公开(公告)号：US5308581A

公开(公告)日：1994-05-03

申请号：US916154

申请日：1992-07-31

申请人： Max Lippitsch ,  Marco J. Leiner ,  Hellfried Karpf

发明人： Max Lippitsch ,  Marco J. Leiner ,  Hellfried Karpf

IPC分类号： G01N21/64 ,  G01N21/78 ,  G01N21/80 ,  G01N31/22 ,  G02B6/00 ,  G01N33/94

CPC分类号： G01N31/221 ,  G01N21/6408 ,  G01N21/6428 ,  G01N21/6445

摘要： An indicator for use in a luminescence-optical configuration for continuous and reversible measurement of the pH of a sample within a relevant measuring range (M), whose luminescence decay time .tau. is dependent on the pH of the sample, is characterized by the following properties. The pK.sub.a value of the acidic or basic indicator is in the relevant measuring range when the indicator is in its electronic ground state (S.sub.0). The protonation rate k.sub.p of the base, or deprotonation rate k.sub.dp of the acid in the excited state (S.sub.1, X) of the indicator is lower than the decay rate k.sub.A of the excited state, and the luminescence decay times .tau..sub.S and .tau..sub.B of the acidic forms (AH*, BH.sup.+*) and the basic forms (A.sup.-*, B*) of the indicator are different from each other. If the pK.sub.a .tau. value of the indicator in its electronically excited state (S.sub.1, X) is within the relevant measuring range, the protonation rate k.sub.p of the base, or deprotonation rate k.sub.dp of the acid in the excited state (S.sub.1, X) of the indicator is higher than the decay rate k.sub.A of the excited state.

摘要翻译： PCT No.PCT / AT91 / 00128 Sec。 371日期：1992年7月31日 102（e）日期1992年7月31日PCT 1991年12月12日PCT PCT。 第WO92 / 10739号公报 日期1992年6月25日。一种用于发光 - 光学配置的指示剂，用于在相关测量范围（M）内连续和可逆测量样品的pH值，其发光衰减时间τ取决于样品的pH ，其特征在于以下性质。 当指示器处于电子基准状态（S0）时，酸性或碱性指示器的pKa值在相关测量范围内。 指示剂的激发态（S1，X）的酸的质子化速率kp或酸的去质子化速率kdp低于激发态的衰变速率kA，并且发光衰减时间τS和τB 指示剂的酸性形式（AH *，BH + *）和基本形式（A- *，B *）彼此不同。 如果其电子激发态（S1，X）中的指示剂的pKaτ值在相关测量范围内，则碱的质子化速率kp或激发态酸（S1，X）的去质子化速率kdp 该指标高于激发态的衰减速率kA。

公开(公告)号：US5080865A

公开(公告)日：1992-01-14

申请号：US390754

申请日：1989-08-08

申请人： Marco J. Leiner ,  Karl Harnoncourt ,  Gerald Kirchmayer ,  Erich Kleinhappl ,  Helmut List ,  Hermann Marsoner ,  Otto S. Wolfbeis ,  Werner E. Ziegler

发明人： Marco J. Leiner ,  Karl Harnoncourt ,  Gerald Kirchmayer ,  Erich Kleinhappl ,  Helmut List ,  Hermann Marsoner ,  Otto S. Wolfbeis ,  Werner E. Ziegler

IPC分类号： G01N33/48 ,  G01N1/00 ,  G01N1/28 ,  G01N21/77 ,  G01N33/483 ,  G01N33/487 ,  G01N33/49

CPC分类号： G01N1/28 ,  G01N21/7703 ,  G01N2201/1288 ,  G01N33/487

摘要： In order to improve a one-way measuring element, which may be inserted into an analyzer for analyzing gaseous or liquid samples and which comprises a measuring channel with a measuring zone and one or more sensors located therein, the proposal is put forward that the one-way measuring element be provided with a sensor part whose measuring channel has seals on both ends, and with a sample-taking part, and that a coupling element be placed at the inlet end of the measuring channel for direct coupling of the sample-taking part containing the gaseous or liquid sample, and that the measuring channel be filled with a calibrating and storage medium prior to the measuring process, and that the calibrating and storage medium contained in the measuring channel be displaced by the sample flowing in after the sample-taking part has been coupled to the sensor part. The one-way measuring elements obtained in this way are characterized by great simplicity of design and ease of handling.

摘要翻译： 为了改进可以插入用于分析气体或液体样品的分析器中的单向测量元件，并且包括具有测量区域的测量通道和位于其中的一个或多个传感器，提出了一种方案 测量元件设有传感器部分，测量通道在两端都有密封，并具有采样部分，耦合元件放置在测量通道的入口端，用于直接耦合采样 包含气体或液体样品的部分，并且测量通道在测量过程之前用校准和存储介质填充，并且测量通道中包含的校准和存储介质被样品流动后的样品置换， 参与传感器部件。 以这种方式获得的单向测量元件的特征在于设计的简单性和易于操作。

公开(公告)号：US5114676A

公开(公告)日：1992-05-19

申请号：US386151

申请日：1989-07-28

申请人： Marco J. Leiner ,  Leonie Weiss ,  Otto S. Wolfbeis

发明人： Marco J. Leiner ,  Leonie Weiss ,  Otto S. Wolfbeis

IPC分类号： C12Q1/00 ,  G01N21/64 ,  G01N21/77 ,  G01N21/78 ,  G01N21/80 ,  G01N31/22

CPC分类号： G01N21/643 ,  C12Q1/001 ,  G01N31/223 ,  G01N2021/6434 ,  G01N21/80 ,  Y10S435/817

摘要： In order to improve the response behavior of an optical sensor for determining at least one parameter in a liquid or gaseous sample, the sensor including a substrate which is provided with carrier particles and a fluorescent indicator immobilized thereon, the substrate is configured as a polymer film transparent to both excitation and emission radiation, and the individual carrier particles carrying the fluorescent indicator in immobilized form are bonded with only part of their surface to a thin layer of a thermoplastic material adhering to the polymer film and assume thermosetting properties after the carrier particles have been pressed in, whereas the other part of the surface of the carrier particles extend into an optically transparent hydrogel layer which covers the thermoplastic layer and in which the carrier particles are anchored mechanically. Such sensors may easily be attached to the end of an optical waveguide and may be made in any size by a simple punching operation.

摘要翻译： 为了改善用于确定液体或气体样品中的至少一个参数的光学传感器的响应特性，传感器包括设置有载体颗粒的基板和固定在其上的荧光指示器，该基板被配置为聚合物膜 对于激发和发射辐射都是透明的，并且携带固定形式的荧光指示剂的单个载体颗粒仅与其一部分表面粘合到粘附到聚合物膜上的热塑性材料的薄层，并且在载体颗粒具有 而载体颗粒的表面的另一部分延伸到覆盖热塑性层的光学透明的水凝胶层中，其中载体颗粒机械地锚定。 这样的传感器可以容易地附着到光波导的端部，并且可以通过简单的冲压操作制成任何尺寸。

公开(公告)号：US5232858A

公开(公告)日：1993-08-03

申请号：US844938

申请日：1992-03-04

申请人： Otto S. Wolfbeis ,  Marco J. Leiner

发明人： Otto S. Wolfbeis ,  Marco J. Leiner

IPC分类号： G01N21/64 ,  G01N21/80

CPC分类号： G01N21/6408 ,  G01N21/6428 ,  G01N21/80 ,  Y10T436/22

摘要： A method for the quantitative determination of one or more chemical parameters of a sample medium employs a fluorescent agent which is not responsive to the parameter to be determined and which has an excitation spectrum and an emission spectrum, and a closely adjacent chromogenic agent which responds to the parameter of the sample medium to be determined by a change in its absorption spectrum the emission spectrum of the fluorescent agent at least partically overlapping the absorption spectrum of the chromogenic agent. The absorption maximum of chromogenic agent is shifted in accordance with the parameter to be determined, causing a reduction of fluorescence decay time t in the fluorescent agent, thereby enabling quantitative determination of the chemical parameter with improved long-term stability and infrequent calibrations.

摘要翻译： 用于定量测定样品介质的一种或多种化学参数的方法使用荧光剂，其不响应于待确定的参数并且具有激发光谱和发射光谱，以及紧密相邻的显色剂，其响应于 通过其吸收光谱的变化来确定样品介质的参数，荧光剂的发射光谱至少部分地与发色剂的吸收光谱重叠。 显色剂的吸收最大值根据要测定的参数移动，导致荧光剂中的荧光衰减时间t降低，从而能够定期测定化学参数，改善长期稳定性和不经常性的校准。

公开(公告)号：US5464587A

公开(公告)日：1995-11-07

申请号：US242974

申请日：1994-05-16

申请人： Max Lippitsch ,  Sonja Draxler ,  Marco J. Leiner

发明人： Max Lippitsch ,  Sonja Draxler ,  Marco J. Leiner

IPC分类号： G01N21/78 ,  G01N21/64 ,  G01N31/22 ,  G01N33/84

CPC分类号： G01N33/84 ,  G01N21/6408 ,  G01N31/22

摘要： A luminescence-optical indicator for determining the activity of alkali metals based on decay time includes at least three components that are chemically bound to one another, i.e., a fluorophor, a functional group with which the luminescence decay time of the fluorophor can be influenced, and a ionophor binding the alkali metal ions to be measured in a selective and reversible manner, the effect of the functional group on the decay time of the fluorophor being altered in dependence on the activity of the alkali metal ions.

摘要翻译： 用于基于衰变时间确定碱金属活性的发光 - 光学指示剂包括至少三种彼此化学结合的组分，即荧光体，可以影响荧光体的发光衰减时间的官能团， 和以选择性和可逆方式结合待测量的碱金属离子的离子交换体，根据碱金属离子的活性，官能团对荧光体的衰变时间的影响被改变。