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    Methods and apparatus that increase sequencing-by-binding efficiency
    3.
    发明授权
    Methods and apparatus that increase sequencing-by-binding efficiency 有权

    公开(公告)号：US10161003B2

    公开(公告)日：2018-12-25

    申请号：US15922787

    申请日：2018-03-15

    Applicant: OMNIOME, INC.

    Inventor： Sean Stromberg ,  John Vieceli ,  Kandaswamy Vijayan ,  Arnold Oliphant

    IPC: C12Q1/68 ,  C12Q1/6874 ,  C12Q1/6853

    Abstract: A method of determining a nucleic acid sequence that includes steps of: (a) contacting a primed template nucleic acid with a series of mixtures for forming ternary complexes, wherein each of the mixtures includes a polymerase and nucleotide cognates for at least two different base types suspected of being present at the next template position of the template nucleic acid; (b) monitoring the next template position for ternary complexes formed by the series of mixtures, wherein a signal state indicates presence or absence of ternary complex formed at the next template position by each individual mixture, thereby determining a series of signal states that encodes a base call for the next template position; and (c) decoding the series of signal states to distinguish a correct base call for the next template position from an error in the base call.

    Methods and apparatus that increase sequencing-by-binding efficiency
    6.
    发明授权
    Methods and apparatus that increase sequencing-by-binding efficiency 有权

    公开(公告)号：US09951385B1

    公开(公告)日：2018-04-24

    申请号：US15712632

    申请日：2017-09-22

    Applicant: OMNIOME, INC.

    Inventor： Kandaswamy Vijayan ,  Arnold Oliphant

    IPC: C12Q1/68

    CPC classification number: C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6853 ,  C12Q1/6869 ,  C12Q2525/186 ,  C12Q2565/101

    Abstract: A method of nucleic acid detection including (a) contacting a primed template with a polymerase, nucleotide cognate of a first base type and nucleotide cognate of a second base type under ternary complex stabilizing conditions; (b) contacting the primed template nucleic acid with a polymerase, nucleotide cognate of the first base type and nucleotide cognate of a third base type under ternary complex stabilizing conditions; (c) examining products of (a) and (b) for signals produced by a ternary complex that includes the primed template nucleic acid, polymerase and next correct nucleotide, wherein signals for the product of (a) are ambiguous for the first and second base type, and signals for the product of (b) are ambiguous for the first and third base type; (d) disambiguating signals acquired in (c) to identify the next correct nucleotide.

    Serial formation of ternary complex species
    9.
    发明授权
    Serial formation of ternary complex species 有权

    公开(公告)号：US11421262B2

    公开(公告)日：2022-08-23

    申请号：US16518321

    申请日：2019-07-22

    Applicant: Omniome, Inc.

    Inventor： Brittany A. Rohrman ,  Denis Malyshev ,  Morassa Mohseni Middleton ,  Arnold Oliphant

    IPC: C12Q1/6806 ,  C12Q1/686 ,  C12Q1/6816

    Abstract: A method for identifying a nucleotide in a primed template nucleic acid, including the steps of (a) providing a vessel having a primed template nucleic acid, polymerase and a nucleotide cognate of a first base type; (b) examining the vessel for a stabilized ternary complex including the polymerase and the nucleotide cognate of the first base type bound at a base position of the primed template nucleic acid; (c) delivering a nucleotide cognate of a second base type to the vessel, whereby the vessel retains the primed template nucleic acid and the polymerase from step (b); (d) examining the vessel for a stabilized ternary complex including the polymerase and the nucleotide cognate of the second base type bound at the base position of the primed template nucleic acid; and (e) identifying the type of nucleotide at the base position of the primed template nucleic acid.

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