公开(公告)号：US07476651B2

公开(公告)日：2009-01-13

申请号：US11903030

申请日：2007-09-20

Applicant: Paul Schimmel ,  Keisuke Wakasugi ,  Martin Friedlander

Inventor： Paul Schimmel ,  Keisuke Wakasugi ,  Martin Friedlander

IPC: A61K38/00 ,  C12N9/10

CPC classification number: C12N9/93 ,  A61K38/00 ,  C07H21/04 ,  C07K14/515 ,  C07K14/521 ,  C12Y601/01002 ,  Y10S435/975

Abstract: The invention provides a method for inhibiting ocular neovascularization in a patient. The method comprises administering to a patient an ocular neovascularization inhibiting amount of a water-soluble polypeptide selected from the group consisting of SEQ ID NO: 12, SEQ ID NO: 7, and an ocular neovascularization inhibiting fragment thereof, which includes at least one of amino acid residue signature sequences HVGH (SEQ ID NO:10) and KMSAS (SEQ ID NO:11). A method for assaying the angiogenesis inhibiting activity of a composition is also provided.

Abstract translation: 本发明提供一种抑制患者眼新生血管形成的方法。 该方法包括向患者施用眼新生血管形成抑制量的选自SEQ ID NO：12，SEQ ID NO：7的水溶性多肽和其新生血管形成抑制片段，其包括至少一种 氨基酸残基特征序列HVGH（SEQ ID NO：10）和KMSAS（SEQ ID NO：11）。 还提供了用于测定组合物的血管发生抑制活性的方法。

公开(公告)号：US20060216745A1

公开(公告)日：2006-09-28

申请号：US11444924

申请日：2006-06-01

Applicant: Paul Schimmel ,  Keisuke Wakasugi

Inventor： Paul Schimmel ,  Keisuke Wakasugi

IPC: C12Q1/68 ,  C07H21/04 ,  C12P21/06 ,  C12N9/22

CPC classification number: C12N9/93 ,  A61K38/00 ,  C07H21/04 ,  C07K14/515 ,  C07K14/521

Abstract: Compositions comprising truncated tryptophanyl-tRNA synthetase polypeptides useful for regulating angiogenesis, as well as nucleic acids encoding such tRNA synthetase polypeptides are described. Methods of making and using such compositions are also disclosed.

公开(公告)号：US07067126B2

公开(公告)日：2006-06-27

申请号：US10240532

申请日：2001-03-21

Applicant: Paul Schimmel ,  Keisuke Wakasugi

Inventor： Paul Schimmel ,  Keisuke Wakasugi

IPC: A61K38/51 ,  C12N9/88 ,  C12N15/79 ,  C07H21/04

CPC classification number: C12N9/93 ,  A61K38/00 ,  C07H21/04 ,  C07K14/515 ,  C07K14/521

Abstract: Compositions comprising truncated tryptophanyl-tRNA synthetase polypeptides useful for regulating angiogenesis, as well as nucleic acids encoding such tRNA synthetase polypeptides are described. Methods of making and using such compositions are also disclosed.

公开(公告)号：US08629253B2

公开(公告)日：2014-01-14

申请号：US11590434

申请日：2006-10-31

Applicant: Paul Schimmel ,  Keisuke Wakasugi

Inventor： Paul Schimmel ,  Keisuke Wakasugi

IPC: C07H21/04

CPC classification number: A61K38/53 ,  A61K38/00 ,  C07H21/04 ,  C12N9/93

Abstract: Nucleic acids encoding tRNA synthetase polypeptides useful for regulating angiogenesis are disclosed. Methods of making and using such nucleic acids are also disclosed.

Abstract translation: 公开了编码用于调节血管生成的tRNA合成酶多肽的核酸。 还公开了制备和使用这些核酸的方法。

公开(公告)号：US20120263702A1

公开(公告)日：2012-10-18

申请号：US13434933

申请日：2012-03-30

Applicant: Paul SCHIMMEL ,  Keisuke WAKASUGI

Inventor： Paul SCHIMMEL ,  Keisuke WAKASUGI

IPC: A61K38/53 ,  A61K31/711 ,  A61P35/00 ,  C12N9/00

CPC classification number: C12N9/93 ,  A61K38/00 ,  C07H21/04 ,  C07K14/515 ,  C07K14/521

Abstract: Pharmaceutical compositions comprising truncated tryptophanyl-tRNA synthetase polypeptides useful for regulating angiogenesis an nucleic acids encoding such tRNA synthetase polypeptides are described. Methods of making and using such compositions are also disclosed.

Abstract translation: 描述了包含用于调节血管生成的截短的色氨酰-tRNA合成酶多肽的药物组合物，其编码这种tRNA合成酶多肽的核酸。 还公开了制备和使用这些组合物的方法。

公开(公告)号：US08101566B2

公开(公告)日：2012-01-24

申请号：US12655717

申请日：2010-01-06

Applicant: Paul Schimmel ,  Keisuke Wakasugi

Inventor： Paul Schimmel ,  Keisuke Wakasugi

IPC: A61K38/00

CPC classification number: C12N9/93 ,  A61K38/00

Abstract: A method of suppressing angiogenesis in a mammal (e.g., for treating tumors, tumor metastasis or a condition that would benefit from decreased angiogenesis) comprises the step of administering to the mammal an angiostatically effective amount of a pharmaceutical composition comprising an isolated antiangiogenic truncated tryptophanyl-tRNA synthetase (TrpRS) polypeptide or an isolated nucleic acid that comprises a polynucleotide sequence that encodes the truncated TrpRS polypeptide. The truncated TrpRS polypeptide comprises residues 71-471 of SEQ ID NO: 10, residues 48-471 of SEQ ID NO: 10, or a polypeptide of approximately 47 kD molecular weight produced by cleavage of the polypeptide of SEQ ID NO: 10 with polymorphonuclear leucocyte elastase.

Abstract translation: 抑制哺乳动物血管发生的方法（例如，用于治疗肿瘤，肿瘤转移或有益于血管生成减少的病症）包括向哺乳动物施用血管生成有效量的药物组合物的步骤，其包含分离的抗血管生成截短的色氨酸 - tRNA合成酶（TrpRS）多肽或分离的核酸，其包含编码截短的TrpRS多肽的多核苷酸序列。 截短的TrpRS多肽包含SEQ ID NO：10的残基71-471，SEQ ID NO：10的残基48-471或通过用多核型切割SEQ ID NO：10的多肽产生的约47kD分子量的多肽 白细胞弹性蛋白酶。

公开(公告)号：US07901917B2

公开(公告)日：2011-03-08

申请号：US12386428

申请日：2009-04-17

Applicant: Paul Schimmel ,  Keisuke Wakasugi

Inventor： Paul Schimmel ,  Keisuke Wakasugi

IPC: C12N9/14 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04

CPC classification number: C12N9/93 ,  A61K38/00 ,  C07H21/04 ,  C07K14/515 ,  C07K14/521

Abstract: Compositions comprising truncated tryptophanyl-tRNA synthetase polypeptides useful for regulating angiogenesis, as well as nucleic acids encoding such tRNA synthetase polypeptides are described. Methods of making and using such compositions are also disclosed.

Abstract translation: 描述了包含用于调节血管发生的截短的色氨酰-tRNA合成酶多肽的组合物，以及编码这种tRNA合成酶多肽的核酸。 还公开了制备和使用这些组合物的方法。

公开(公告)号：US20090227662A1

公开(公告)日：2009-09-10

申请号：US12386428

申请日：2009-04-17

Applicant: Paul Schimmel ,  Keisuke Wakasugi

Inventor： Paul Schimmel ,  Keisuke Wakasugi

IPC: A61K31/7088 ,  C07H21/00 ,  C12N15/74 ,  C12N1/00 ,  C12P21/00 ,  A61P35/00

CPC classification number: C12N9/93 ,  A61K38/00 ,  C07H21/04 ,  C07K14/515 ,  C07K14/521

Abstract: Compositions comprising truncated tryptophanyl-tRNA synthetase polypeptides useful for regulating angiogenesis, as well as nucleic acids encoding such tRNA synthetase polypeptides are described. Methods of making and using such compositions are also disclosed.

Abstract translation: 描述了包含用于调节血管生成的截短的色氨酰-tRNA合成酶多肽的组合物，以及编码这种tRNA合成酶多肽的核酸。 还公开了制备和使用这些组合物的方法。

公开(公告)号：US20070218527A1

公开(公告)日：2007-09-20

申请号：US11803326

申请日：2007-05-14

Applicant: Paul Schimmel ,  Keisuke Wakasugi ,  Martin Friedlander

Inventor： Paul Schimmel ,  Keisuke Wakasugi ,  Martin Friedlander

IPC: C12P21/06 ,  C07H21/02 ,  C12N15/63 ,  C12N15/64 ,  C12N5/10 ,  C12P13/22

CPC classification number: C12N9/93 ,  A61K38/00 ,  C07H21/04 ,  C07K14/515 ,  C07K14/521 ,  C12Y601/01002 ,  Y10S435/975

Abstract: The invention provides an isolated nucleic acid encoding a water-soluble polypeptide fragment of human tryptophanyl-tRNA synthetase, which is useful for the inhibition of angiogenesis. The nucleic acid comprises a polynucleotide of SEQ ID NO: 6, a polynucleotide hybridizable to SEQ ID NO: 6, a polynucleotide that encodes the polypeptide of SEQ ID NO: 7, a polynucleotide that encodes a polypeptide of SEQ ID NO: 12, a polynucleotide that encodes a polypeptide epitope of SEQ ID NO: 7, or a polynucleotide that is hybridizable to a polynucleotide that encodes a polypeptide epitope of SEQ ID NO: 7. Vectors and recombinant cells comprising the nucleic acid are also provided.

Abstract translation: 本发明提供编码人色氨酰-tRNA合成酶的水溶性多肽片段的分离的核酸，其可用于抑制血管发生。 核酸包含SEQ ID NO：6的多核苷酸，可与SEQ ID NO：6杂交的多核苷酸，编码SEQ ID NO：7的多肽的多核苷酸，编码SEQ ID NO：12的多肽的多核苷酸， 编码SEQ ID NO：7的多肽表位的多核苷酸或可与编码SEQ ID NO：7的多肽表位的多核苷酸杂交的多核苷酸。还提供了载体和包含该核酸的重组细胞。

公开(公告)号：US06903189B2

公开(公告)日：2005-06-07

申请号：US09813718

申请日：2001-03-21

Applicant: Paul Schimmel ,  Keisuke Wakasugi

Inventor： Paul Schimmel ,  Keisuke Wakasugi

IPC: A61K38/00 ,  C12N9/00 ,  C07K1/00 ,  A01N37/18 ,  C12P21/06

CPC classification number: C12N9/93 ,  A61K38/00

Abstract: The invention provides compositions comprising truncated tryptophanyl tRNA synthetase polypeptides useful for regulating angiogenesis, as well as nucleic acids encoding such truncated tRNA synthetase polypeptides. Methods of making and using such compositions are also disclosed.

Abstract translation: 本发明提供了包含用于调节血管发生的截短的色氨酰tRNA合成酶多肽的组合物，以及编码这种截短的tRNA合成酶多肽的核酸。 还公开了制备和使用这些组合物的方法。