Ray J. Wu

IPC: C07K14/62 ,  C12N15/66 ,  C12N15/00

CPC classification number: C12N15/66 ,  C07K14/62 ,  Y10S930/26

Abstract: A human-like proinsulin gene and its analogs, have been synthesized by a combination of chemical and enzymatic methods. A number of different human-like proinsulin gene analogs with altered C-chains have also been designed and can be readily constructed as described. As a part of the strategy, an adaptor for trimming DNA has been designed, synthesized and used to recover the A-chain insulin gene with the desired sequence from a hybrid plasmid; a related adaptor for trimming DNA has been designed to shorten the C-chain gene or any gene. The synthetic proinsulin gene has been joined to a replicable cloning vehicle and the hybrid DNA transferred to a host cell. The transformed host cell has been shown to contain the desired human-like proinsulin gene.

Abstract translation: 人类胰岛素原基因及其类似物已经通过化学和酶学方法的组合而合成。 已经设计了许多具有改变的C链的人类类胰岛素原基因类似物，并且可以如所述容易地构建。 作为策略的一部分，已经设计，合成用于修剪DNA的接头，并用于从杂交质粒中以期望的顺序恢复A链胰岛素基因; 已经设计了用于修剪DNA的相关接头，以缩短C链基因或任何基因。 合成胰岛素原基因已经连接到可复制的克隆载体上，并将杂交DNA转移到宿主细胞。 已经显示转化的宿主细胞含有所需的人样胰岛素原基因。

公开(公告)号：US07271004B2

公开(公告)日：2007-09-18

申请号：US10834786

申请日：2004-04-29

Applicant: Ray J. Wu ,  Ajay K. Garg ,  Ju-Kon Kim

Inventor： Ray J. Wu ,  Ajay K. Garg ,  Ju-Kon Kim

IPC: A01H5/00 ,  A01H5/10 ,  C12N15/82 ,  C12N5/04

CPC classification number: C12N15/8261 ,  Y02A40/146

Abstract: An isolated nucleic acid construct including a nucleic acid molecule encoding a light-labile, phytochrome A, a light-inducible promoter which is 5′ to the nucleic acid molecule encoding a light-labile, phytochrome A, and a terminator region which is 3′ to the nucleic acid molecule encoding a light-labile, phytochrome A is disclosed. Methods for regulating a plant's canopy architecture and regulating a plant's seed yield, which involve transgenic plants or transgenic plant seeds including an isolated nucleic acid construct according to the present invention, are also disclosed.

Abstract translation: 分离的核酸构建体，其包含编码光不稳定的植物色素A的核酸分子，与编码光不稳定的植物色素A的核酸分子成为5'的光诱导型启动子，和3' 涉及编码光不稳定的植物色素A的核酸分子。 还公开了用于调节植物冠层结构和调节植物种子产量的方法，其涉及包括根据本发明的分离的核酸构建体的转基因植物或转基因植物种子。

公开(公告)号：US4321365A

公开(公告)日：1982-03-23

申请号：US843422

申请日：1977-10-19

Applicant: Ray J. Wu ,  Chander P. Bahl ,  Saran A. Narang

Inventor： Ray J. Wu ,  Chander P. Bahl ,  Saran A. Narang

IPC: C07H21/00 ,  C12N15/00 ,  C07H21/04

CPC classification number: C07H21/00 ,  C12N15/00

Abstract: Synthetic oligonucleotides have been designed and prepared which are useful in the molecular cloning of a variety of DNA molecules. By means of such oligonucleotides, genetic informational material, e.g., DNA, can be joined to a cloning vehicle and transferred into host cells by transformation. Additionally, a method for determining whether genetic informational material has been transferred into transformed host cells has been developed.

Abstract translation: 已经设计和制备了合成寡核苷酸，其可用于多种DNA分子的分子克隆。 通过这样的寡核苷酸，遗传信息材料（例如DNA）可以连接到克隆载体上并通过转化转移到宿主细胞中。 另外，已经开发了用于确定遗传信息材料是否已转移到转化的宿主细胞中的方法。

公开(公告)号：US5981842A

公开(公告)日：1999-11-09

申请号：US730659

申请日：1996-10-11

Applicant: Ray J. Wu ,  Tuan-Hua D. Ho

Inventor： Ray J. Wu ,  Tuan-Hua D. Ho

IPC: C07K14/415 ,  C12N15/29 ,  C12N15/82 ,  A01H4/00 ,  A01H5/00

CPC classification number: C07K14/415 ,  C12N15/8273

Abstract: The present invention is directed to a method of producing a cereal plant cell or protoplast useful for regeneration of a water stress or salt stress tolerant cereal plant by transforming the cereal plant cell or protoplast with a nucleic acid encoding a late embryogenesis abundant protein. A transgenic cereal plant or cereal plant cell or protoplast transformed with a nucleic acid encoding a late embryogenesis abundant protein is also provided. An LEA protein gene, HVA1, from barley (Hordeum vulgare L.) was transformed into rice (Oryza sativa L.) plants. The resulting transgenic rice plants accumulate the HVA1 protein in both leaves and roots. Transgenic rice plants showed significantly increased tolerance to water stress (drought) and salt stress.

Abstract translation: 本发明涉及通过用编码晚期胚胎发生丰富蛋白质的核酸转化谷物植物细胞或原生质体来生产可用于再生水分胁迫或耐盐胁迫谷物植物的谷物植物细胞或原生质体的方法。 还提供了用编码晚期胚胎发生丰富蛋白质的核酸转化的转基因谷物植物或谷物植物细胞或原生质体。 将来自大麦（Hordeum vulgare L.）的LEA蛋白基因HVA1转化到水稻（Oryza sativa L.）植物中。 所得转基因水稻在叶和根中积累HVA1蛋白。 转基因水稻对水胁迫（干旱）和盐胁迫的耐受性显着增加。

公开(公告)号：US08889949B2

公开(公告)日：2014-11-18

申请号：US11619335

申请日：2007-01-03

Applicant: Ray J. Wu ,  Ajay K. Garg ,  Ju-Kon Kim ,  Baek-Hie Nahm ,  Yang-Do Choi ,  In-Cheol Jang ,  Won-Bin Choi ,  Yeon-Seak Kim ,  Chung-Ho Kim ,  Sang-Ik Song

Inventor： Ray J. Wu ,  Ajay K. Garg ,  Ju-Kon Kim ,  Baek-Hie Nahm ,  Yang-Do Choi ,  In-Cheol Jang ,  Won-Bin Choi ,  Yeon-Seak Kim ,  Chung-Ho Kim ,  Sang-Ik Song

IPC: A01H1/00 ,  A01H5/10 ,  C12N5/04 ,  C12N15/82 ,  C12N15/87 ,  C12P21/04 ,  C12N9/16 ,  C12N9/10

CPC classification number: C12Y204/01015 ,  C07K2319/00 ,  C12N9/1051 ,  C12N9/16 ,  C12N15/8273 ,  C12Y301/03012

Abstract: The present invention relates to a method for increasing resistance of monocot plants against abiotic stress which comprises a step of transforming monocot plants with a recombinant plasmid containing a fused gene (TPSP) of trehalose-6-phosphate synthetase (TPS) gene and trehalose-6-phosphate phosphatase (TPP) gene to express the TPSP gene while maintaining normal growth and development characteristics. The present invention can increase the resistance of monocot plants against various stresses so that it can greatly contribute to the improvement of production and quality of valuable agricultural crops. The present invention also relates to a transgenic monocot plant, plant cell, or protoplast transformed with a nucleic acid encoding an enzyme for trehalose biosynthesis, under control of an inducible promoter, that increases tolerance to low temperature, salt, and water stress.

Abstract translation: 本发明涉及一种增加单子叶植物抗非生物胁迫抗性的方法，该方法包括用含有海藻糖-6-磷酸合成酶（TPS）基因和海藻糖-6（TPS）基因的融合基因（TPSP）的重组质粒转化单子叶植物的步骤 磷酸磷酸酶（TPP）基因表达TPSP基因，同时保持正常的生长发育特征。 本发明可以增加单子叶植物对各种胁迫的抗性，从而可以大大有助于提高有价值的农作物的生产和质量。 本发明还涉及在诱导型启动子控制下用编码海藻糖生物合成酶的核酸转化的转基因单子叶植物，植物细胞或原生质体，其增加对低温，盐和水胁迫的耐受性。

公开(公告)号：US4617384A

公开(公告)日：1986-10-14

申请号：US426457

申请日：1982-09-29

Applicant: Saran A. Narang ,  Ray J. Wu

Inventor： Saran A. Narang ,  Ray J. Wu

IPC: C07K14/62 ,  C12N15/10 ,  C12N15/66 ,  C12N15/70 ,  C07H21/04 ,  C12N15/00

CPC classification number: C12N15/10 ,  C07K14/62 ,  C12N15/66 ,  C12N15/70 ,  Y10S930/26

Abstract: Adaptor molecules have been prepared to comprise either start or stop signals for protein synthesis, in addition to recognition sites for restriction endonucleases. Separate adaptors may be used in a symmetrical duplex form. The start adaptor may include nucleotide base inserts to provide the correct reading frame of the triplet code in a DNA sequence with inappropriate reading frame. Insulin A-chain and B-chain genes of the human type, have been synthesized with the appropriate adaptor molecules provided on each end. The adapted DNA genes have been joined to replicable cloning vehicles and the hybrid DNA transferred to a host cell. The transformed host cell has been shown to contain the desired insulin gene.

Abstract translation: 除了限制性内切核酸酶的识别位点之外，已经准备了适配子分子以包含用于蛋白质合成的起始或停止信号。 单独的适配器可以以对称双工形式使用。 启动适配器可以包括核苷酸碱基插入物，以在不适当阅读框架的DNA序列中提供三重码的正确阅读框。 人类的胰岛素A链和B链基因已经与每端提供的适当的衔接子分子合成。 已经将适应的DNA基因连接到可复制的克隆载体上，并将杂交DNA转移到宿主细胞。 已经显示转化的宿主细胞含有所需的胰岛素基因。