公开(公告)号：US11834647B2

公开(公告)日：2023-12-05

申请号：US17021158

申请日：2020-09-15

申请人： National Pingtung University of Science and Technology

发明人： Hso-Chi Chaung ,  Wen-Bin Chung ,  Ann Ying-An Chen ,  Mei-Li Wu

IPC分类号： C12N5/0783 ,  G01N33/50 ,  C12N5/0786 ,  G01N33/68 ,  A01K67/027

CPC分类号： C12N5/0638 ,  A01K67/0278 ,  C12N5/0637 ,  C12N5/0645 ,  C12N5/0646 ,  G01N33/5047 ,  G01N33/6869 ,  A01K2207/15 ,  A01K2217/206 ,  A01K2227/108 ,  A01K2267/0387

摘要： The present invention relates to an in vitro immune synapse system and a method of in vitro evaluating immune response using the same. The in vitro immune synapse system includes antigen-presenting cells (APCs) and at least one cell type of several specific T cell subtypes isolated from peripheral blood mononuclear cells (PBMCs), all of which is from a same individual of pigs. When a test sample is co-cultured in the in vitro immune synapse system for a given period, it can be determined that the test sample is immunogenic, immunostimulatory or not according to the immunization-related changes of these cells, thereby potentially replacing some kinds of animal experimentation.

公开(公告)号：US09441015B2

公开(公告)日：2016-09-13

申请号：US14667697

申请日：2015-03-25

申请人： NATIONAL PINGTUNG UNIVERSITY OF SCIENCE AND TECHNOLOGY

发明人： Mei-Li Wu ,  Hso-Chi Chaung ,  Guan-Ming Ke

IPC分类号： C07K14/005 ,  C12N7/00 ,  A61K39/39 ,  A61K39/12 ,  A61K39/00

CPC分类号： C07K14/005 ,  A61K39/12 ,  A61K2039/55561 ,  A61K2039/70 ,  C07K2319/00 ,  C12N2770/10022 ,  C12N2770/10034 ,  C12N2770/10071 ,  C12N2799/026

摘要： The present invention is directed to a recombinant fusion antigen gene, a recombinant fusion antigen protein and a subunit vaccine composition having the same against infection of porcine reproductive and respiratory syndrome virus (PRRSV). A recombinant fusion antigen gene, which encodes glycoprotein GP5 with truncated N′-terminal decoy epitope, a linker sequence and membrane protein M, followed by codon optimization, is expressed by a baculovirus expression system in vitro, thereby enhancing a yield of the recombinant fusion antigen protein. The recombinant fusion antigen protein can be applied in a subunit vaccine composition, for providing vaccinated animals with better protection ability without the risks of virulent spread and virulent recovery.

摘要翻译： 本发明涉及重组融合抗原基因，重组融合抗原蛋白和与猪繁殖与呼吸综合征病毒（PRRSV）感染相同的亚单位疫苗组合物。 编码具有截短的N末端诱饵表位的糖蛋白GP5，接头序列和膜蛋白M，然后密码子优化的重组融合抗原基因在体外由杆状病毒表达系统表达，从而提高重组融合物的产量 抗原蛋白。 重组融合抗原蛋白可以应用于亚单位疫苗组合物中，为疫苗接种的动物提供更好的保护能力，而不会产生毒性扩散和毒性恢复的风险。

公开(公告)号：US10117929B1

公开(公告)日：2018-11-06

申请号：US15821774

申请日：2017-11-23

申请人： National Pingtung University Of Science And Technology

发明人： Hso-Chi Chaung ,  Guan-Ming Ke ,  Mei-Li Wu

IPC分类号： A61K39/39 ,  A61K39/215 ,  C07H21/04 ,  C12N7/00 ,  A61K39/12 ,  A61K39/00

摘要： The present invention relates to a nucleic acid-based adjuvant and a porcine vaccine composition including the same. The nucleic acid-based adjuvant, which is a nucleic acid fragment consisting of a sequence listing as SEQ ID NO: 1, can be used as the nucleic acid-based adjuvant of a vaccine composition, thereby elevating the protective ability of the porcine vaccine composition.

公开(公告)号：US20150204854A1

公开(公告)日：2015-07-23

申请号：US14676915

申请日：2015-04-02

申请人： NATIONAL PINGTUNG UNIVERSITY OF SCIENCE AND TECHNOLOGY

发明人： Hso-Chi Chaung ,  Tsung-Hui Yang ,  Holi Chen ,  Chih-Cheng Chen ,  Hsien-Hsueh Chiu

IPC分类号： G01N33/50

CPC分类号： G01N33/5058 ,  A23L33/105 ,  A61K36/254 ,  A61K36/725 ,  G01N33/502 ,  G01N2333/415 ,  G01N2500/10 ,  G01N2510/00 ,  G01N2800/301

摘要： A Chinese herbal aqueous extract having anti-anxiety activities and a method of in vitro evaluating the same are disclosed. Neural cells and/or glial cells are cultured in a medium containing the Chinese herbal aqueous extract, and then subjected to an electric pulse treatment to form epileptic cells. The epileptic cells are applied on evaluation of at least one criterion of anti-anxiety activities of the Chinese herbal aqueous extract.

摘要翻译： 公开了具有抗焦虑活性的中草药含水提取物及其体外评价方法。 将神经细胞和/或神经胶质细胞在含有中草药提取物的培养基中培养，然后进行电脉冲处理以形成癫痫细胞。 应用癫痫细胞评价中草药提取物抗焦虑活性的至少一个标准。

公开(公告)号：US20240293536A1

公开(公告)日：2024-09-05

申请号：US18394532

申请日：2023-12-22

申请人： National Pingtung University Of Science And Technology

发明人： Hso-Chi Chaung ,  Wen-Bin Chung ,  Yen-Li Huang ,  Chi-Chih Chen ,  Yu-Chieh Chen

IPC分类号： A61K39/295 ,  A61K39/00 ,  A61K39/12 ,  A61P37/04

CPC分类号： A61K39/295 ,  A61K39/12 ,  A61P37/04 ,  A61K2039/552 ,  A61K2039/55561 ,  A61K2039/55566 ,  A61K2039/70

摘要： The present invention relates to a porcine bivalent subunit vaccine composition in a single dose. The porcine bivalent subunit vaccine composition includes porcine bivalent antigen, CpG adjuvant and a dual phase adjuvant. The porcine bivalent antigen consists of a classical swine fever virus (CSFV)-E2 recombinant protein and a porcine circovirus type 2 (PCV2)-ORF2 recombinant protein, both of which are produced by a mammalian cell expression system. The porcine bivalent subunit vaccine composition in a single dose can confer effectively immune protection against CSFV and PCV2 via a single vaccination without boost vaccination.

公开(公告)号：US20240209033A1

公开(公告)日：2024-06-27

申请号：US18485754

申请日：2023-10-12

申请人： National Pingtung University Of Science And Technology

发明人： Hso-Chi Chaung ,  Wen-Bin Chung ,  Chi-Chih Chen

IPC分类号： C07K14/005 ,  A61K39/00 ,  C12N5/071 ,  C12R1/91

CPC分类号： C07K14/005 ,  C12N5/0682 ,  A61K39/00 ,  C12N2770/24322 ,  C12N2770/24334 ,  C12R2001/91

摘要： The present disclosure relates to a mammalian cell line and a method for producing soluble E2 recombinant antigen of classical swine fever virus using the same. A mammalian cell expression system with different cell passage numbers can stably express a large amount of soluble CSFV-E2 recombinant protein, effectively reducing the production cost of CSFV-E2 recombinant protein, and then applied to the production of CSFV-E2 protein subunit vaccines.

公开(公告)号：US20230074637A1

公开(公告)日：2023-03-09

申请号：US17930168

申请日：2022-09-07

申请人： National Pingtung University of Science and Technology

发明人： Hso-Chi Chaung ,  Ko-Tung Chang ,  Mei-Li Wu ,  Wen-Bin Chung

IPC分类号： A61K39/12 ,  C12N15/85

摘要： The present invention relates to a recombinant antigen and an isolated polynucleotide of porcine reproductive and respiratory syndrome virus (PRRSV), a composition including the same and a method of making the same. The recombinant antigen is a chimeric protein of PRRSV dual structural proteins and T-cell epitope. The polynucleotide encodes an amino acid sequence of the recombinant antigen. The recombinant protein expressed by the polynucleotide in an eukaryotic expression system can be beneficial for mass production and purification. An immunogenic composition including the recombinant antigen can promote pro-inflammatory M1-phenotype polarization of porcine alveolar macrophages (PAMs), reduce receptor CD163 expression that is mediated for viral entry and activate T helper (Th1) immune responses, thereby being applied to a vaccine composition against PRRSV.

公开(公告)号：US09121848B2

公开(公告)日：2015-09-01

申请号：US14676915

申请日：2015-04-02

申请人： NATIONAL PINGTUNG UNIVERSITY OF SCIENCE AND TECHNOLOGY

发明人： Hso-Chi Chaung ,  Tsung-Hui Yang ,  Holi Chen ,  Chih-Cheng Chen ,  Hsien-Hsueh Chiu

IPC分类号： A61K36/00 ,  A61K36/254 ,  A61K36/725 ,  G01N33/50

CPC分类号： G01N33/5058 ,  A23L33/105 ,  A61K36/254 ,  A61K36/725 ,  G01N33/502 ,  G01N2333/415 ,  G01N2500/10 ,  G01N2510/00 ,  G01N2800/301

摘要： A Chinese herbal aqueous extract having anti-anxiety activities and a method of in vitro evaluating the same are disclosed. Neural cells and/or glial cells are cultured in a medium containing the Chinese herbal aqueous extract, and then subjected to an electric pulse treatment to form epileptic cells. The epileptic cells are applied on evaluation of at least one criterion of anti-anxiety activities of the Chinese herbal aqueous extract.

摘要翻译： 公开了具有抗焦虑活性的中草药含水提取物及其体外评价方法。 将神经细胞和/或神经胶质细胞在含有中草药提取物的培养基中培养，然后进行电脉冲处理以形成癫痫细胞。 应用癫痫细胞评价中草药提取物抗焦虑活性的至少一个标准。