公开(公告)号：US4580059A

公开(公告)日：1986-04-01

申请号：US617818

申请日：1984-06-06

申请人： Otto S. Wolfbeis ,  Edmund Urbano

发明人： Otto S. Wolfbeis ,  Edmund Urbano

IPC分类号： G01N33/52 ,  G01N21/64 ,  G01N21/76 ,  G01N21/78 ,  G01N21/85 ,  G01N31/22 ,  G01N33/50

CPC分类号： G01N21/643 ,  G01N2021/6432 ,  G01N2021/6441 ,  G01N2021/7786 ,  G01N21/8507 ,  Y10T436/207497 ,  Y10T436/209163

摘要： For the simultaneous measurement of the concentrations of several substances a number of fluorescence measurements corresponding to the number of substances to be tested are performed using at least one fluorescent indicator which is non-specific relative to at least one of the substances to be tested, each fluorescent indicator having different quenching constants with regard to the individual substances quenching its intensities.From the known, unquenched fluorescence intensities of the fluorescent indicators employed, the quenched fluroescence intensities obtained by measuring, and from the quenching constants that are known, or rather, have been determined beforehand by graphical methods or calculation, the concentrations and/or concentration ratios of the individual substances are obtained.

摘要翻译： 为了同时测量几种物质的浓度，使用至少一种相对于至少一种待测试物质是非特异性的至少一种荧光指示剂来进行对应于待测试物质数量的许多荧光测量，每个 荧光指示剂对于单个物质淬灭其强度具有不同的淬灭常数。 从所采用的荧光指示剂的已知的未熄灭的荧光强度，通过测量已知的或相当于已经通过图形方法或计算预先确定的淬灭常数获得的淬灭的荧光强度，浓度和/或浓度比 的各种物质。

公开(公告)号：US06911179B2

公开(公告)日：2005-06-28

申请号：US10189171

申请日：2002-07-05

申请人： Masanori Ando ,  Otto S. Wolfbeis ,  Vladimir M. Mirsky

发明人： Masanori Ando ,  Otto S. Wolfbeis ,  Vladimir M. Mirsky

IPC分类号： G01N31/00 ,  C08G73/00 ,  C08K3/04 ,  C08K3/22 ,  C08L79/08 ,  C08L101/00 ,  G01N21/78 ,  G01N27/12 ,  G01N31/22 ,  G01N33/00 ,  G01N27/02

CPC分类号： G01N33/0039 ,  Y02A50/247 ,  Y10T436/206664 ,  Y10T436/25875

摘要： The present invention provides an ozone detecting material comprising a polymer prepared by polymerization of at least one monomer selected from the group consisting of 2-, 3-, 4-halogen substituted anilines or aniline substituted with C1-3-alkyl in N-position, and diphenylamine, formed into a thin film coated on a transparent substrate, waveguide, or optical fiber, or additionally comprising a component selected from electro-conductive fine carbon powder, a semiconductive metal oxide, or a semiconductive polymer material, and a method for detecting ozone comprising the steps of contacting this ozone detecting material with an ozone-containing gas and determining the light absorption rate and/or electrical conductivity of the ozone detecting material at that time.

摘要翻译： 本发明提供一种臭氧检测材料，其包含通过聚合至少一种选自2-，3-，4-卤素取代的苯胺或由C 1-3取代的苯胺的单体制备的聚合物 N-烷基，和二苯胺，形成为涂覆在透明基板，波导或光纤上的薄膜，或另外包含选自导电细碳粉，半导体金属氧化物或半导体的成分 聚合物材料和臭氧检测方法，包括以下步骤：使该臭氧检测材料与含臭氧气体接触并确定此时臭氧检测材料的光吸收率和/或电导率。

公开(公告)号：US5853669A

公开(公告)日：1998-12-29

申请号：US588767

申请日：1996-01-19

申请人： Otto S. Wolfbeis

发明人： Otto S. Wolfbeis

IPC分类号： G01N31/22 ,  G01N21/29 ,  G01N21/00 ,  G01N21/75 ,  G01N33/48

CPC分类号： G01N31/221 ,  Y10S436/805 ,  Y10S436/807

摘要： For the purpose of reversible optical indication of the pH of a sample, a hydrophilic accommodating layer is disposed on a hydrophobic mechanically stable support element, which layer contains the indicator dye proper in an immobilized form. This provides a sensor membrane which can be fabricated in a simple manner and is cost-effective, which has a rapid response time and can also be fabricated as a mass product to be used only once.

摘要翻译： 为了对样品的pH的可逆光学指示的目的，将亲水性容纳层设置在疏水性机械稳定的支撑元件上，该层包含固定形式的指示剂染料。 这提供了一种传感器膜，其可以以简单的方式制造并且成本有效，其具有快速的响应时间，并且还可以制造成仅使用一次的质量产品。

公开(公告)号：US5182214A

公开(公告)日：1993-01-26

申请号：US556268

申请日：1990-07-20

申请人： Manfred Kessler ,  Otto S. Wolfbeis

发明人： Manfred Kessler ,  Otto S. Wolfbeis

IPC分类号： G01N21/64 ,  G01N21/78 ,  G01N31/22 ,  G01N33/48 ,  G01N33/52 ,  G01N33/68

CPC分类号： G01N33/6839 ,  G01N31/22 ,  G01N33/52

摘要： An optical method for quantitative determination of human serum albumin (HSA) in biological liquids such as urine is based on the observation that the absorption and fluorescence of certain anionic cyanine dyes possessing a terminal cyano group and having the general formula ##STR1## are strongly affected by HSA. This approach is distinctly more sensitive than existing techniques, allowing the detection and quantization of micro-albuminuria (i.e. detection of HSA in the 1-10 mg per liter concentration range) at costs which are distinctly lower than those for existing immunological assays. It is also much more specific for HSA than existing assays based on dye binding. Because the dyes has long wave absorptions and emissions, use can be made of electro-optical components based on semiconductor technology.

摘要翻译： 用于尿液等生物液体中的人血清白蛋白（HSA）的定量测​​定的光学方法是基于具有末端氰基并具有通式（I）的某些阴离子花青染料的吸收和荧光的观察， 受HSA强烈影响。 这种方法比现有技术明显更加敏感，允许微量白蛋白尿的检测和量化（即以1-10mg / l浓度范围检测HSA），成本明显低于现有免疫测定法。 与现有基于染料结合的测定相比，HSA还比HSA更具体。 由于染料具有长波吸收和辐射，因此可以使用基于半导体技术的电光元件。

公开(公告)号：US6139798A

公开(公告)日：2000-10-31

申请号：US843296

申请日：1997-04-14

申请人： Ingo Klimant ,  Hellfried Karpf ,  Otto S. Wolfbeis

发明人： Ingo Klimant ,  Hellfried Karpf ,  Otto S. Wolfbeis

IPC分类号： G01N21/77 ,  G01N21/17 ,  G01N21/62 ,  G01N21/64 ,  G01N31/22

CPC分类号： G01N31/225 ,  G01N31/223 ,  Y10T436/207497

摘要： The sensor membrane of an optical sensor for detection of O.sub.2, H.sub.2 O.sub.2, SO.sub.2 or halogenated hydrocarbons in a sample contains an indicator substance that is homogeneously immobilized in the polymer matrix of the sensor membrane and is, at least indirectly, in contact with the sample, changing at least one of its optical properties upon a change of the parameter to be measured. The indicator substance contains an inorganic salt of a transition metal complex with .alpha.-diimine ligands. The indicator substance is homogeneously distributed in the polymer matrix, which essentially consists of at least one substance belonging to the group of cellulose derivatives, polystyrenes, polytetrahydrofuranes, or their respective derivatives.

摘要翻译： 用于检测样品中的O 2，H 2 O 2，SO 2或卤代烃的光学传感器的传感器膜包含均匀地固定在传感器膜的聚合物基质中的指示物质，并且至少间接地与样品接触， 在要测量的参数的变化时改变其光学特性中的至少一个。 指示物质含有与α-二亚胺配体的过渡金属络合物的无机盐。 指示剂物质均匀分布在聚合物基质中，其基本上由属于纤维素衍生物，聚苯乙烯，聚四氢呋喃或它们各自的衍生物的至少一种物质组成。

公开(公告)号：US5407829A

公开(公告)日：1995-04-18

申请号：US83330

申请日：1993-06-29

申请人： Otto S. Wolfbeis ,  Helmut List

发明人： Otto S. Wolfbeis ,  Helmut List

IPC分类号： G01N1/22 ,  G01N31/22 ,  G01N33/00 ,  G01N31/00

CPC分类号： G01N31/22 ,  G01N31/223 ,  G01N2001/2229 ,  G01N2033/0081

摘要： For quality control of packaged organic substances, preferably packaged foods and drugs, the materials to be examined are brought into contact with a planar optical sensor element which is applied on the inside of the wrapping and responds to a change in the gas composition in the gas space above the sample by a change in color or fluorescence. The change of one of the optical properties of the sensor element is detected visually or opto-electronically.

摘要翻译： 为了质量控制包装的有机物质，优选包装的食品和药物，待检查的材料与施加在包装内部的平面光学传感器元件接触，并响应于气体中的气体组成的变化 通过颜色或荧光的变化在样品之上的空间。 传感器元件的光学特性之一的变化被视觉或光电检测。

公开(公告)号：US5157262A

公开(公告)日：1992-10-20

申请号：US706004

申请日：1991-05-28

申请人： Hermann Marsoner ,  Herbert Kroneis ,  Hellfried Karpf ,  Otto S. Wolfbeis ,  Helmut List ,  Alfred Leitner

发明人： Hermann Marsoner ,  Herbert Kroneis ,  Hellfried Karpf ,  Otto S. Wolfbeis ,  Helmut List ,  Alfred Leitner

IPC分类号： G01N21/64 ,  G01N21/25 ,  G01N21/27 ,  G01N21/77 ,  G01N21/81

CPC分类号： G01N21/77 ,  G01N21/255 ,  G01N2021/174 ,  G01N2021/7786 ,  G01N21/6428 ,  G01N21/81 ,  G01N2201/0621 ,  G01N2201/0622

摘要： Due to the size of the devices used for the excitation and measuring of light, conventional sensor elements for optically determining the concentrations of substances contained in gaseous and liquid samples, featuring an indicator layer with one or more indicator substances, are not well suited for use with microanalysis equipment, nor are they easy to mass-produce. These disadvantages are eliminated by integrating on the carrier layer (1) at least one photosensitive element (3) and its electric contact leads in planar arrangement, and by establishing optical contact between the indicator substance (7, 7') of the indicator layer (6) stimulated by the excitation radiation (11), and the photosensitive elements (3).

公开(公告)号：US5080865A

公开(公告)日：1992-01-14

申请号：US390754

申请日：1989-08-08

申请人： Marco J. Leiner ,  Karl Harnoncourt ,  Gerald Kirchmayer ,  Erich Kleinhappl ,  Helmut List ,  Hermann Marsoner ,  Otto S. Wolfbeis ,  Werner E. Ziegler

发明人： Marco J. Leiner ,  Karl Harnoncourt ,  Gerald Kirchmayer ,  Erich Kleinhappl ,  Helmut List ,  Hermann Marsoner ,  Otto S. Wolfbeis ,  Werner E. Ziegler

IPC分类号： G01N33/48 ,  G01N1/00 ,  G01N1/28 ,  G01N21/77 ,  G01N33/483 ,  G01N33/487 ,  G01N33/49

CPC分类号： G01N1/28 ,  G01N21/7703 ,  G01N2201/1288 ,  G01N33/487

摘要： In order to improve a one-way measuring element, which may be inserted into an analyzer for analyzing gaseous or liquid samples and which comprises a measuring channel with a measuring zone and one or more sensors located therein, the proposal is put forward that the one-way measuring element be provided with a sensor part whose measuring channel has seals on both ends, and with a sample-taking part, and that a coupling element be placed at the inlet end of the measuring channel for direct coupling of the sample-taking part containing the gaseous or liquid sample, and that the measuring channel be filled with a calibrating and storage medium prior to the measuring process, and that the calibrating and storage medium contained in the measuring channel be displaced by the sample flowing in after the sample-taking part has been coupled to the sensor part. The one-way measuring elements obtained in this way are characterized by great simplicity of design and ease of handling.

摘要翻译： 为了改进可以插入用于分析气体或液体样品的分析器中的单向测量元件，并且包括具有测量区域的测量通道和位于其中的一个或多个传感器，提出了一种方案 测量元件设有传感器部分，测量通道在两端都有密封，并具有采样部分，耦合元件放置在测量通道的入口端，用于直接耦合采样 包含气体或液体样品的部分，并且测量通道在测量过程之前用校准和存储介质填充，并且测量通道中包含的校准和存储介质被样品流动后的样品置换， 参与传感器部件。 以这种方式获得的单向测量元件的特征在于设计的简单性和易于操作。

公开(公告)号：US4716118A

公开(公告)日：1987-12-29

申请号：US898804

申请日：1986-08-21

申请人： Otto S. Wolfbeis ,  Helmut Offenbacher

发明人： Otto S. Wolfbeis ,  Helmut Offenbacher

IPC分类号： G01N21/80 ,  G01N21/64 ,  G01N21/77 ,  G01N33/52

CPC分类号： G01N21/6428 ,  G01N2021/6434 ,  G01N2021/7786 ,  G01N21/80

摘要： One measurement each is obtained from two optical sensors of ion concentration, following which the measured values are used for empirical determination of the ionic strength of an electrolytic solution. The sensors contain the same indicator substance but differ with regard to surface modification, thus ensuring different responses to ionic strength in spite of identical excitation and fluorescence wavelengths and the resulting simplicity of the optical system.

摘要翻译： 从两个离子浓度的光学传感器获得一个测量值，随后测量值用于经验测定电解液的离子强度。 传感器含有相同的指示剂物质，但是对于表面改性而言是不同的，因此尽管激发和荧光波长相同以及由此产生的光学系统的简单性，仍然确保了对离子强度的不同响应。

公开(公告)号：US06979575B1

公开(公告)日：2005-12-27

申请号：US10304365

申请日：2002-11-26

申请人： Nikolai N. Romanov ,  Otto S. Wolfbeis

发明人： Nikolai N. Romanov ,  Otto S. Wolfbeis

IPC分类号： C07D277/10 ,  C07D417/06 ,  C09B23/02 ,  G01N33/533 ,  G01N33/58 ,  C07D209/56 ,  C07K17/04 ,  C07K17/06 ,  G01N33/552

CPC分类号： C07D417/06 ,  C07D277/10 ,  C09B23/02 ,  G01N33/533 ,  G01N33/582

摘要： New reactive dyes are described which can be used to fluorescently label bioorganic molecules such as amino acids, proteins, antibodies, nucleotides and also polymer particles. The color of the new dyes and conjugates thereof can be varied over a wide range. In contrast to known symmetric cyanine dyes, the dyes of the invention contain only one reactive group. Hence the labelling takes place without the interfering cross-linking that occurs with bireactive dyes. Their fluorescence quantum yields are very high (especially in the conjugated form). Conjugates thereof with proteins, oligonucleotides or particles can be used in fluorescence-based analytical methods of determination e.g. in immunoassays, in hybridization assays, in cytometry or for pharmaceutical screening.

摘要翻译： 描述了可用于荧光标记生物有机分子如氨基酸，蛋白质，抗体，核苷酸以及聚合物颗粒的新的活性染料。 新染料及其结合物的颜色可以在很宽的范围内变化。 与已知的对称花青染料相反，本发明的染料仅含有一个反应性基团。 因此，标记发生在没有双重活性染料发生的干扰交联的情况下。 它们的荧光量子产率非常高（特别是在共轭形式）。 与蛋白质，寡核苷酸或颗粒的缀合物可以用于基于荧光的分析测定方法，例如 免疫测定，杂交测定，细胞计数或药物筛选。