公开(公告)号：US20230222660A1

公开(公告)日：2023-07-13

申请号：US18152208

申请日：2023-01-10

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Soeren ALSHEIMER ,  Kai WALTER ,  Joachim BRADL

IPC: G06T7/00 ,  H04N13/207 ,  G02B21/36 ,  G01N33/50

CPC classification number: G06T7/0014 ,  H04N13/207 ,  G02B21/365 ,  G01N33/5094 ,  G06T2207/10056 ,  G06T2207/30024 ,  G06T2207/30096 ,  G06T2200/04 ,  G06T2207/30204

Abstract: A method for matching a three-dimensional first image of at least one discrete entity with a three-dimensional second image of the at least one discrete entity is provided. The at least one discrete entity includes a biological sample and a plurality of constituent parts of a marker. The method includes: generating a first representation of the marker from the first image; generating a second representation of the marker from the second image; and based upon the representations matching, matching the first image with the second image; or based upon the representations not matching, rejecting the match. Generating the representations includes determining vectors from at least one reference item to at least some of the constituent parts of the marker, determining for the vectors at least one value of a property, and generating the representations of the marker based on a frequency of the at least one value of the property.

公开(公告)号：US20240402475A1

公开(公告)日：2024-12-05

申请号：US18698024

申请日：2022-09-30

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Soeren ALSHEIMER ,  Joachim BRADL

IPC: G02B21/00 ,  G01N15/1434 ,  G02B21/26

Abstract: An imaging system for imaging a sample includes a sample moving unit configured to move the sample in a sample space along a movement direction. The imaging system further includes at least one detection optic including an optical axis enclosing a first angle with the movement direction within a range of 20° to 70°, the optical axis of the at least one detection optic and the movement direction defining a first plane; and at least one illumination optic including an optical axis that encloses a second angle with the movement direction within a range of 70° to 110°, and that encloses a third angle with the optical axis of the at least one detection optic within a range of 70° to 110°, the optical axis of the at least one illumination optic and the movement direction defining a second plane, the first and second planes intersecting and being different.

公开(公告)号：US20250052649A1

公开(公告)日：2025-02-13

申请号：US18796325

申请日：2024-08-07

Applicant: Leica Microsystems CMS GmbH

Inventor： Soeren ALSHEIMER ,  Joachim BRADL

IPC: G01N1/30 ,  G01N33/58

Abstract: A method for analysing a biological sample that includes multiple targets is provided. The method includes staining the targets of the biological sample with multiple detectable markers, providing affinity information specifying an affinity of each marker towards at least some of the targets, generating a readout of the biological sample with the markers to generate raw readout data, and unmixing the raw readout data by applying an unmixing algorithm and the affinity information to generate unmixed readout data with respect to the targets.

公开(公告)号：US20240159647A1

公开(公告)日：2024-05-16

申请号：US18284302

申请日：2021-05-04

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Soeren ALSHEIMER ,  Joachim BRADL

IPC: G01N15/1429 ,  G01N15/10 ,  G01N15/14 ,  G06V20/69

CPC classification number: G01N15/1429 ,  G01N15/147 ,  G06V20/693 ,  G06V20/695 ,  G06V20/698 ,  G01N2015/1006 ,  G01N2015/1493 ,  G01N2015/1497

Abstract: A method for optically recognizing at least one target discrete entity from a plurality of discrete entities includes acquiring a first optical read-out of a marker of a first discrete entity that defines the target discrete entity, generating a first set of representations of the marker of the first discrete entity based on the first optical read-out, associating the first set of representations with the target discrete entity, acquiring a second optical read-out of a marker of at least one discrete entity from the plurality of discrete entities, generating a second set of representations of the marker of the at least one discrete entity based on the second optical read-out, comparing the second set of representations to the first set of representations, and recognizing the at least one discrete entity as the target discrete entity upon determining that the second set of representations matches the first set of representations.

公开(公告)号：US20230410937A1

公开(公告)日：2023-12-21

申请号：US18332001

申请日：2023-06-09

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Soeren ALSHEIMER ,  Joachim BRADL ,  Kai WALTER

IPC: G16B5/20 ,  G01N21/64

CPC classification number: G16B5/20 ,  G01N21/6428 ,  G01N2021/6441

Abstract: A method for analysing a biological sample with analytes includes determining information about the biological sample and the analytes. The analytes are marked by a plurality of markers. The method further includes generating a probabilistic model of a distribution of the analytes within the biological sample based on the determined information, generating at least one optical readout of the biological sample, and determining presence of at least one analyte in the at least one optical readout based at least partially on the probabilistic model of the distribution of the analytes within the biological sample.

公开(公告)号：US20240410818A1

公开(公告)日：2024-12-12

申请号：US18698429

申请日：2022-09-30

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Soeren ALSHEIMER ,  Joachim BRADL

IPC: G01N21/05 ,  G01N21/64 ,  G02B21/00 ,  G02B21/33 ,  G02B21/34

Abstract: A sample carrier for receiving a sample includes an optical medium in which the sample is received, the optical medium having a first refractive index. A window portion defining two parallel surfaces includes an optically transparent material having a second refractive index, and is arranged at a bottom side of the sample carrier. The first and second refractive indices do not deviate by more than 2.5%.

公开(公告)号：US20240385196A1

公开(公告)日：2024-11-21

申请号：US18664312

申请日：2024-05-15

Applicant: Leica Microsystems CMS GmbH

Inventor： Soeren ALSHEIMER ,  Joachim BRADL

IPC: G01N33/58 ,  G01N21/64

Abstract: A method for assaying a plurality of biological samples is provided. The method includes preparing a plurality of discrete entities, each discrete entity comprising a polymeric compound, at least one biological sample, and a marker; arranging the plurality of discrete entities in an imaging container; generating at least one first optical read-out of at least one discrete entity; determining a first representation of the marker and at least one characteristic related to the at least one biological sample for the at least one discrete entity based on the at least one first optical read-out; and identifying the at least one discrete entity of the plurality of discrete entities from the imaging container as a discrete entity of interest based on the at least one characteristic related to the at least one biological sample and the first representation of the marker of the at least one discrete entity.

公开(公告)号：US20240255425A1

公开(公告)日：2024-08-01

申请号：US18290315

申请日：2021-12-23

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Soeren ALSHEIMER ,  Kai WALTER ,  Joachim BRADL

IPC: G01N21/64 ,  C12Q1/44

CPC classification number: G01N21/6428 ,  C12Q1/44 ,  G01N21/6458 ,  G01N2021/6419 ,  G01N2021/6441 ,  G01N2333/922

Abstract: A marker for marking a predetermined structure within a biological sample includes a marker base having an affinity reagent, and an attachment structure connected to the affinity reagent having two attachment sites. The attachment structure includes a cleavage site arranged between the attachment sites. The attachment structure is capable of being cut at the cleavage site by a cleaving agent in order to remove an attachment site from the marker base. The marker further includes at least two reporters. Each reporter includes a linker structure having a complementary attachment site configured to attach to one of the attachment sites, and a combination of at least two different fluorescent dyes. The combination of the at least two different fluorescent dyes is unique for each reporter. The complementary attachment site is unique for each reporter and configured such that each reporter attaches to a different attachment site of the marker base.

公开(公告)号：US20240071574A1

公开(公告)日：2024-02-29

申请号：US18452615

申请日：2023-08-21

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Joachim BRADL ,  Soeren ALSHEIMER

IPC: G16B50/30 ,  G06F16/22 ,  G06F16/2455

CPC classification number: G16B50/30 ,  G06F16/22 ,  G06F16/24564

Abstract: A data storage device includes an oligonucleotide nanostructure backbone with a plurality of attachment sites at predetermined positions, a plurality of labels configured to attach to the attachment sites, and at least a first orientation indicator and a second orientation indicator. Each label includes at least one dye, and an attachment oligonucleotide portion configured to attach to one of the attachment sites. The attachment oligonucleotide portion of each label includes a unique oligonucleotide sequence configured to bind to a complementary sequence of one of the attachment sites.