公开(公告)号：US10793883B2

公开(公告)日：2020-10-06

申请号：US16123484

申请日：2018-09-06

Applicant: Morgan State University

Inventor： Viji Sitther ,  Somayeh Gharaie Fathabad

IPC: C12P7/64 ,  C07K14/195 ,  C12N1/20 ,  C12N15/74 ,  C12N9/00 ,  C12R1/01 ,  C12N9/02

Abstract: A recombinant strain of F. diplosiphon was made by transforming wild type F. diplosiphon with a pGEM-7Zf (+) plasmid containing sterol desaturase gene (SD) via electroporation. The recombinant strain was designated B481-SD and overexpressed the sterol desaturase gene to result in enhanced lipid production. Selection made on NaCl enabled growth of the transformant to thrive up to 50 g L−1 NaCl. This strain was designated B481-SDH.

公开(公告)号：US20240309407A1

公开(公告)日：2024-09-19

申请号：US18430193

申请日：2024-02-01

Applicant: Morgan State University

Inventor： Viji Sitther ,  Yavuz Yalcin ,  Busra Aydin

IPC: C12P7/6409 ,  C12N1/20 ,  C12P7/6427 ,  C12R1/01

CPC classification number: C12P7/6409 ,  C12N1/205 ,  C12P7/6427 ,  C12R2001/01

Abstract: Methods and compositions for increasing lipid production in cyanobacteria where the cyanobacteria is incubated in the presence of non-lethal amounts of antibiotic. Non-lethal doses of antibiotic weakens the cell membrane facilitating migration of fatty acids from the cell, reducing inhibition feedback of fatty acid precursor enzymes, leading to an increase in fatty acid production.

公开(公告)号：US20240099333A1

公开(公告)日：2024-03-28

申请号：US18371602

申请日：2023-09-22

Applicant: Morgan State University

Inventor： Ingrid Tulloch ,  Viji Sitther

IPC: A23K10/18 ,  A01K61/54 ,  A01K61/59 ,  A23K50/80

CPC classification number: A23K10/18 ,  A01K61/54 ,  A01K61/59 ,  A23K50/80

Abstract: A method for improving aquaculture growth and/or survival rate and hemocyte viability, comprising growing the aquaculture in the presence of cyanobacteria F. diplosiphon. The aquaculture may be a shellfish aquaculture, for example, shrimp, crayfish, oysters, clams, mussels, and crabs or combinations thereof. The aquaculture may be freshwater aquaculture, brackish aquaculture, or saltwater aquaculture. The F. diplosiphon may be a wild-type or an engineered/recombinant strain. The F. diplosiphon may be preferably added to aquacultures in amounts of 1 million colony forming units or more per ml of aquaculture volume.

公开(公告)号：US12024704B2

公开(公告)日：2024-07-02

申请号：US16712125

申请日：2019-12-12

Applicant: Morgan State University

Inventor： Viji Sitther ,  Somayeh Fathabad

IPC: C12N1/38 ,  C10L1/02 ,  C12N1/20 ,  C12P7/649

CPC classification number: C12N1/38 ,  C10L1/02 ,  C12N1/20 ,  C12P7/649 ,  C10L2200/0476 ,  C10L2290/26

Abstract: Compositions including metal nano- and/or micro-particles in solution with photosynthetic bioproduct producing microorganisms. These light harvesting complexes increase growth rates and photosynthetic efficiency of the constituent microorganisms, reducing the light required for a specific production level, or increases production for a specific light level. Compositions including zero-valent iron nanoparticles (nZVIs) in solution with photosynthetic bioproduct producing microorganisms. The nZVIs cause increased growth, lipid production and fatty acid production.

公开(公告)号：US20180291332A1

公开(公告)日：2018-10-11

申请号：US15833870

申请日：2017-12-06

Applicant: Morgan State University

Inventor： Viji Sitther ,  Behnam Tabatabai

IPC: C12N1/20 ,  C12R1/01 ,  C07K14/195 ,  C12P7/64

Abstract: The present invention relates to a recombinant cyanobacterium with enhanced halotolerance and compositions thereof, methods of producing the recombinant cyanobacterium, and methods of using the same for biofuel production. The invention also relates to transformed F. diplosiphon strains with enhanced salt tolerance.

公开(公告)号：US20230340502A1

公开(公告)日：2023-10-26

申请号：US17891482

申请日：2022-08-19

Applicant: Morgan State University

Inventor： Viji Sitther ,  Samson Gichuki

IPC: C12N15/74 ,  C12N1/20 ,  C12P7/649

CPC classification number: C12N15/74 ,  C12N1/205 ,  C12P7/649 ,  C12R2001/01

Abstract: A recombinant strain of F. diplosiphon was made by transforming wild type F. diplosiphon with a pGEM-7Zf (+) plasmid containing the photolyase gene (phrA) via electroporation. The recombinant strain was designated B481-ViAnSa and overexpressed the phrA gene to result in enhanced UV tolerance compared to wild type F. diplosiphon.

公开(公告)号：US11162067B2

公开(公告)日：2021-11-02

申请号：US15678643

申请日：2017-08-16

Applicant: Morgan State University

Inventor： Viji Sitther ,  Kadir Aslan ,  Behnam Tabatabai

IPC: C12N1/38 ,  C12P7/64 ,  C12N1/20 ,  C12P9/00 ,  C12P33/00 ,  C12P23/00 ,  C10L1/02 ,  C12N1/12

Abstract: Compositions including metal nano- and/or micro-particles in solution with photosynthetic bioproduct producing microorganisms. These light harvesting complexes increase growth rates and photosynthetic efficiency of the constituent microorganisms, reducing the light required for a specific production level, or increases production for a specific light level.

公开(公告)号：US20240352404A1

公开(公告)日：2024-10-24

申请号：US18758389

申请日：2024-06-28

Applicant: Morgan State University

Inventor： Viji Sitther ,  Somayeh Fathabad

IPC: C12N1/38 ,  C10L1/02 ,  C12N1/20 ,  C12P7/6458 ,  C12P7/649

CPC classification number: C12N1/38 ,  C10L1/02 ,  C12N1/20 ,  C12P7/6458 ,  C12P7/649 ,  C10L2200/0476 ,  C10L2290/26

Abstract: Compositions including metal nano-and/or micro-particles in solution with photosynthetic bioproduct producing microorganisms. These light harvesting complexes increase growth rates and photosynthetic efficiency of the constituent microorganisms, reducing the light required for a specific production level, or increases production for a specific light level. Compositions including zero-valent iron nanoparticles (nZVIs) in solution with photosynthetic bioproduct producing microorganisms. The nZVIs cause increased growth, lipid production and fatty acid production.

公开(公告)号：US20240327882A1

公开(公告)日：2024-10-03

申请号：US18592932

申请日：2024-03-01

Applicant: Morgan State University

Inventor： Viji Sitther ,  Yavuz Yalcin ,  Busra Aydin

IPC: C12P7/6409 ,  C12N1/20 ,  C12R1/01

CPC classification number: C12P7/6409 ,  C12N1/205 ,  C12R2001/01

Abstract: Methods and compositions are detailed for increasing bio-products in cyanobacteria where the cyanobacteria is incubated in the presence of non-lethal amounts of antibiotic. Non-lethal doses of antibiotic weaken the cell membrane facilitating migration of fatty acids from the cell, reducing inhibition feedback, leading to an increase in bio-products.

公开(公告)号：US20230392181A1

公开(公告)日：2023-12-07

申请号：US18329130

申请日：2023-06-05

Applicant: Morgan State University

Inventor： Viji Sitther ,  Samson Gichuki

IPC: C12Q1/02 ,  G01N33/573

CPC classification number: C12Q1/025 ,  G01N33/573 ,  G01N2333/90283

Abstract: Zero-valent iron nanoparticle-mediated oxidative stress was evaluated in F. diplosiphon as a measure of lipid peroxidation using malondialdehyde and thiobarbituric acid-reactivity (MDA-TBA) assay and the 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA) fluorogenic probe. Enzymatic response of nZVI-treated cultures was determined as a measure of FeSOD (iron superoxide dismutase) using western blotting and immunodetection. In addition, the distribution and surface assimilation of nZVIs was detected using transmission electron microscopy (TEM) and energy-dispersive X-ray spectroscopy (EDS).