公开(公告)号：US20170174746A1

公开(公告)日：2017-06-22

申请号：US15369294

申请日：2016-12-05

申请人： National Research Council of Canada

发明人： Subash Sad ,  Lakshmi Krishnan ,  Fanny Tzelepis ,  Valeria Alcon ,  Kevin G. Young

IPC分类号： C07K14/77 ,  A61K39/00

CPC分类号： C07K14/77 ,  A61K39/0005 ,  A61K39/0011 ,  A61K39/12 ,  A61K2039/522 ,  A61K2039/523 ,  C07K14/195 ,  C07K14/4713 ,  C07K14/4748 ,  C07K14/70596 ,  C07K14/71 ,  C07K2319/036 ,  C07K2319/35 ,  C12N1/20 ,  C12N9/0059 ,  C12N9/2462 ,  C12N9/6494 ,  C12N2760/10034 ,  C12Y110/03001 ,  C12Y304/24011 ,  Y02A50/41

摘要： The present invention relates to a recombinant bacterium expressing an antigen that is translocated to the cytosol of a host organism, and uses thereof. To this end, the present invention provides a recombinant bacterium comprising a nucleic acid encoding an antigen that is translocated to the cytosol of a host cell utilizing Type Ill secretion system. The recombinant bacterium is generally chosen from intracellular pathogens that reside in the phagosome and fail to induce rapid T cell activation. The translocated antigen may be a viral antigen, a bacterial antigen, or a tumour antigen. Methods of imparting immunity using the recombinant bacterium are also provided.

公开(公告)号：US09539313B2

公开(公告)日：2017-01-10

申请号：US14516090

申请日：2014-10-16

申请人： National Research Council of Canada

发明人： Subash Sad ,  Lakshmi Krishnan ,  Fanny Tzelepis ,  Valeria Alcon ,  Kevin G. Young

IPC分类号： A61K39/00 ,  A61K39/12 ,  C07K14/195 ,  C07K14/47 ,  C07K14/705 ,  C07K14/71 ,  C07K14/77 ,  C12N1/20 ,  C12N9/36 ,  C12N9/64 ,  C12N9/02

CPC分类号： C07K14/77 ,  A61K39/0005 ,  A61K39/0011 ,  A61K39/12 ,  A61K2039/522 ,  A61K2039/523 ,  C07K14/195 ,  C07K14/4713 ,  C07K14/4748 ,  C07K14/70596 ,  C07K14/71 ,  C07K2319/036 ,  C07K2319/35 ,  C12N1/20 ,  C12N9/0059 ,  C12N9/2462 ,  C12N9/6494 ,  C12N2760/10034 ,  C12Y110/03001 ,  C12Y304/24011 ,  Y02A50/41

摘要： The present invention relates to a recombinant bacterium expressing an antigen that is translocated to the cytosol of a host organism, and uses thereof. To this end, the present invention provides a recombinant bacterium comprising a nucleic acid encoding an antigen that is translocated to the cytosol of a host cell utilizing Type III secretion system. The recombinant bacterium is generally chosen from intracellular pathogens that reside in the phagosome and fail to induce rapid T cell activation. The translocated antigen may be a viral antigen, a bacterial antigen, or a tumor antigen. Methods of imparting immunity using the recombinant bacterium are also provided.

摘要翻译： 本发明涉及表达被转移到宿主生物的胞质溶胶中的抗原的重组细菌及其应用。 为此，本发明提供了一种重组细菌，其包含编码利用III型分泌系统转运至宿主细胞的胞质溶胶的抗原的核酸。 重组细菌通常选自存在于吞噬细胞中的细胞内病原体，并且不能诱导快速的T细胞活化。 易位抗原可以是病毒抗原，细菌抗原或肿瘤抗原。 还提供了使用重组细菌赋予免疫力的方法。