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公开(公告)号：US09951299B2
公开(公告)日：2018-04-24
申请号：US15101275
申请日：2014-12-10
申请人： Novozymes A/S
IPC分类号： C12N9/18 , C11D3/386 , C11D11/00 , D06M16/00 , D06M101/32
CPC分类号： C11D3/38636 , C11D11/0017 , C12N9/18 , C12Y301/01074 , D06M16/003 , D06M2101/32 , D06M2200/35
摘要： The present invention relates to variants with cutinase activity of a parent cutinase, comprising an alteration at one or more (e.g. several) positions corresponding to positions: 36, 37, 40, 78, 90, 114, 150, 196, 216, or 217 of SEQ ID NO: 2, wherein the alteration is a substitution for positions 40, 78, 90, 114, 150, 196 and 216, and a deletion for positions 36, 37 and 217, and wherein the variant has at least 75% but less than 100% sequence identity to the mature polypeptide of SEQ ID NO: 2.The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and method for obtaining and methods of producing the variants. It also relates to compositions comprising the variant, and to methods for using the variant.
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公开(公告)号：US20220089979A1
公开(公告)日：2022-03-24
申请号：US17343376
申请日：2021-06-09
申请人： Novozymes A/S
发明人： Jens Erik Nielsen , Lars Anderson , Allan Svendsen , Rune Nygaard Monrad , Lars Giger , Vasudeva Prahlada Rao , Sohel Dalal , Santhosh Vasu Mepadam , Marie Thrysoe Kruse , Kasper D. Rand , Pernille F. Jensen
摘要： The present invention relates to endoglucanase variants and methods for obtaining endoglucanase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
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公开(公告)号：US20190390182A1
公开(公告)日：2019-12-26
申请号：US16540180
申请日：2019-08-14
申请人： NOVOZYMES A/S
发明人： Rakhi Saikia , Geetha Hiremath Mendez , Sohel Dalal , Padmavathi Balumuri , Santhosh Vasu Mepadam , Lone Baunsgaard , Jesper Vind , Garry Paul Gippert , Kim Borch
摘要： The present invention relates to a lipase variant of a parent lipase, which variant has lipase activity, at least 75% but less than 100% sequence identity to SEQ ID NO: 3 and comprises a substitution at one or more positions corresponding to positions 1; 2; 3; 4; 5; 6; 7; 9; 10; 11; 12; 13; 14; 15; 17; 18; 29; 30; 33; 34; 36; 37; 38; 40; 41; 43; 44; 45; 46; 47; 49; 50; 51; 52; 54; 55; 57; 58; 59; 60; 69; 70; 71; 72; 81; 82; 85; 86; 87; 89; 90; 91; 92; 94; 95; 96; 97; 99; 100; 101; 102; 103; 105; 108; 111; 115; 116; 118; 119; 120; 122; 123; 124; 126; 127; 128; 129; 130; 131; 132; 133; 134; 136; 144; 152; 155; 157; 158; 159; 161; 162; 163; 177; 178; 181; 182; 184; 185; 187; 189; 191; 196; 197; 199; 203; 205; 206; 207; 208; 209; 211; 215; 220; 221; 222; 223; 224; 226; 229; 230; 232; 235; 236; 240; 242; 243; 244; 247; 248; 252; 254; 255; 257; 260; 261; 262 of SEQ ID NO: 3. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
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公开(公告)号：US11795418B2
公开(公告)日：2023-10-24
申请号：US17343376
申请日：2021-06-09
申请人： Novozymes A/S
发明人： Jens Erik Nielsen , Lars Anderson , Allan Svendsen , Rune Nygaard Monrad , Lars Giger , Vasudeva Prahlada Rao , Sohel Dalal , Santhosh Vasu Mepadam , Marie Thrysoe Kruse , Kasper D. Rand , Pernille F. Jensen
CPC分类号： C11D3/38645 , C11D3/38636 , C12N9/2437 , C12Y302/01004
摘要： The present invention relates to endoglucanase variants and methods for obtaining endoglucanase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
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公开(公告)号：US10822598B2
公开(公告)日：2020-11-03
申请号：US15742095
申请日：2016-07-06
申请人： NOVOZYMES A/S
发明人： Rakhi Saikia , Geetha Hiremath Mendez , Sohel Dalal , Padmavathi Balmuri , Santhosh Vasu Mepadam , Lone Baunsgaard , Jesper Vind , Garry Paul Gippert , Kim Borch
摘要： The present invention relates to a lipase variant of a parent lipase, which variant has lipase activity, at least 75% but less than 100% sequence identity to SEQ ID NO: 3 and comprises a substitution at one or more positions corresponding to positions 1; 2; 3; 4; 5; 6; 7; 9; 10; 11; 12; 13; 14; 15; 17; 18; 29; 30; 33; 34; 36; 37; 38; 40; 41; 43; 44; 45; 46; 47; 49; 50; 5 51; 52; 54; 55; 57; 58; 59; 60; 69; 70; 71; 72; 81; 82; 85; 86; 87; 89; 90; 91; 92; 94; 95; 96; 97; 99; 100; 101; 102; 103; 105; 108; 111; 115; 116; 118; 119; 120; 122; 123; 124; 126; 127; 128; 129; 130; 131; 132; 133; 134; 136; 144; 152; 155; 157; 158; 159; 161; 162; 163; 177; 178; 181; 182; 184; 185; 187; 189; 191; 196; 197; 199; 203; 205; 206; 207; 208; 209; 211; 215; 220; 221; 222; 223; 224; 226; 229; 230; 232; 235; 236; 240; 242; 243; 244; 247; 248; 252; 254; 255; 257; 10 260; 261; 262 of SEQ ID NO: 3. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
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公开(公告)号：US20180371438A1
公开(公告)日：2018-12-27
申请号：US15742095
申请日：2016-07-06
申请人： NOVOZYMES A/S
发明人： Rakhi Saikia , Geetha Hiremath Mendez , Sohel Dalal , Padmavathi Balmuri , Santhosh Vasu Mepadam , Lone Baunsgaard , Jesper Vind , Garry Paul Gippert , Kim Borch
摘要： The present invention relates to a lipase variant of a parent lipase, which variant has lipase activity, at least 75% but less than 100% sequence identity to SEQ ID NO: 3 and comprises a substitution at one or more positions corresponding to positions 1; 2; 3; 4; 5; 6; 7; 9; 10; 11; 12; 13; 14; 15; 17; 18; 29; 30; 33; 34; 36; 37; 38; 40; 41; 43; 44; 45; 46; 47; 49; 50; 5 51; 52; 54; 55; 57; 58; 59; 60; 69; 70; 71; 72; 81; 82; 85; 86; 87; 89; 90; 91; 92; 94; 95; 96; 97; 99; 100; 101; 102; 103; 105; 108; 111; 115; 116; 118; 119; 120; 122; 123; 124; 126; 127; 128; 129; 130; 131; 132; 133; 134; 136; 144; 152; 155; 157; 158; 159; 161; 162; 163; 177; 178; 181; 182; 184; 185; 187; 189; 191; 196; 197; 199; 203; 205; 206; 207; 208; 209; 211; 215; 220; 221; 222; 223; 224; 226; 229; 230; 232; 235; 236; 240; 242; 243; 244; 247; 248; 252; 254; 255; 257; 10 260; 261; 262 of SEQ ID NO: 3. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
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公开(公告)号：US11072765B2
公开(公告)日：2021-07-27
申请号：US16327376
申请日：2017-08-24
申请人： NOVOZYMES A/S
发明人： Jens Erik Nielsen , Lars Anderson , Allan Svendsen , Rune Nygaard Monrad , Lars Giger , Vasudeva Prahlada Rao , Sohel Dalal , Santhosh Vasu Mepadam , Marie Thrysoe Kruse , Kasper D. Rand , Pernille F. Jensen
摘要： The present invention relates to endoglucanase variants and methods for obtaining endoglucanase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
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公开(公告)号：US10724019B2
公开(公告)日：2020-07-28
申请号：US16540180
申请日：2019-08-14
申请人： NOVOZYMES A/S
发明人： Rakhi Saikia , Geetha Hiremath Mendez , Sohel Dalal , Padmavathi Balumuri , Santhosh Vasu Mepadam , Lone Baunsgaard , Jesper Vind , Garry Paul Gippert , Kim Borch
摘要： The present invention relates to a lipase variant of a parent lipase, which variant has lipase activity, at least 75% but less than 100% sequence identity to SEQ ID NO: 3 and comprises a substitution at one or more positions corresponding to positions 1; 2; 3; 4; 5; 6; 7; 9; 10; 11; 12; 13; 14; 15; 17; 18; 29; 30; 33; 34; 36; 37; 38; 40; 41; 43; 44; 45; 46; 47; 49; 50; 51; 52; 54; 55; 57; 58; 59; 60; 69; 70; 71; 72; 81; 82; 85; 86; 87; 89; 90; 91; 92; 94; 95; 96; 97; 99; 100; 101; 102; 103; 105; 108; 111; 115; 116; 118; 119; 120; 122; 123; 124; 126; 127; 128; 129; 130; 131; 132; 133; 134; 136; 144; 152; 155; 157; 158; 159; 161; 162; 163; 177; 178; 181; 182; 184; 185; 187; 189; 191; 196; 197; 199; 203; 205; 206; 207; 208; 209; 211; 215; 220; 221; 222; 223; 224; 226; 229; 230; 232; 235; 236; 240; 242; 243; 244; 247; 248; 252; 254; 255; 257; 260; 261; 262 of SEQ ID NO: 3. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
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公开(公告)号：US20190218478A1
公开(公告)日：2019-07-18
申请号：US16327376
申请日：2017-08-24
申请人： NOVOZYMES A/S
发明人： Jens Erik Nielsen , Lars Anderson , Allan Svendsen , Rune Nygaard Monrad , Lars Giger , Vasudeva Prahlada Rao , Sohel Dalal , Santhosh Vasu Mepadam , Marie Thrysoe Kruse , Kasper D. Rand , Pernille F. Jensen
CPC分类号： C11D3/38645 , C11D3/38636 , C12N9/2437 , C12Y302/01004
摘要： The present invention relates to endoglucanase variants and methods for obtaining endoglucanase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
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公开(公告)号：US20160355759A1
公开(公告)日：2016-12-08
申请号：US15101275
申请日：2014-12-10
申请人： NOVOZYMES A/S
CPC分类号： C11D3/38636 , C11D11/0017 , C12N9/18 , C12Y301/01074 , D06M16/003 , D06M2101/32 , D06M2200/35
摘要： Variants with cutinase activity of a parent cutinase, comprising an alteration at one or more (e.g. several) positions corresponding to positions: 181, 182, 115, 161, 1, 2, 43, 55, 79, or 5 of SEQ ID NO: 2, wherein the alteration is a substitution for positions 181, 115, 161, 43, 55, 79, and 5, and a deletion for positions 1, 2 and 182, and wherein the variant has at least 75% but less than 100% sequence identity to the mature polypeptide of SEQ ID NO: 2. Polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and method for obtaining and methods of producing the variants. Compositions comprising the variants and methods for using the variants.
摘要翻译： 包含亲本角质酶的角质酶活性的变体，包含对应于SEQ ID NO：1的位置：181,182,115,161,1,2,43,55,79或5的一个或多个（例如几个）位置的改变。 2，其中所述改变是位置181,151,161,43,55,79和5的替代，以及位置1,2和182的缺失，并且其中所述变体具有至少75％但小于100％ 与SEQ ID NO：2的成熟多肽的序列同一性。 核酸构建体，载体和包含多核苷酸的宿主细胞; 和获得的方法以及制备变体的方法。 包含变体的组合物和使用变体的方法。
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