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公开(公告)号：US10954477B2
公开(公告)日：2021-03-23
申请号：US15742076
申请日：2016-07-06
申请人： NOVOZYMES A/S
发明人： Jesper Vind , Lone Baunsgaard , Garry Paul Gippert
摘要： The present invention relates to reduced odor. More specifically the invention relates to a method for hydrolyzing a lipase substrate comprising: adding to said substrate a lipase variant of SEQ ID NO: 3, which variant comprises a substitution at one or more positions corresponding to positions 4; 44; 45; 49; 58; 84; 88; 93; 95; 97; 104; 108; 131; 140; 147; 152; 158; 165; 176; 177; 187; 195; 199; 208; 211; 221; 222; 224; 232; 244; 247; 248; 249; 251; 255; and 259 of SEQ ID NO: 3, has lipase activity, and has at least 75% but less than 100% sequence identity to SEQ ID NO: 3 or a fragment thereof with lipase activity, in which method odor is reduced when compared to the method wherein SEQ ID NO: 3 is added to the lipase substrate. The present invention also relates lipase variants, polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of producing and using the variants.
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公开(公告)号：US20210079318A1
公开(公告)日：2021-03-18
申请号：US17075185
申请日：2020-10-20
申请人： Novozymes A/S
发明人： Jesper Vind , Lone Baunsgaard , Garry Paul Gippert
摘要： The present invention relates to reduced odor. More specifically the invention relates to a method for hydrolyzing a lipase substrate comprising: adding to said substrate a lipase variant of SEQ ID NO: 3, which variant comprises a substitution at one or more positions corresponding to positions 4; 44; 45; 49; 58; 84; 88; 93; 95; 97; 104; 108; 131; 140; 147; 152; 158; 165; 176; 177; 187; 195; 199; 208; 211; 221; 222; 224; 232; 244; 247; 248; 249; 251; 255; and 259 of SEQ ID NO: 3, has lipase activity, and has at least 75% but less than 100% sequence identity to SEQ ID NO: 3 or a fragment thereof with lipase activity, in which method odor is reduced when compared to the method wherein SEQ ID NO: 3 is added to the lipase substrate. The present invention also relates lipase variants, polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of producing and using the variants.
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公开(公告)号：US20210123033A1
公开(公告)日：2021-04-29
申请号：US16962402
申请日：2019-02-08
申请人： Novozymes A/S
发明人： Jesper Vind , Kim Borch , Garry Paul Gippert
摘要： The present invention relates to a parent lipase and variants of a parent lipase. The invention also relates to polynucleotides encoding the lipases or lipase variants of the invention, compositions comprising lipase or lipase variants as well as methods of producing said lipases or lipase variants of the invention and the use of lipases, lipase variants or compositions thereof.
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公开(公告)号：US20210071157A1
公开(公告)日：2021-03-11
申请号：US16962425
申请日：2019-02-08
申请人： Novozymes A/S
发明人： Sune Fang Christensen , Allan Svendsen , Jesper Vind , Anders Gunnar Sandtrom , Thomas Agersten Poulsen , Alexander David Fulton , Carsten Hoerslev Hansen , Vibeke Skovgaard Nielsen , Kim Borch , Garry Paul Gippert
摘要： The present invention relates to lipase variants of the parent lipase shown as SEQ ID NO: 2 with improved wash performance and/or Benefit Risk Factor (BRF). The invention also relates to polynucleotides encoding the lipase variants of the invention, compositions comprising a lipase variant of the invention as well as methods of producing such lipase variants of the invention and the use of the lipase variants or compositions thereof.
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公开(公告)号：US10822598B2
公开(公告)日：2020-11-03
申请号：US15742095
申请日：2016-07-06
申请人： NOVOZYMES A/S
发明人： Rakhi Saikia , Geetha Hiremath Mendez , Sohel Dalal , Padmavathi Balmuri , Santhosh Vasu Mepadam , Lone Baunsgaard , Jesper Vind , Garry Paul Gippert , Kim Borch
摘要： The present invention relates to a lipase variant of a parent lipase, which variant has lipase activity, at least 75% but less than 100% sequence identity to SEQ ID NO: 3 and comprises a substitution at one or more positions corresponding to positions 1; 2; 3; 4; 5; 6; 7; 9; 10; 11; 12; 13; 14; 15; 17; 18; 29; 30; 33; 34; 36; 37; 38; 40; 41; 43; 44; 45; 46; 47; 49; 50; 5 51; 52; 54; 55; 57; 58; 59; 60; 69; 70; 71; 72; 81; 82; 85; 86; 87; 89; 90; 91; 92; 94; 95; 96; 97; 99; 100; 101; 102; 103; 105; 108; 111; 115; 116; 118; 119; 120; 122; 123; 124; 126; 127; 128; 129; 130; 131; 132; 133; 134; 136; 144; 152; 155; 157; 158; 159; 161; 162; 163; 177; 178; 181; 182; 184; 185; 187; 189; 191; 196; 197; 199; 203; 205; 206; 207; 208; 209; 211; 215; 220; 221; 222; 223; 224; 226; 229; 230; 232; 235; 236; 240; 242; 243; 244; 247; 248; 252; 254; 255; 257; 10 260; 261; 262 of SEQ ID NO: 3. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
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公开(公告)号：US20180371438A1
公开(公告)日：2018-12-27
申请号：US15742095
申请日：2016-07-06
申请人： NOVOZYMES A/S
发明人： Rakhi Saikia , Geetha Hiremath Mendez , Sohel Dalal , Padmavathi Balmuri , Santhosh Vasu Mepadam , Lone Baunsgaard , Jesper Vind , Garry Paul Gippert , Kim Borch
摘要： The present invention relates to a lipase variant of a parent lipase, which variant has lipase activity, at least 75% but less than 100% sequence identity to SEQ ID NO: 3 and comprises a substitution at one or more positions corresponding to positions 1; 2; 3; 4; 5; 6; 7; 9; 10; 11; 12; 13; 14; 15; 17; 18; 29; 30; 33; 34; 36; 37; 38; 40; 41; 43; 44; 45; 46; 47; 49; 50; 5 51; 52; 54; 55; 57; 58; 59; 60; 69; 70; 71; 72; 81; 82; 85; 86; 87; 89; 90; 91; 92; 94; 95; 96; 97; 99; 100; 101; 102; 103; 105; 108; 111; 115; 116; 118; 119; 120; 122; 123; 124; 126; 127; 128; 129; 130; 131; 132; 133; 134; 136; 144; 152; 155; 157; 158; 159; 161; 162; 163; 177; 178; 181; 182; 184; 185; 187; 189; 191; 196; 197; 199; 203; 205; 206; 207; 208; 209; 211; 215; 220; 221; 222; 223; 224; 226; 229; 230; 232; 235; 236; 240; 242; 243; 244; 247; 248; 252; 254; 255; 257; 10 260; 261; 262 of SEQ ID NO: 3. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
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公开(公告)号：US20210071156A1
公开(公告)日：2021-03-11
申请号：US16962421
申请日：2019-02-08
申请人： Novozymes A/S
发明人： Sune Fang Christensen , Allan Svendsen , Jesper Vind , Anders Gunnar Sandtrom , Thomas Agersten Poulsen , Alexander David Fulton , Carsten Hoerslev Hansen , Vibeke Skovgaard Nielsen , Kim Borch , Garry Paul Gippert
摘要： The present invention relates to lipase variants of the parent lipase shown as SEQ ID NO: 2 with improved stability during use in a wash process (IWS). The invention also relates to polynucleotides encoding the lipase variants of the invention, compositions comprising a lipase variant of the invention as well as methods of producing such lipase variants of the invention and the use of the lipase variants or compositions thereof.
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公开(公告)号：US20210071155A1
公开(公告)日：2021-03-11
申请号：US16962410
申请日：2019-02-08
申请人： Novozymes A/S
发明人： Sune Fang Christensen , Allan Svendsen , Jesper Vind , Anders Gunnar Sandtrom , Thomas Agersten Poulsen , Alexander David Fulton , Carsten Hoerslev Hansen , Vibeke Skovgaard Nielsen , Kim Borch , Garry Paul Gippert
摘要： The present invention relates to lipase variants of the parent lipase shown as SEQ ID NO: 2 with improved wash performance and/or Benefit Risk Factor (BRF). The invention also relates to polynucleotides encoding the lipase variants of the invention, compositions comprising a lipase variant of the invention as well as methods of producing such lipase variants of the invention and the use of the lipase variants or compositions thereof.
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公开(公告)号：US10724019B2
公开(公告)日：2020-07-28
申请号：US16540180
申请日：2019-08-14
申请人： NOVOZYMES A/S
发明人： Rakhi Saikia , Geetha Hiremath Mendez , Sohel Dalal , Padmavathi Balumuri , Santhosh Vasu Mepadam , Lone Baunsgaard , Jesper Vind , Garry Paul Gippert , Kim Borch
摘要： The present invention relates to a lipase variant of a parent lipase, which variant has lipase activity, at least 75% but less than 100% sequence identity to SEQ ID NO: 3 and comprises a substitution at one or more positions corresponding to positions 1; 2; 3; 4; 5; 6; 7; 9; 10; 11; 12; 13; 14; 15; 17; 18; 29; 30; 33; 34; 36; 37; 38; 40; 41; 43; 44; 45; 46; 47; 49; 50; 51; 52; 54; 55; 57; 58; 59; 60; 69; 70; 71; 72; 81; 82; 85; 86; 87; 89; 90; 91; 92; 94; 95; 96; 97; 99; 100; 101; 102; 103; 105; 108; 111; 115; 116; 118; 119; 120; 122; 123; 124; 126; 127; 128; 129; 130; 131; 132; 133; 134; 136; 144; 152; 155; 157; 158; 159; 161; 162; 163; 177; 178; 181; 182; 184; 185; 187; 189; 191; 196; 197; 199; 203; 205; 206; 207; 208; 209; 211; 215; 220; 221; 222; 223; 224; 226; 229; 230; 232; 235; 236; 240; 242; 243; 244; 247; 248; 252; 254; 255; 257; 260; 261; 262 of SEQ ID NO: 3. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
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公开(公告)号：US10662417B2
公开(公告)日：2020-05-26
申请号：US16308711
申请日：2017-07-05
申请人： NOVOZYMES A/S
发明人： Rune Nygaard Monrad , Peter Kamp Hansen , Frank Winther Rasmussen , Anne Vindum Due , Jens Erik Nielsen , Garry Paul Gippert
摘要： The present invention relates to pectate lyase variants exhibiting alterations relative to a parent enzyme exhibiting pectate lyase activity; to a method of producing such enzymes; and to methods for using such enzymes in the textile, detergent and cellulose fiber processing industries. Compared to the parent enzyme, the pectate lyase variants of the present invention exhibit improved stability in detergents.
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