公开(公告)号：US20020001546A1

公开(公告)日：2002-01-03

申请号：US09850123

申请日：2001-05-07

Applicant: Massachusetts Institute of Technology

Inventor： Ian Hunter ,  Colin J.H. Brenan ,  Tanya S. Kanigan

IPC: G01N021/00 ,  C12Q001/68 ,  C25B003/00 ,  B65B001/04 ,  C23C016/24

CPC classification number: B01L3/0293 ,  B01F13/0071 ,  B01F13/0084 ,  B01J19/0046 ,  B01J2219/00283 ,  B01J2219/00317 ,  B01J2219/00319 ,  B01J2219/00351 ,  B01J2219/0036 ,  B01J2219/00369 ,  B01J2219/00373 ,  B01J2219/00387 ,  B01J2219/00389 ,  B01J2219/00423 ,  B01J2219/0043 ,  B01J2219/00479 ,  B01J2219/00495 ,  B01J2219/005 ,  B01J2219/00511 ,  B01J2219/00533 ,  B01J2219/00536 ,  B01J2219/00549 ,  B01J2219/00585 ,  B01J2219/00587 ,  B01J2219/00596 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00686 ,  B01L3/0262 ,  B01L3/5025 ,  B01L3/50255 ,  B01L3/5085 ,  B01L3/50857 ,  B01L99/00 ,  B01L2200/0642 ,  B01L2300/0819 ,  B01L2300/0845 ,  B01L2400/022 ,  C40B60/14 ,  C40B70/00 ,  G01N30/466 ,  G01N30/6095 ,  G01N30/82 ,  G01N35/00069 ,  G01N2035/00237 ,  G01N2035/1037 ,  Y10T83/0481 ,  Y10T436/11 ,  Y10T436/25 ,  Y10T436/25625 ,  Y10T436/2575

Abstract: Methods for manufacturing and using an apparatus for manipulating and analyzing a large number of microscopic samples of a liquid or materials, including cells, in liquid suspension. Parallel through-holes are formed in a platen and loaded with a liquid. Loading may be performed in such a way as to create a gradient, with respect to the position of the through-holes, of the concentration of a particular substance or of another quantity. Mixing of the contents of through-holes may be obtained by bringing filled microwell arrays into contact with each other with registration of individual through-holes.

Abstract translation: 用于制造和使用用于操纵和分析液体或包括细胞在内的液体悬浮液的大量微观样品的装置的方法。 平行的通孔形成在压板中并装载液体。 可以以相对于通孔的位置产生特定物质的浓度或另一数量的浓度的梯度来进行装载。 可以通过使填充的微孔阵列通过单独的通孔对准而彼此接触来获得通孔内容物的混合。

公开(公告)号：US20040191924A1

公开(公告)日：2004-09-30

申请号：US10820679

申请日：2004-04-08

Applicant: Massachusetts Institute of Technology

Inventor： Ian Hunter ,  Colin J.H. Brenan ,  Tanya S. Kanigan

IPC: G01N001/10

CPC classification number: B01L3/0293 ,  B01F13/0071 ,  B01F13/0084 ,  B01J19/0046 ,  B01J2219/00283 ,  B01J2219/00317 ,  B01J2219/00319 ,  B01J2219/00351 ,  B01J2219/0036 ,  B01J2219/00369 ,  B01J2219/00373 ,  B01J2219/00387 ,  B01J2219/00389 ,  B01J2219/00423 ,  B01J2219/0043 ,  B01J2219/00479 ,  B01J2219/00495 ,  B01J2219/005 ,  B01J2219/00511 ,  B01J2219/00533 ,  B01J2219/00536 ,  B01J2219/00549 ,  B01J2219/00585 ,  B01J2219/00587 ,  B01J2219/00596 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00686 ,  B01L3/0262 ,  B01L3/5025 ,  B01L3/50255 ,  B01L3/5085 ,  B01L3/50857 ,  B01L9/523 ,  B01L2200/0642 ,  B01L2300/0819 ,  B01L2300/0829 ,  B01L2300/0845 ,  B01L2400/022 ,  C40B60/14 ,  C40B70/00 ,  G01N30/466 ,  G01N30/6095 ,  G01N30/82 ,  G01N35/00069 ,  G01N2035/00237 ,  G01N2035/1037 ,  Y10T83/0481 ,  Y10T436/11 ,  Y10T436/25 ,  Y10T436/25625 ,  Y10T436/2575

Abstract: Methods for loading a plurality of liquid samples into a through-hole array. Each transfer member of an array of transfer members is positioned for drawing a liquid sample from a distinct well of a microtiter plate. Liquid samples are drawn from wells of the microtiter plate and the array of transfer members is registered with a subset of through-holes of a through-hole array. The liquid samples are then dispensed from the transfer members into through-holes of the through-hole array.

Abstract translation: 将多个液体样品装载到通孔阵列中的方法。 转移构件阵列的每个转移构件被定位成从微量滴定板的不同孔中抽取液体样品。 液体样品从微量滴定板的孔中抽出，并且传送构件的阵列与通孔阵列的通孔的子集对齐。 然后将液体样品从转移构件分配到通孔阵列的通孔中。