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    Media solutions and methods for cryopreservation and thawing of in vitro fertilization specimens
    3.
    发明授权
    Media solutions and methods for cryopreservation and thawing of in vitro fertilization specimens 有权

    公开(公告)号：US08071281B2

    公开(公告)日：2011-12-06

    申请号：US12930776

    申请日：2011-02-07

    申请人： Michael D. Cecchi

    发明人： Michael D. Cecchi

    IPC分类号： A01N1/00 ,  C12N5/00

    CPC分类号： A01N1/0221 ,  A01N1/02

    摘要： A medium solution which will increase the growth, survival and ultimately the live birth rate of oocytes and embryos which have been or will be subjected to cryopreservation. The solution contains varied amounts of glucose, pyruvates, amino acids, vitamins K5 and C, antioxidants, fatty acids to supply the specimens with the chemical ingredients and uptake requirements required to recover and prosper during and after the cryopreservation process. The solution supplies nutrients to the specimens that will replenish depletion and damage to the specimens and their mitochondria, spindles and structural features, such as cell walls. One formulation addresses the additional requirements of frozen specimens as opposed to the current media solutions and methods which treat the un-frozen specimens the same as the frozen specimens when recovering them from cryopreservation.

    Media solutions and methods for cryopreservation and thawing of in vitro fertilization specimens
    4.
    发明申请
    Media solutions and methods for cryopreservation and thawing of in vitro fertilization specimens 有权

    公开(公告)号：US20110171625A1

    公开(公告)日：2011-07-14

    申请号：US12930776

    申请日：2011-02-07

    申请人： Michael D. Cecchi

    发明人： Michael D. Cecchi

    IPC分类号： A01N1/00

    CPC分类号： A01N1/0221 ,  A01N1/02

    摘要： A medium solution which will increase the growth, survival and ultimately the live birth rate of oocytes and embryos which have been or will be subjected to cryopreservation. The solution contains varied amounts of glucose, pyruvates, amino acids, vitamins K5 and C, antioxidants, fatty acids to supply the specimens with the chemical ingredients and uptake requirements required to recover and prosper during and after the cryopreservation process. The solution supplies nutrients to the specimens that will replenish depletion and damage to the specimens and their mitochondria, spindles and structural features, such as cell walls. One formulation addresses the additional requirements of frozen specimens as opposed to the current media solutions and methods which treat the un-frozen specimens the same as the frozen specimens when recovering them from cryopreservation.

    Lightweight chamber having variable configurations and a method for making such
    5.
    发明授权
    Lightweight chamber having variable configurations and a method for making such 失效
    具有可变构造的轻型腔室及其制造方法

    公开(公告)号：US07157270B2

    公开(公告)日：2007-01-02

    申请号：US10131632

    申请日：2002-04-24

    申请人： Michael D. Cecchi

    发明人： Michael D. Cecchi

    IPC分类号： C12M1/00

    CPC分类号： C12M41/14 ,  C12M23/48 ,  C12M41/12 ,  Y10S435/809

    摘要： An anabolic chamber constructed of a plastic with additives to create desirable characteristics such as resistance to corrosion and bacteria growth. The anabolic chamber has a double-wall structure with internal support ribs for creating a water jacket for facilitating maintaining temperature control within the chamber. Features such as handles, mounting blocks and surface detail are created integral to the anabolic chamber.

    摘要翻译： 由具有添加剂的塑料构成的合成代谢室，以产生所需的特性，例如耐腐蚀和细菌生长。 合成代谢室具有双壁结构，其具有用于产生水套的内部支撑肋，以便于在室内保持温度控制。 特征如手柄，安装块和表面细节是合成代谢室的一部分。

    Bladder system for controlling the temperature of laboratory fume hoods and working surfaces
    6.
    发明授权
    Bladder system for controlling the temperature of laboratory fume hoods and working surfaces 失效
    用于控制实验室通风橱和工作表面温度的膀胱系统

    公开(公告)号：US06734398B1

    公开(公告)日：2004-05-11

    申请号：US10352426

    申请日：2003-01-29

    申请人： Michael D. Cecchi

    发明人： Michael D. Cecchi

    IPC分类号： H05B100

    CPC分类号： B01L9/02 ,  B01L7/00 ,  B01L2300/185 ,  B08B15/023 ,  F28D2021/0077 ,  F28F3/12

    摘要： A heat transfer bladder system is incorporated into the interior of a laboratory work bench assembly and serves to control the temperature of a work surface of the work bench. The bladder contains a flowable media such as a fluid or gel which is heated or cooled so as to be able to control the temperature of the work bench work surface. The bladder system can include an external temperature control or an internal temperature control to control the temperature of the bladder fluid or gel. The bladder assembly may include through passages for adjunct instruments that can be used in conjunction with the work bench. The bladder can be formed from an elastomeric flexible material, or from a metal such as copper or stainless steel. The temperature control media can be circulated into and out of the bladder, or it can be contained in the bladder.

    摘要翻译： 传热气囊系统被并入实验室工作台组件的内部，并且用于控制工作台的工作表面的温度。 气囊包含可流动介质，例如流体或凝胶，其被加热或冷却，以便能够控制工作台工作表面的温度。 膀胱系统可以包括外部温度控制或内部温度控制以控制膀胱液或凝胶的温度。 气囊组件可以包括用于与工作台结合使用的辅助仪器的通路。 囊可以由弹性柔性材料形成，或者由金属如铜或不锈钢形成。 温度控制介质可以循环进入和离开膀胱，或者它可以包含在膀胱中。

    CONTROLLED ENVIRONMENT OBSERVATION DEVICE
    10.
    发明申请
    CONTROLLED ENVIRONMENT OBSERVATION DEVICE 审中-公开

    公开(公告)号：US20170146460A1

    公开(公告)日：2017-05-25

    申请号：US15352195

    申请日：2016-11-15

    申请人： Michael D. Cecchi ,  Monica Mezezi ,  Michael Ryan Cecchi ,  Aami Mezezi

    发明人： Michael D. Cecchi ,  Monica Mezezi ,  Michael Ryan Cecchi ,  Aami Mezezi

    IPC分类号： G01N21/84 ,  C12Q1/02

    CPC分类号： C12M41/36 ,  C12M21/06 ,  C12M23/22 ,  C12M41/12

    摘要： A controlled environment observation device comprising: a body; a heated shelf located in the body; a plurality of shelf openings in the heated shelf; a plurality of embryonic chambers removably installed in the plurality of shelf openings, each of the plurality of embryonic chambers comprising: an optically transparent heated shelf; a specimen dish removably attached to the top of the optically transparent heated shelf, the specimen dish having a generally optically transparent bottom; an x,y,z camera system located in each of the embryonic chambers and below the specimen dish and the optically transparent heated shelf; a computer located in the controlled environment observation device; and a controller in signal communication with the xyz camera system and the computer. A controlled environment observation device comprising: a body; at least one multi-area embryonic chamber located in the body, the multi-area embryonic chamber comprising: at least one outer wall forming a perimeter; a platform generally located within the perimeter; an opening in the platform; an optically transparent heated shelf located in the opening; a first plurality of inner walls forming a second plurality of volumes with the at least one outer wall; a second plurality of specimen dishes configured to be removeably attached to the optically transparent heated shelf in each of the second plurality of volumes, each of the specimen dishes having a generally optically transparent bottom; an x,y,z camera system located beneath the optically transparent heated shelf, and configured to move a camera to any of the specimen dishes.