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    ALPHA-AMYLASE VARIANTS AND POLYNUCLEOTIDES ENCODING SAME
    2.
    发明申请
    ALPHA-AMYLASE VARIANTS AND POLYNUCLEOTIDES ENCODING SAME 有权

    公开(公告)号：US20210189364A1

    公开(公告)日：2021-06-24

    申请号：US16770797

    申请日：2018-12-07

    申请人： NOVOZYMES A/S

    发明人： Carsten Andersen ,  Vasudeva Prahlada Rao ,  Rakhi Saikia ,  Vivek Srivastava ,  Kendra Darlene Stallings

    IPC分类号： C12N9/28 ,  C12P19/14 ,  C12P19/02 ,  C13K1/06

    摘要： The present invention relates to alpha-amylase variants comprising a substitution at a position corresponding to position 188 and at least one further substitution at a position corresponding to position 242 or 279 or 275 of SEQ ID NO: 1, in particular one or more combinations of substitutions selected from the group consisting of E188P+S242Y, E188P+S242F, E188P+S242H, E188P+S242W, E188P+S242P, E188P+S242I, E188P+S242T, E188P+S242L, E188P+K279W, E188P+K279Y, E188P+K279F, E188P+K279H, E188P+K279I, E188P+K279L, E188P+K279D, E188P+K279M, E188P+K279S, E188P+K279T E188P+K279N, E188P+K279Q, E188P+K279V, E188P+K279A, E188P+N275F, E188P+N275Y, E188P+N275W, and E188P+N275H, wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to a parent alpha amylase selected from the group consisting of: SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 18, and SEQ ID NO: 27. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

    Lipase variants and polynucleotides encoding same
    7.
    发明授权
    Lipase variants and polynucleotides encoding same 有权

    公开(公告)号：US10358635B2

    公开(公告)日：2019-07-23

    申请号：US16012906

    申请日：2018-06-20

    申请人： NOVOZYMES A/S

    发明人： Padmavathi Balumuri ,  Rakhi Saikia ,  Allan Svendsen ,  Lone Baunsgaard

    IPC分类号： C12N9/20 ,  C12N15/00 ,  C12N1/20 ,  C07H21/04 ,  C11D3/386 ,  C12P21/06 ,  C12P21/04 ,  C12N15/09 ,  C12P7/64

    摘要： The present invention relates to a lipase variant of a parent lipase, which variant has lipase activity, at least 75% but less than 100% sequence identity to SEQ ID NO: 3 and comprises a substitution at one or more positions corresponding to positions 1; 2; 3; 4; 5; 6; 7; 9; 10; 11; 12; 16; 19; 30; 31; 34; 36; 37; 39; 40; 42; 44; 51; 52; 53; 54; 56; 58; 59; 70; 71; 72; 73; 83; 84; 86; 88; 90; 92; 93; 95; 96; 100; 101; 102; 104; 106; 109; 110; 112; 117; 119; 124; 125; 127; 128; 131; 132; 133; 134; 135; 137; 158; 159; 160; 161; 162; 163; 165; 166; 167; 168; 170; 181; 182; 183; 189; 190; 192; 194; 196; 202; 210; 211; 212; 220; 225; 227; 228; 229; 230; 231; 233; 237; 238; 239; 240; 242; 246; 247; 248; 252; 259; 262; 264; 269 of SEQ ID NO: 3. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.