公开(公告)号：US10287342B2

公开(公告)日：2019-05-14

申请号：US15500921

申请日：2015-08-03

Applicant: Korea Advanced Institute of Science and Technology

Inventor： Hak-Sung Kim ,  Da Eun Hwang ,  Jung-Min Choi ,  Joong-Jae Lee ,  Woosung Heu

IPC: A61K39/00 ,  A61K39/02 ,  C07H21/04 ,  C07K16/18 ,  C12N15/62 ,  C07K14/725 ,  C07K14/34 ,  A61K38/00

Abstract: The present invention relates to novel polypeptide for binding to a complement protein C5a. More particularly, the present invention relates to polypeptide which can be bound to a complement protein C5a and inhibit the activation of same, polynucleotide which codes for the polypeptide, a recombinant vector which comprises the polynucleotide, a recombinant microorganism to which the recombinant vector has been introduced, a method for producing the polypeptide by means of the recombinant microorganism, and a pharmaceutical composition, for treating immune diseases or sepsis, containing the polypeptide. A polypeptide, according to the present invention, can be bound to a complement protein C5a, with higher affinity compared to being bound to a complement protein C5a receptor which is present in nature, and inhibits the activation of same, thus being widely utilized for development of formulation for preventing or treating diseases related to a complement protein C5a.

公开(公告)号：US20150133308A1

公开(公告)日：2015-05-14

申请号：US14538778

申请日：2014-11-11

Applicant: Korea Advanced Institute of Science and Technology ,  The Industry & Academic Cooperation in Chungnam National University ,  Korea Basic Science Institute

Inventor： Hak-Sung Kim ,  Joong-Jae Lee ,  Hyun-Ho Kyeong ,  Jung-Min Choi ,  Da-Eun Hwang ,  Hae-Kap Cheong ,  Hyun Jung Kim ,  Eun-Kyeong Jo ,  Chul-Su Yang ,  Jae-Min Yuk

IPC: C12N15/10 ,  C07K14/00

CPC classification number: C12N15/1058 ,  C07K14/195 ,  C07K14/461 ,  C07K2318/20 ,  C12N15/1037 ,  C40B40/10

Abstract: The present invention relates to a method for improving a repebody protein comprising repeat modules and a nucleotide library encoding a repebody protein library for improving the repebody protein. More particularly, the present invention relates to a method for improving a repebody protein using a module evolution method of sequentially mutating repeat modules constituting the repebody protein, and a nucleotide library encoding a repebody protein library used to improve the protein. According to the module evolution method of the present invention, an improved repebody protein can be screened which has a high binding affinity and accordingly increased specificity and activity, and thus it is easy to express a repebody used as an inhibitor, a therapeutic agent, and an analysis means against a target substance.

Abstract translation: 本发明涉及一种改进重复体蛋白质的方法，其包含重复单元和编码用于改善重组体蛋白的重组体蛋白文库的核苷酸文库。 更具体地说，本发明涉及使用序列突变构建重组体蛋白的重复模块的模块进化方法和编码用于改善蛋白质的重组体蛋白质文库的核苷酸文库来改进重组体蛋白质的方法。 根据本发明的模块进化方法，可以筛选出具有高结合亲和力并因此提高特异性和活性的改进的重组体蛋白，因此易于表达用作抑制剂，治疗剂和 分析意味着针对目标物质。