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公开(公告)号：US20190381502A1
公开(公告)日：2019-12-19
申请号：US16426631
申请日：2019-05-30
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Michael C. Pallas , James C. Nurse , Gary Lim , Theodore E. Straub , Eliodor Ghenciu , Evan Foster , Jorge Fonseca , Kevin Maher
IPC: B01L3/00 , C12Q1/6806 , G03F7/20 , C12Q1/686
Abstract: A system for performing biological reactions is provided. The system includes a chip including a substrate and a plurality of reaction sites. The plurality of reaction sites are each configured to include a liquid sample of at most one nanoliter. Further, the system includes a control system configured to initiate biological reactions within the liquid samples. The system further includes a detection system configured to detect biological reactions on the chip. According to various embodiments, the chip includes at least 20000 reaction sites. In other embodiments, the chip includes at least 30000 reaction sites.
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2.发明授权公开(公告)号：US12275997B2
公开(公告)日：2025-04-15
申请号：US17976128
申请日：2022-10-28
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Gordon A. Janaway , Mark Andersen , Kornelija Zgonc , Michael C. Pallas , Marcin Sikora , Casey R. Mcfarland , Ferrier N. Le , Haopeng Wang , Jian Gong , Gothami Padmabandu
IPC: C12Q1/6886 , C12Q1/686 , G01N21/64
Abstract: Methods and systems for quantification of a target nucleic acid in a sample are provided. The method includes forming a plurality of discrete sample portions. Each of the plurality of discrete sample portions comprising a portion of the sample, and a reaction mixture. The method further includes amplifying the plurality of discrete sample portions to form a plurality of discrete processed sample portions. At least one discrete processed sample portion containing nucleic acid amplification reaction products. Fluorescence signals are detected from the at least one of the plurality of discrete processed sample portions to determine a presence of the at least one target nucleic acid. The method also includes determining the respective volumes of the plurality of the plurality of discrete processed sample portions, and estimating the number of copies-per-unit-volume of the at least one target nucleic acid in the sample. Estimating the number of copies-per-unit-volume is based on the number of discrete processed sample portions determined to contain the at least one target nucleic acid therein.
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3.发明授权公开(公告)号：US10557174B2
公开(公告)日：2020-02-11
申请号：US15080801
申请日：2016-03-25
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Gordon A. Janaway , Mark Andersen , Kornelija Zgonc , Michael C. Pallas , Marcin Sikora , Casey R. McFarland , Ferrier N. Le , Haopeng Wang , Jian Gong , Gothami Padmabandu
IPC: C12Q1/6886 , C12Q1/686 , G01N21/64
Abstract: Methods and systems for quantification of a target nucleic acid in a sample are provided. The method includes forming a plurality of discrete sample portions. Each of the plurality of discrete sample portions comprising a portion of the sample, and a reaction mixture. The method further includes amplifying the plurality of discrete sample portions to form a plurality of discrete processed sample portions. At least one discrete processed sample portion containing nucleic acid amplification reaction products. Fluorescence signals are detected from the at least one of the plurality of discrete processed sample portions to determine a presence of the at least one target nucleic acid. The method also includes determining the respective volumes of the plurality of the plurality of discrete processed sample portions, and estimating the number of copies-per-unit-volume of the at least one target nucleic acid in the sample. Estimating the number of copies-per-unit-volume is based on the number of discrete processed sample portions determined to contain the at least one target nucleic acid therein.
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4.发明申请SYSTEM AND METHOD FOR DETERMINING COPIES-PER-UNIT-VOLUME USING PCR AND FLOW CONTROL OF DROPLETS 审中-公开使用PCR和流量控制法确定复制单位体积的系统和方法公开(公告)号：US20160208342A1
公开(公告)日：2016-07-21
申请号：US15080801
申请日：2016-03-25
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Gordon A. Janaway , Mark Andersen , Kornelija Zgonc , Michael C. Pallas , Marcin Sikora , Casey R. McFarland , Ferrier N. Le , Haopeng Wang , Jian Gong , Gothami Padmabandu
CPC classification number: C12Q1/6886 , C12Q1/686 , C12Q2600/158 , C12Q2600/178 , G01N21/6486 , C12Q2537/16 , C12Q2563/143 , C12Q2563/159 , C12Q2565/626
Abstract: Methods and systems for quantification of a target nucleic acid in a sample are provided. The method includes forming a plurality of discrete sample portions. Each of the plurality of discrete sample portions comprising a portion of the sample, and a reaction mixture. The method further includes amplifying the plurality of discrete sample portions to form a plurality of discrete processed sample portions. At least one discrete processed sample portion containing nucleic acid amplification reaction products. Fluorescence signals are detected from the at least one of the plurality of discrete processed sample portions to determine a presence of the at least one target nucleic acid. The method also includes determining the respective volumes of the plurality of the plurality of discrete processed sample portions, and estimating the number of copies-per-unit-volume of the at least one target nucleic acid in the sample. Estimating the number of copies-per-unit-volume is based on the number of discrete processed sample portions determined to contain the at least one target nucleic acid therein.
Abstract translation: 提供了用于定量样品中靶核酸的方法和系统。 该方法包括形成多个离散的样本部分。 多个离散样品部分中的每一个包含样品的一部分和反应混合物。 该方法还包括放大多个离散样本部分以形成多个离散处理的样本部分。 至少一个离散加工的样品部分含有核酸扩增反应产物。 从所述多个离散处理样品部分中的至少一个检测荧光信号以确定所述至少一种靶核酸的存在。 该方法还包括确定多个多个离散处理的样本部分的相应体积，以及估计样品中至少一个靶核酸的每单位体积的拷贝数。 估计每单位体积的拷贝数是基于确定为在其中含有至少一种靶核酸的离散加工样品部分的数量。
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公开(公告)号：US20230201839A1
公开(公告)日：2023-06-29
申请号：US18086416
申请日：2022-12-21
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Michael C. Pallas , James C. Nurse , Gary Lim , Theodore E. Straub , Eliodor Ghenciu , Evan Foster , Jorge Fonseca , Kevin Maher
IPC: B01L3/00 , C12Q1/6806 , G03F7/20 , C12Q1/686
CPC classification number: B01L3/50857 , B01L3/50 , C12Q1/6806 , G03F7/2002 , B01L3/50851 , C12Q1/686 , B01L2300/0893 , B01L2300/0896 , B01L2300/161 , B01L2300/0654 , B01L2300/0829 , B01L2200/142 , B01L2300/046 , B01L2200/0642 , B01L7/52
Abstract: A system for performing biological reactions is provided. The system includes a chip including a substrate and a plurality of reaction sites. The plurality of reaction sites are each configured to include a liquid sample of at most one nanoliter. Further, the system includes a control system configured to initiate biological reactions within the liquid samples. The system further includes a detection system configured to detect biological reactions on the chip. According to various embodiments, the chip includes at least 20000 reaction sites. In other embodiments, the chip includes at least 30000 reaction sites.
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Patent Agency Ranking
公开(公告)号：US11590506B2
公开(公告)日：2023-02-28
申请号：US16426631
申请日：2019-05-30
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Michael C. Pallas , James C. Nurse , Gary Lim , Theodore E. Straub , Eliodor Ghenciu , Evan Foster , Jorge Fonseca , Kevin Maher
IPC: B01L3/00 , C12Q1/6806 , G03F7/20 , C12Q1/686 , B01L7/00
Abstract: A system for performing biological reactions is provided. The system includes a chip including a substrate and a plurality of reaction sites. The plurality of reaction sites are each configured to include a liquid sample of at most one nanoliter. Further, the system includes a control system configured to initiate biological reactions within the liquid samples. The system further includes a detection system configured to detect biological reactions on the chip. According to various embodiments, the chip includes at least 20000 reaction sites. In other embodiments, the chip includes at least 30000 reaction sites.
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公开(公告)号：US20230031117A1
公开(公告)日：2023-02-02
申请号：US17966364
申请日：2022-10-14
Inventor： Kuan Moon Boo , Wei Fuh Teo , Zeqi Tan , Soo Yong Lau , Ming Tiong Sia , Woon Liang Soh , Ching Yee Lam , Shan Hua Dong , Marc Haberstroh , Michael C. Pallas , Hardeep Sangha
Abstract: A system for biological analysis includes a housing, a block assembly within the housing having a sample block and a baseplate, a heated cover and a cover carrier. The sample block receives a sample holder comprising an RFID tag. A first drive mechanism generates relative movement between the sample block and the baseplate along a first axis. A second drive mechanism generates relative movement between the heated cover and the cover carrier along a second axis that is different from the first axis. Based on a first command the first drive mechanism releasably engages the sample block and operates the second drive mechanism to releasably engage the heated cover with the cover carrier. The system also includes first and second RFID antennas that receive RFID data from the sample holder RFID tag that is read by at least one RFID reader.
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公开(公告)号：US11493526B2
公开(公告)日：2022-11-08
申请号：US16852830
申请日：2020-04-20
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Eliodor Ghenciu , Theodore E. Straub , Evan W. Foster , Michael C. Pallas
Abstract: An apparatus for biological reactions is provided. The apparatus includes a substrate and a plurality of reaction sites within the substrate. A surface of the substrate is configured to have a first hydrophilicity and each surface of the plurality of reaction sites is configured to have a second hydrophilicity to load a substantial number of reaction sites with a sample volume. The sample volume of each loaded reaction site is substantially confined to its respective reaction site. The sample volume is configured to undergo a biological reaction within the reaction site.
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公开(公告)号：US11480584B2
公开(公告)日：2022-10-25
申请号：US16777820
申请日：2020-01-30
Inventor： Kuan Moon Boo , Wei Fuh Teo , Zeqi Tan , Soo Yong Lau , Ming Tiong Sia , Woon Liang Soh , Ching Yee Lam , Shan Hua Dong , Marc Haberstroh , Michael C. Pallas , Hardeep Sangha
Abstract: A system for biological analysis includes a housing, a block assembly within the housing having a sample block and a baseplate, a heated cover and a cover carrier. The sample block receives a sample holder comprising an RFID tag. A first drive mechanism generates relative movement between the sample block and the baseplate along a first axis. A second drive mechanism generates relative movement between the heated cover and the cover carrier along a second axis that is different from the first axis. Based on a first command the first drive mechanism releasably engages the sample block and operates the second drive mechanism to releasably engage the heated cover with the cover carrier. The system also includes first and second RFID antennas that receive RFID data from the sample holder RFID tag that is read by at least one RFID reader.
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公开(公告)号：US20150080247A1
公开(公告)日：2015-03-19
申请号：US14385730
申请日：2013-03-15
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Michael C. Pallas , James C. Nurse , Gary Lim , Theodore E. Straub , Eliodor Ghenciu , Evan W. Foster , Jorge Fonseca , Kevin Maher
CPC classification number: B01L3/50857 , B01L3/50 , B01L3/50851 , B01L7/52 , B01L2200/0642 , B01L2200/142 , B01L2300/046 , B01L2300/0654 , B01L2300/0829 , B01L2300/0893 , B01L2300/0896 , B01L2300/161 , C12Q1/6806 , C12Q1/686 , G03F7/2002
Abstract: A system for performing biological reactions is provided. The system includes a chip including a substrate and a plurality of reaction sites. The plurality of reaction sites are each configured to include a liquid sample of at most one nanoliter. Further, the system includes a control system configured to initiate biological reactions within the liquid samples. The system further includes a detection system configured to detect biological reactions on the chip. According to various embodiments, the chip includes at least 20000 reaction sites. In other embodiments, the chip includes at least 30000 reaction sites.
Abstract translation: 提供了用于进行生物反应的系统。 该系统包括包括基底和多个反应位点的芯片。 多个反应位点各自被配置为包括至多一个纳升级的液体样品。 此外，该系统包括被配置为启动液体样品内的生物反应的控制系统。 该系统还包括被配置为检测芯片上的生物反应的检测系统。 根据各种实施方案，芯片包括至少20000个反应位点。 在其它实施方案中，芯片包括至少30000个反应位点。
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