公开(公告)号：US09915658B2

公开(公告)日：2018-03-13

申请号：US14982015

申请日：2015-12-29

申请人： ORTHO-CLINICAL DIAGNOSTICS, INC.

发明人： Jian Zheng ,  Jianping Yang

IPC分类号： G01N33/576 ,  G01N33/569 ,  G01N33/53

CPC分类号： G01N33/56983 ,  G01N33/5306 ,  G01N33/5767 ,  G01N2333/186 ,  G01N2469/10 ,  G01N2469/20

摘要： The present invention is directed to combination immunoassays, reagents and kits for simultaneous detection of HCV antigens and anti-HCV antibodies in a sample. The combination immunoassays of the present invention employ a non-ionic detergent that effectively exposes or releases the HCV core antigen from virions in a sample without interfering with the performance of other reagents such as the capture of anti-HCV antibodies by recombinant HCV antigens.

公开(公告)号：US20160109447A1

公开(公告)日：2016-04-21

申请号：US14982015

申请日：2015-12-29

申请人： ORTHO-CLINICAL DIAGNOSTICS, INC.

发明人： Jian Zheng ,  Jianping Yang

IPC分类号： G01N33/569 ,  G01N33/53

CPC分类号： G01N33/56983 ,  G01N33/5306 ,  G01N33/5767 ,  G01N2333/186 ,  G01N2469/10 ,  G01N2469/20

摘要： The present invention is directed to combination immunoassays, reagents and kits for simultaneous detection of HCV antigens and anti-HCV antibodies in a sample. The combination immunoassays of the present invention employ a non-ionic detergent that effectively exposes or releases the HCV core antigen from virions in a sample without interfering with the performance of other reagents such as the capture of anti-HCV antibodies by recombinant HCV antigens.

摘要翻译： 本发明涉及用于在样品中同时检测HCV抗原和抗HCV抗体的组合免疫测定，试剂和试剂盒。 本发明的组合免疫测定使用非离子洗涤剂，其有效地从样品中的病毒粒子暴露或释放HCV核心抗原，而不干扰其它试剂的性能，例如通过重组HCV抗原捕获抗HCV抗体。

公开(公告)号：US10125177B2

公开(公告)日：2018-11-13

申请号：US15865594

申请日：2018-01-09

申请人： Ortho-Clinical Diagnostics, Inc.

发明人： Jian Zheng ,  Renee Yura ,  Jianping Yang

IPC分类号： C07K14/20 ,  G01N33/571 ,  G01N33/569

摘要： A Treponema pallidum triplet antigen construct is disclosed which includes three Treponema pallidum antigens (TP15, TP17, and TP47), as well as a ten amino acid leader sequence (tag 261) and human copper zinc superoxide dismutase (hSOD). This construct is optimized for in vitro diagnosis of syphilis infection. Plasmids containing DNA encoding the triplet antigen, host cells, production methods, detection methods, and kits are also disclosed.

公开(公告)号：US09221884B2

公开(公告)日：2015-12-29

申请号：US14193530

申请日：2014-02-28

申请人： Ortho-Clinical Diagnostics, Inc.

发明人： Jian Zheng ,  Renee Yura ,  Jianping Yang

IPC分类号： C07K14/20 ,  G01N33/569 ,  G01N33/571

CPC分类号： C07K14/20 ,  C07K2319/00 ,  C07K2319/40 ,  G01N33/56911 ,  G01N33/571 ,  G01N2333/20 ,  G01N2800/26

摘要： A Treponema pallidum triplet antigen construct is disclosed which includes three Treponema pallidum antigens (TP15, TP17, and TP47), as well as a ten amino acid leader sequence (tag 261) and human copper zinc superoxide dismutase (hSOD). This construct is optimized for in vitro diagnosis of syphilis infection. Plasmids containing DNA encoding the triplet antigen, host cells, production methods, detection methods, and kits are also disclosed.

摘要翻译： 公开了一种梅毒螺旋体三联体抗原构建体，其包括三种梅毒螺旋体抗原（TP15，TP17和TP47），以及十个氨基酸前导序列（标签261）和人铜锌超氧化物歧化酶（hSOD）。 该结构针对梅毒感染的体外诊断进行了优化。 还公开了含有编码三重抗原抗原的DNA，宿主细胞，生产方法，检测方法和试剂盒的质粒。

公开(公告)号：US20140178907A1

公开(公告)日：2014-06-26

申请号：US14193530

申请日：2014-02-28

申请人： Ortho-Clinical Diagnostics, Inc.

发明人： Jian Zheng ,  Renee Yura ,  Jianping Yang

IPC分类号： C07K14/20 ,  G01N33/569

CPC分类号： C07K14/20 ,  C07K2319/00 ,  C07K2319/40 ,  G01N33/56911 ,  G01N33/571 ,  G01N2333/20 ,  G01N2800/26

摘要： A Treponema pallidum triplet antigen construct is disclosed which includes three Treponema pallidum antigens (TP15, TP17, and TP47), as well as a ten amino acid leader sequence (tag 261) and human copper zinc superoxide dismutase (hSOD). This construct is optimized for in vitro diagnosis of syphilis infection. Plasmids containing DNA encoding the triplet antigen, host cells, production methods, detection methods, and kits are also disclosed.

摘要翻译： 公开了一种梅毒螺旋体三联体抗原构建体，其包括三种梅毒螺旋体抗原（TP15，TP17和TP47），以及十个氨基酸前导序列（标签261）和人铜锌超氧化物歧化酶（hSOD）。 该结构针对梅毒感染的体外诊断进行了优化。 还公开了含有编码三重抗原抗原的DNA，宿主细胞，生产方法，检测方法和试剂盒的质粒。

公开(公告)号：US10041946B2

公开(公告)日：2018-08-07

申请号：US15879547

申请日：2018-01-25

申请人： ORTHO-CLINICAL DIAGNOSTICS, INC.

发明人： Jian Zheng ,  Jianping Yang

IPC分类号： G01N33/569 ,  G01N33/576 ,  G01N33/53

CPC分类号： G01N33/56983 ,  G01N33/5306 ,  G01N33/5767 ,  G01N2333/186 ,  G01N2469/10 ,  G01N2469/20

摘要： The present invention is directed to combination immunoassays, reagents and kits for simultaneous detection of HCV antigens and anti-HCV antibodies in a sample. The combination immunoassays of the present invention employ a non-ionic detergent that effectively exposes or releases the HCV core antigen from virions in a sample without interfering with the performance of other reagents such as the capture of anti-HCV antibodies by recombinant HCV antigens.

公开(公告)号：US09902755B2

公开(公告)日：2018-02-27

申请号：US14980200

申请日：2015-12-28

申请人： Ortho-Clinical Diagnostics, Inc.

发明人： Jian Zheng ,  Renee Yura ,  Jianping Yang

IPC分类号： C07K14/20 ,  G01N33/569 ,  G01N33/571

CPC分类号： C07K14/20 ,  C07K2319/00 ,  C07K2319/40 ,  G01N33/56911 ,  G01N33/571 ,  G01N2333/20 ,  G01N2800/26

摘要： A Treponema pallidum triplet antigen construct is disclosed which includes three Treponema pallidum antigens (TP15, TP17, and TP47), as well as a ten amino acid leader sequence (tag 261) and human copper zinc superoxide dismutase (hSOD). This construct is optimized for in vitro diagnosis of syphilis infection. Plasmids containing DNA encoding the triplet antigen, host cells, production methods, detection methods, and kits are also disclosed.

公开(公告)号：US20160115206A1

公开(公告)日：2016-04-28

申请号：US14980200

申请日：2015-12-28

申请人： Ortho-Clinical Diagnostics, Inc.

发明人： Jian Zheng ,  Renee Yura ,  Jianping Yang

IPC分类号： C07K14/20 ,  G01N33/571

CPC分类号： C07K14/20 ,  C07K2319/00 ,  C07K2319/40 ,  G01N33/56911 ,  G01N33/571 ,  G01N2333/20 ,  G01N2800/26

摘要： A Treponema pallidum triplet antigen construct is disclosed which includes three Treponema pallidum antigens (TP15, TP17, and TP47), as well as a ten amino acid leader sequence (tag 261) and human copper zinc superoxide dismutase (hSOD). This construct is optimized for in vitro diagnosis of syphilis infection. Plasmids containing DNA encoding the triplet antigen, host cells, production methods, detection methods, and kits are also disclosed.

摘要翻译： 公开了一种梅毒螺旋体三联体抗原构建体，其包括三种梅毒螺旋体抗原（TP15，TP17和TP47），以及十个氨基酸前导序列（标签261）和人铜锌超氧化物歧化酶（hSOD）。 该结构针对梅毒感染的体外诊断进行了优化。 还公开了含有编码三重抗原抗原的DNA，宿主细胞，生产方法，检测方法和试剂盒的质粒。