公开(公告)号：US07476651B2

公开(公告)日：2009-01-13

申请号：US11903030

申请日：2007-09-20

Applicant: Paul Schimmel ,  Keisuke Wakasugi ,  Martin Friedlander

Inventor： Paul Schimmel ,  Keisuke Wakasugi ,  Martin Friedlander

IPC: A61K38/00 ,  C12N9/10

CPC classification number: C12N9/93 ,  A61K38/00 ,  C07H21/04 ,  C07K14/515 ,  C07K14/521 ,  C12Y601/01002 ,  Y10S435/975

Abstract: The invention provides a method for inhibiting ocular neovascularization in a patient. The method comprises administering to a patient an ocular neovascularization inhibiting amount of a water-soluble polypeptide selected from the group consisting of SEQ ID NO: 12, SEQ ID NO: 7, and an ocular neovascularization inhibiting fragment thereof, which includes at least one of amino acid residue signature sequences HVGH (SEQ ID NO:10) and KMSAS (SEQ ID NO:11). A method for assaying the angiogenesis inhibiting activity of a composition is also provided.

Abstract translation: 本发明提供一种抑制患者眼新生血管形成的方法。 该方法包括向患者施用眼新生血管形成抑制量的选自SEQ ID NO：12，SEQ ID NO：7的水溶性多肽和其新生血管形成抑制片段，其包括至少一种 氨基酸残基特征序列HVGH（SEQ ID NO：10）和KMSAS（SEQ ID NO：11）。 还提供了用于测定组合物的血管发生抑制活性的方法。

公开(公告)号：US20120238620A1

公开(公告)日：2012-09-20

申请号：US13229866

申请日：2011-09-12

Applicant: Paul Schimmel ,  Keisuke Wakasugi ,  Martin Friedlander

Inventor： Paul Schimmel ,  Keisuke Wakasugi ,  Martin Friedlander

IPC: A61K31/713 ,  C12N15/861 ,  C12N15/869 ,  C12N15/863 ,  A61P9/00 ,  C12N5/10 ,  C12N1/19 ,  C12N1/15 ,  C12N1/21 ,  C12N15/52 ,  C12N15/85 ,  C12N15/867

CPC classification number: C12N9/93 ,  A61K38/00 ,  C07H21/04 ,  C07K14/515 ,  C07K14/521 ,  C12Y601/01002 ,  Y10S435/975

Abstract: The invention provides an isolated nucleic acid encoding a polypeptide capable of inhibiting angiogenesis or neovascularization, wherein the nucleic acid comprises a first polynucleotide sequence comprising a coding sequence at least 95 percent identical to a sequence selected from the group consisting of a polynucleotide SEQ ID NO:6, a polynucleotide that encodes a polypeptide of SEQ ID NO:12, and a polynucleotide that encodes a fragment of the polypeptide of SEQ ID NO:12; and wherein the nucleic acid does not encode for the amino acid sequence of amino acids 71-93 of SEQ ID NO:1. Pharmaceutical compositions, vectors, and methods for inhibiting neovascularization or angiogenesis comprising or utilizing the nucleic acids also are provided.

Abstract translation: 本发明提供了编码能够抑制血管生成或新生血管形成的多肽的分离的核酸，其中所述核酸包含第一多核苷酸序列，所述第一多核苷酸序列包含与选自多核苷酸SEQ ID NO： 6，编码SEQ ID NO：12的多肽的多核苷酸和编码SEQ ID NO：12的多肽的片段的多核苷酸; 并且其中所述核酸不编码SEQ ID NO：1的氨基酸71-93的氨基酸序列。 还提供了用于抑制新生血管形成或血管发生的药物组合物，载体和方法，其包含或利用核酸。

公开(公告)号：US07413885B2

公开(公告)日：2008-08-19

申请号：US11803326

申请日：2007-05-14

Applicant: Paul Schimmel ,  Keisuke Wakasugi ,  Martin Friedlander

Inventor： Paul Schimmel ,  Keisuke Wakasugi ,  Martin Friedlander

IPC: C12N9/10 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04

CPC classification number: C12N9/93 ,  A61K38/00 ,  C07H21/04 ,  C07K14/515 ,  C07K14/521 ,  C12Y601/01002 ,  Y10S435/975

Abstract: The invention provides an isolated nucleic acid encoding a water-soluble polypeptide fragment of human tryptophanyl-tRNA synthetase, which is useful for the inhibition of angiogenesis. The nucleic acid comprises a polynucleotide of SEQ ID NO: 6, a polynucleotide hybridizable to SEQ ID NO: 6, a polynucleotide that encodes the polypeptide of SEQ ID NO: 7, a polynucleotide that encodes a polypeptide of SEQ ID NO: 12, a polynucleotide that encodes a polypeptide epitope of SEQ ID NO: 7, or a polynucleotide that is hybridizable to a polynucleotide that encodes a polypeptide epitope of SEQ ID NO: 7. Vectors and recombinant cells comprising the nucleic acid are also provided.

Abstract translation: 本发明提供编码人色氨酰-tRNA合成酶的水溶性多肽片段的分离的核酸，其可用于抑制血管发生。 核酸包含SEQ ID NO：6的多核苷酸，可与SEQ ID NO：6杂交的多核苷酸，编码SEQ ID NO：7的多肽的多核苷酸，编码SEQ ID NO：12的多肽的多核苷酸， 编码SEQ ID NO：7的多肽表位的多核苷酸或可与编码SEQ ID NO：7的多肽表位的多核苷酸杂交的多核苷酸。还提供了载体和包含该核酸的重组细胞。

公开(公告)号：US20080064639A1

公开(公告)日：2008-03-13

申请号：US11903030

申请日：2007-09-20

Applicant: Paul Schimmel ,  Keisuke Wakasugi ,  Martin Friedlander

Inventor： Paul Schimmel ,  Keisuke Wakasugi ,  Martin Friedlander

IPC: A61K38/00 ,  G01N33/53

CPC classification number: C12N9/93 ,  A61K38/00 ,  C07H21/04 ,  C07K14/515 ,  C07K14/521 ,  C12Y601/01002 ,  Y10S435/975

Abstract: The invention provides a method for inhibiting ocular neovascularization in a patient. The method comprises administering to a patient an ocular neovascularization inhibiting amount of a water-soluble polypeptide selected from the group consisting of SEQ ID NO: 12, SEQ ID NO: 7, and an ocular neovascularization inhibiting fragment thereof, which includes at least one of amino acid residue signature sequences HVGH (SEQ ID NO:10) and KMSAS (SEQ ID NO:11). A method for assaying the angiogenesis inhibiting activity of a composition is also provided

Abstract translation: 本发明提供一种抑制患者眼新生血管形成的方法。 该方法包括向患者施用眼新生血管形成抑制量的选自SEQ ID NO：12，SEQ ID NO：7的水溶性多肽和其新生血管形成抑制片段，其包括至少一种 氨基酸残基特征序列HVGH（SEQ ID NO：10）和KMSAS（SEQ ID NO：11）。 还提供了用于测定组合物的血管发生抑制活性的方法

公开(公告)号：US20070218527A1

公开(公告)日：2007-09-20

申请号：US11803326

申请日：2007-05-14

Applicant: Paul Schimmel ,  Keisuke Wakasugi ,  Martin Friedlander

Inventor： Paul Schimmel ,  Keisuke Wakasugi ,  Martin Friedlander

IPC: C12P21/06 ,  C07H21/02 ,  C12N15/63 ,  C12N15/64 ,  C12N5/10 ,  C12P13/22

CPC classification number: C12N9/93 ,  A61K38/00 ,  C07H21/04 ,  C07K14/515 ,  C07K14/521 ,  C12Y601/01002 ,  Y10S435/975

Abstract: The invention provides an isolated nucleic acid encoding a water-soluble polypeptide fragment of human tryptophanyl-tRNA synthetase, which is useful for the inhibition of angiogenesis. The nucleic acid comprises a polynucleotide of SEQ ID NO: 6, a polynucleotide hybridizable to SEQ ID NO: 6, a polynucleotide that encodes the polypeptide of SEQ ID NO: 7, a polynucleotide that encodes a polypeptide of SEQ ID NO: 12, a polynucleotide that encodes a polypeptide epitope of SEQ ID NO: 7, or a polynucleotide that is hybridizable to a polynucleotide that encodes a polypeptide epitope of SEQ ID NO: 7. Vectors and recombinant cells comprising the nucleic acid are also provided.

Abstract translation: 本发明提供编码人色氨酰-tRNA合成酶的水溶性多肽片段的分离的核酸，其可用于抑制血管发生。 核酸包含SEQ ID NO：6的多核苷酸，可与SEQ ID NO：6杂交的多核苷酸，编码SEQ ID NO：7的多肽的多核苷酸，编码SEQ ID NO：12的多肽的多核苷酸， 编码SEQ ID NO：7的多肽表位的多核苷酸或可与编码SEQ ID NO：7的多肽表位的多核苷酸杂交的多核苷酸。还提供了载体和包含该核酸的重组细胞。

公开(公告)号：US08796237B2

公开(公告)日：2014-08-05

申请号：US13229866

申请日：2011-09-12

Applicant: Paul Schimmel ,  Keisuke Wakasugi ,  Martin Friedlander

Inventor： Paul Schimmel ,  Keisuke Wakasugi ,  Martin Friedlander

IPC: C07H21/04 ,  C07K14/515

CPC classification number: C12N9/93 ,  A61K38/00 ,  C07H21/04 ,  C07K14/515 ,  C07K14/521 ,  C12Y601/01002 ,  Y10S435/975

Abstract: The invention provides an isolated nucleic acid encoding a polypeptide capable of inhibiting angiogenesis or neovascularization, wherein the nucleic acid comprises a first polynucleotide sequence comprising a coding sequence at least 95 percent identical to a sequence selected from the group consisting of a polynucleotide SEQ ID NO:6, a polynucleotide that encodes a polypeptide of SEQ ID NO:12, and a polynucleotide that encodes a fragment of the polypeptide of SEQ ID NO:12; and wherein the nucleic acid does not encode for the amino acid sequence of amino acids 71-93 of SEQ ID NO:1. Pharmaceutical compositions, vectors, and methods for inhibiting neovascularization or angiogenesis comprising or utilizing the nucleic acids also are provided.

Abstract translation: 本发明提供了编码能够抑制血管生成或新生血管形成的多肽的分离的核酸，其中所述核酸包含第一多核苷酸序列，所述第一多核苷酸序列包含与选自多核苷酸SEQ ID NO： 6，编码SEQ ID NO：12的多肽的多核苷酸和编码SEQ ID NO：12的多肽的片段的多核苷酸; 并且其中所述核酸不编码SEQ ID NO：1的氨基酸71-93的氨基酸序列。 还提供了用于抑制新生血管形成或血管发生的药物组合物，载体和方法，其包含或利用核酸。

公开(公告)号：US08017593B2

公开(公告)日：2011-09-13

申请号：US12319822

申请日：2009-01-13

Applicant: Paul Schimmel ,  Keisuke Wakasugi ,  Martin Friedlander

Inventor： Paul Schimmel ,  Keisuke Wakasugi ,  Martin Friedlander

IPC: A61K48/00

CPC classification number: C12N9/93 ,  A61K38/00 ,  C07H21/04 ,  C07K14/515 ,  C07K14/521 ,  C12Y601/01002 ,  Y10S435/975

Abstract: The invention provides a method for inhibiting ocular neovascularization in a patient. The method comprises administering to a patient an ocular neovascularization inhibiting amount of a water-soluble polypeptide selected from the group consisting of SEQ ID NO: 12, SEQ ID NO: 7, and an ocular neovascularization inhibiting fragment thereof, which includes at least one of amino acid residue signature sequences HVGH (SEQ ID NO:10) and KMSAS (SEQ ID NO:11). A method for assaying the angiogenesis inhibiting activity of a composition is also provided.

Abstract translation: 本发明提供一种抑制患者眼新生血管形成的方法。 该方法包括向患者施用眼新生血管形成抑制量的选自SEQ ID NO：12，SEQ ID NO：7的水溶性多肽和其新生血管形成抑制片段，其包括至少一种 氨基酸残基特征序列HVGH（SEQ ID NO：10）和KMSAS（SEQ ID NO：11）。 还提供了用于测定组合物的血管发生抑制活性的方法。

公开(公告)号：US20090275643A1

公开(公告)日：2009-11-05

申请号：US12319822

申请日：2009-01-13

Applicant: Paul Schimmel ,  Keisuke Wakasugi ,  Martin Friedlander

Inventor： Paul Schimmel ,  Keisuke Wakasugi ,  Martin Friedlander

IPC: A61K31/711 ,  G01N33/483

CPC classification number: C12N9/93 ,  A61K38/00 ,  C07H21/04 ,  C07K14/515 ,  C07K14/521 ,  C12Y601/01002 ,  Y10S435/975

Abstract: The invention provides a method for inhibiting ocular neovascularization in a patient. The method comprises administering to a patient an ocular neovascularization inhibiting amount of a water-soluble polypeptide selected from the group consisting of SEQ ID NO: 12, SEQ ID NO: 7, and an ocular neovascularization inhibiting fragment thereof, which includes at least one of amino acid residue signature sequences HVGH (SEQ ID NO:10) and KMSAS (SEQ ID NO:11). A method for assaying the angiogenesis inhibiting activity of a composition is also provided

Abstract translation: 本发明提供一种抑制患者眼新生血管形成的方法。 该方法包括向患者施用眼新生血管形成抑制量的选自SEQ ID NO：12，SEQ ID NO：7的水溶性多肽和其新生血管形成抑制片段，其包括至少一种 氨基酸残基特征序列HVGH（SEQ ID NO：10）和KMSAS（SEQ ID NO：11）。 还提供了用于测定组合物的血管发生抑制活性的方法

公开(公告)号：US07273844B2

公开(公告)日：2007-09-25

申请号：US10982014

申请日：2004-11-04

Applicant: Paul Schimmel ,  Keisuke Wakasugi ,  Martin Friedlander

Inventor： Paul Schimmel ,  Keisuke Wakasugi ,  Martin Friedlander

IPC: A61K38/00 ,  A61K38/45 ,  G01N33/53

CPC classification number: C12N9/93 ,  A61K38/00 ,  C07H21/04 ,  C07K14/515 ,  C07K14/521 ,  C12Y601/01002 ,  Y10S435/975

Abstract: An isolated, water-soluble polypeptide fragment of human tryptophanyl-tRNA synthetase is useful for the inhibition of angiogenesis. The polypeptide has the amino acid residue sequence shown in SEQ ID NO: 7, SEQ ID NO: 12, or an angiogenesis inhibiting fragment thereof, the fragment including at least one of amino acid residue signature sequences HVGH (SEQ ID NO:10) and KMSAS (SEQ ID NO:11). Methods of using the polypeptide to inhibit angiogenesis are also provided.

Abstract translation: 人色氨酰-tRNA合成酶的分离的水溶性多肽片段可用于抑制血管发生。 该多肽具有SEQ ID NO：7，SEQ ID NO：12所示的氨基酸残基或其血管生成抑制片段，该片段包括氨基酸残基特征序列HVGH（SEQ ID NO：10）和 KMSAS（SEQ ID NO：11）。 还提供了使用该多肽抑制血管生成的方法。

公开(公告)号：US20050197298A1

公开(公告)日：2005-09-08

申请号：US10982014

申请日：2004-11-04

Applicant: Paul Schimmel ,  Keisuke Wakasugi ,  Martin Friedlander

Inventor： Paul Schimmel ,  Keisuke Wakasugi ,  Martin Friedlander

IPC: A61K38/00 ,  C07H21/04 ,  C07K14/515 ,  C07K14/52 ,  C12N9/00 ,  A61K38/08 ,  A61K48/00 ,  C12N9/22 ,  C12Q1/68

CPC classification number: C12N9/93 ,  A61K38/00 ,  C07H21/04 ,  C07K14/515 ,  C07K14/521 ,  C12Y601/01002 ,  Y10S435/975

Abstract: An isolated, water-soluble polypeptide fragment of human tryptophanyl-tRNA synthetase is useful for the inhibition of angiogenesis. The polypeptide has the amino acid residue sequence shown in SEQ ID NO: 7, SEQ ID NO: 12, or an angiogenesis inhibiting fragment thereof, the fragment including at least one of amino acid residue signature sequences HVGH (SEQ ID NO:10) and KMSAS (SEQ ID NO:11). Methods of using the polypeptide to inhibit angiogenesis are also provided.

Abstract translation: 人色氨酰-tRNA合成酶的分离的水溶性多肽片段可用于抑制血管发生。 该多肽具有SEQ ID NO：7，SEQ ID NO：12所示的氨基酸残基或其血管生成抑制片段，该片段包括氨基酸残基特征序列HVGH（SEQ ID NO：10）和 KMSAS（SEQ ID NO：11）。 还提供了使用该多肽抑制血管生成的方法。