公开(公告)号：US10176277B2

公开(公告)日：2019-01-08

申请号：US14011190

申请日：2013-08-27

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Francis T. Cheng ,  Casey R. McFarland

IPC: G01K15/00 ,  G01K19/00 ,  G06F17/50 ,  C12Q1/6816 ,  G06F19/18

Abstract: Methods are provided that operate on raw dissociation data and dissociation curves to generate calibrations of the detected data and to further improve analysis of the data. The data can be taken from each support region of a multi-region platform, for example, from each well of a multi-well plate. Each support region can be loaded with portions of the same sample. In some embodiments, a dissociation curve correction can be calibrated for the sample, prior to a run of an experiment using such sample. In some embodiments, a method is provided for generating a melting transition region of dissociation curves that show the melting characteristics of the sample. In some embodiments, dye temperature dependence correction can be performed on the dissociation curve data to further improve analysis. In some embodiments, a feature vector can be derived from the melt data, and the feature vector can be used to further improve genotyping analysis of the dissociation curves.

公开(公告)号：US20140067345A1

公开(公告)日：2014-03-06

申请号：US14011190

申请日：2013-08-27

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Francis T. Cheng ,  Casey R. McFarland

IPC: G06F17/50

CPC classification number: G06F17/5009 ,  C12Q1/6816 ,  G06F19/18 ,  C12Q2527/107 ,  C12Q2537/165 ,  C12Q2539/101

Abstract: Methods are provided that operate on raw dissociation data and dissociation curves to generate calibrations of the detected data and to further improve analysis of the data. The data can be taken from each support region of a multi-region platform, for example, from each well of a multi-well plate. Each support region can be loaded with portions of the same sample. In some embodiments, a dissociation curve correction can be calibrated for the sample, prior to a run of an experiment using such sample. In some embodiments, a method is provided for generating a melting transition region of dissociation curves that show the melting characteristics of the sample. In some embodiments, dye temperature dependence correction can be performed on the dissociation curve data to further improve analysis. In some embodiments, a feature vector can be derived from the melt data, and the feature vector can be used to further improve genotyping analysis of the dissociation curves.

Abstract translation: 提供了对原始解离数据和解离曲线进行操作以产生检测数据的校准并进一步改进数据分析的方法。 可以从多区域平台的每个支撑区域（例如，从多孔板的每个孔）获取数据。 每个支撑区域可以加载相同样品的部分。 在一些实施方案中，在使用这种样品的实验运行之前，可以对样品校准解离曲线校正。 在一些实施方案中，提供了一种产生解析曲线的熔融转变区域的方法，其显示样品的熔融特性。 在一些实施方案中，可以对解离曲线数据进行染料温度依赖性校正，以进一步改进分析。 在一些实施例中，可以从熔体数据导出特征向量，并且可以使用特征向量来进一步改进解离曲线的基因分型分析。

公开(公告)号：US10557174B2

公开(公告)日：2020-02-11

申请号：US15080801

申请日：2016-03-25

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Gordon A. Janaway ,  Mark Andersen ,  Kornelija Zgonc ,  Michael C. Pallas ,  Marcin Sikora ,  Casey R. McFarland ,  Ferrier N. Le ,  Haopeng Wang ,  Jian Gong ,  Gothami Padmabandu

IPC: C12Q1/6886 ,  C12Q1/686 ,  G01N21/64

Abstract: Methods and systems for quantification of a target nucleic acid in a sample are provided. The method includes forming a plurality of discrete sample portions. Each of the plurality of discrete sample portions comprising a portion of the sample, and a reaction mixture. The method further includes amplifying the plurality of discrete sample portions to form a plurality of discrete processed sample portions. At least one discrete processed sample portion containing nucleic acid amplification reaction products. Fluorescence signals are detected from the at least one of the plurality of discrete processed sample portions to determine a presence of the at least one target nucleic acid. The method also includes determining the respective volumes of the plurality of the plurality of discrete processed sample portions, and estimating the number of copies-per-unit-volume of the at least one target nucleic acid in the sample. Estimating the number of copies-per-unit-volume is based on the number of discrete processed sample portions determined to contain the at least one target nucleic acid therein.

公开(公告)号：US20160208342A1

公开(公告)日：2016-07-21

申请号：US15080801

申请日：2016-03-25

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Gordon A. Janaway ,  Mark Andersen ,  Kornelija Zgonc ,  Michael C. Pallas ,  Marcin Sikora ,  Casey R. McFarland ,  Ferrier N. Le ,  Haopeng Wang ,  Jian Gong ,  Gothami Padmabandu

IPC: C12Q1/68 ,  G01N21/64

CPC classification number: C12Q1/6886 ,  C12Q1/686 ,  C12Q2600/158 ,  C12Q2600/178 ,  G01N21/6486 ,  C12Q2537/16 ,  C12Q2563/143 ,  C12Q2563/159 ,  C12Q2565/626

Abstract: Methods and systems for quantification of a target nucleic acid in a sample are provided. The method includes forming a plurality of discrete sample portions. Each of the plurality of discrete sample portions comprising a portion of the sample, and a reaction mixture. The method further includes amplifying the plurality of discrete sample portions to form a plurality of discrete processed sample portions. At least one discrete processed sample portion containing nucleic acid amplification reaction products. Fluorescence signals are detected from the at least one of the plurality of discrete processed sample portions to determine a presence of the at least one target nucleic acid. The method also includes determining the respective volumes of the plurality of the plurality of discrete processed sample portions, and estimating the number of copies-per-unit-volume of the at least one target nucleic acid in the sample. Estimating the number of copies-per-unit-volume is based on the number of discrete processed sample portions determined to contain the at least one target nucleic acid therein.

Abstract translation: 提供了用于定量样品中靶核酸的方法和系统。 该方法包括形成多个离散的样本部分。 多个离散样品部分中的每一个包含样品的一部分和反应混合物。 该方法还包括放大多个离散样本部分以形成多个离散处理的样本部分。 至少一个离散加工的样品部分含有核酸扩增反应产物。 从所述多个离散处理样品部分中的至少一个检测荧光信号以确定所述至少一种靶核酸的存在。 该方法还包括确定多个多个离散处理的样本部分的相应体积，以及估计样品中至少一个靶核酸的每单位体积的拷贝数。 估计每单位体积的拷贝数是基于确定为在其中含有至少一种靶核酸的离散加工样品部分的数量。