公开(公告)号：US20230392181A1

公开(公告)日：2023-12-07

申请号：US18329130

申请日：2023-06-05

Applicant: Morgan State University

Inventor： Viji Sitther ,  Samson Gichuki

IPC: C12Q1/02 ,  G01N33/573

CPC classification number: C12Q1/025 ,  G01N33/573 ,  G01N2333/90283

Abstract: Zero-valent iron nanoparticle-mediated oxidative stress was evaluated in F. diplosiphon as a measure of lipid peroxidation using malondialdehyde and thiobarbituric acid-reactivity (MDA-TBA) assay and the 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA) fluorogenic probe. Enzymatic response of nZVI-treated cultures was determined as a measure of FeSOD (iron superoxide dismutase) using western blotting and immunodetection. In addition, the distribution and surface assimilation of nZVIs was detected using transmission electron microscopy (TEM) and energy-dispersive X-ray spectroscopy (EDS).

公开(公告)号：US20230340502A1

公开(公告)日：2023-10-26

申请号：US17891482

申请日：2022-08-19

Applicant: Morgan State University

Inventor： Viji Sitther ,  Samson Gichuki

IPC: C12N15/74 ,  C12N1/20 ,  C12P7/649

CPC classification number: C12N15/74 ,  C12N1/205 ,  C12P7/649 ,  C12R2001/01

Abstract: A recombinant strain of F. diplosiphon was made by transforming wild type F. diplosiphon with a pGEM-7Zf (+) plasmid containing the photolyase gene (phrA) via electroporation. The recombinant strain was designated B481-ViAnSa and overexpressed the phrA gene to result in enhanced UV tolerance compared to wild type F. diplosiphon.