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公开(公告)号：US20030119204A1
公开(公告)日：2003-06-26
申请号：US10132673
申请日：2002-04-25
发明人： Ning Wei , Xuedong Song
IPC分类号： G01N033/558
CPC分类号： G01N33/54386 , G01N33/54393 , G01N33/558
摘要： A flow-through assay for detecting the quantity of an analyte residing in a test sample is provided. The flow-through assay contains a porous membrane that is in fluid communication with probe conjugates that contain a specific binding member and a detectable probe. The porous membrane also defines a detection zone and a calibration zone. The calibration zone includes two or more calibration regions (e.g., lines, dots, etc.) containing differing amounts of a binder that is configured to bind with the probe conjugates. As a result, calibration signals are generated that can be readily compared (visually, quantitatively, and the like) to a detection signal to determine the presence or quantity of an analyte in the test sample.
摘要翻译： 提供了用于检测驻留在测试样品中的分析物的量的流通测定。 流通测定包含多孔膜，其与含有特异性结合成员和可检测探针的探针缀合物流体连通。 多孔膜还限定了检测区域和校准区域。 校准区域包括两个或更多个校准区域（例如，线，点等），其包含不同量的配置成与探针共轭物结合的粘合剂。 结果，产生校准信号，其可以容易地比较（目视，定量等）到检测信号，以确定测试样品中分析物的存在或数量。
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公开(公告)号：US20030124739A1
公开(公告)日：2003-07-03
申请号：US10132421
申请日：2002-04-25
发明人： Xuedong Song , Ning Wei , Curt Sayre
IPC分类号： G01N033/558
CPC分类号： G01N33/54386 , G01N33/54393 , G01N33/558
摘要： A flow-through assay for detecting the quantity of an analyte residing in a test sample is provided. The flow-through assay contains a porous membrane that is in fluid communication with probe conjugates that contain a specific binding member and a detectable probe. The porous membrane also defines a detection zone and a calibration zone. The calibration zone contains a polyelectrolyte substantially non-diffusively immobilized on the porous membrane. The polyelectrolyte is capable of generating a detectable calibration signal that can be readily compared (visually, quantitatively, and the like) to a detection signal to determine the amount of analyte in the test sample.
摘要翻译： 提供了用于检测驻留在测试样品中的分析物的量的流通测定。 流通测定包含多孔膜，其与含有特异性结合成员和可检测探针的探针缀合物流体连通。 多孔膜还限定了检测区域和校准区域。 校准区域包含基本上非扩散地固定在多孔膜上的聚电解质。 聚电解质能够产生可检测的校准信号，其可以容易地与检测信号进行比较（视觉上，定量等）以确定测试样品中分析物的量。
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公开(公告)号：US20010040136A1
公开(公告)日：2001-11-15
申请号：US09746297
申请日：2000-12-19
发明人： Ning Wei , Bashir Musse Sheikh-Ali
IPC分类号： B01D037/00 , B01D039/14 , B05D003/00 , B05D003/10
CPC分类号： B01D39/2017 , B01D39/1607 , B01D39/2062 , B01D2239/0407 , B01D2239/0464 , B01D2239/0478 , B01D2239/0485 , B01D2239/0622 , B01D2239/0627 , B01D2239/0636 , B01D2239/083 , B01D2239/10 , C03C25/321 , C03C25/40 , C09D103/04
摘要： A glass or pretreated meltblown fiber having a cationically charged coating thereon, the coating including a functionalized cationically charged, silicon containing carbohydrate polymer crosslinkable by heat, in which the functionalized cationic polymer has been crosslinked by heat after being coated onto the glass fiber. Also provided is a fibrous filter including a fibrous filter media having a cationically charged coating thereon, the coating including a functionalized cationic polymer crosslinkable by heat, in which the functionalized cationic polymer has been crosslinked by heat after being coated onto the fibers. Further provided is a method of preparing a fibrous filter. The method involves providing a fibrous filter which includes glass fibers or pretreated nonwoven fibers, passing a solution of a functionalized cationic starch polymer crosslinkable by heat through a fibrous filter under conditions sufficient to substantially coat the fibers with the functionalized cationic polymer, and treating the resulting coated fibrous filter with heat at a temperature and for a time sufficient to crosslink the functionalized cationic polymer present on the glass fibers.
摘要翻译： 一种玻璃或预处理的熔喷纤维，其上具有阳离子电荷的涂层，该涂层包括通过加热可交联的功能化阳离子电荷的含硅碳水化合物聚合物，其中官能化阳离子聚合物在被涂覆到玻璃纤维上之后通过热交联。 还提供了一种纤维过滤器，其包括其上具有阳离子电荷的涂层的纤维过滤介质，该涂层包括通过加热可交联的官能化阳离子聚合物，其中官能化阳离子聚合物在被涂覆在纤维上之后已被热交联。 进一步提供一种制备纤维过滤器的方法。 该方法包括提供纤维过滤器，其包括玻璃纤维或经预处理的非织造纤维，使官能化的阳离子淀粉聚合物的溶液通过热可交联的纤维过滤器在足以基本上用官能化阳离子聚合物涂覆纤维的条件下进行处理， 在足以使存在于玻璃纤维上的官能化阳离子聚合物交联的温度和时间下加热涂布纤维过滤器。
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公开(公告)号：US20040121480A1
公开(公告)日：2004-06-24
申请号：US10325614
申请日：2002-12-19
发明人： Ning Wei , Yanbin Huang
IPC分类号： G01N030/02 , G01N031/22
CPC分类号： G01N33/558 , G01N33/538 , G01N33/5432 , Y10S436/805 , Y10S436/809
摘要： A membrane-based assay device for detecting the presence or quantity of an analyte residing in a test sample is provided. The device utilizes a chromatographic zone on which is disposed a plurality of microporous particles. The chromatographic zone can effectively reduce the nullhook effectnull in a simple, efficient, and relatively inexpensive manner. In particular, the plurality of microporous particles allows larger-sized analyte/probe complexes to reach the detection zone before the uncomplexed analyte. Because the uncomplexed analyte is substantially inhibited from competing with the complexes for the binding sites at the detection zone, the incidence of nullfalse negativesnull may be limited, even at relatively high analyte concentrations.
摘要翻译： 提供了一种用于检测驻留在测试样品中的分析物的存在或数量的基于膜的测定装置。 该装置利用其上设置有多个微孔颗粒的色谱区。 色谱区域可以以简单，高效，相对便宜的方式有效降低“钩效应”。 特别地，多个微孔颗粒允许更大尺寸的分析物/探针复合物在未复合分析物之前到达检测区。 由于未配合的分析物被基本上抑制在与检测区域的结合位点的配合物竞争中，所以即使在相对高的分析物浓度下，“假阴性”的发生率可能受到限制。
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5.发明申请Cationically charged coating on hydrophobic polymer fibers with poly (vinyl alcohol) assist 有权疏水性聚合物纤维与聚（乙烯醇）辅助的阳离子带电涂层公开(公告)号：US20020122939A1
公开(公告)日：2002-09-05
申请号：US10053068
申请日：2002-01-18
IPC分类号： B32B027/00
CPC分类号： D06M15/59 , B01D39/1623 , B01D2239/0428 , B01D2239/0492 , D06M15/333 , D06M15/55 , D06M15/61 , D06M23/10 , Y10T428/249921 , Y10T428/29 , Y10T428/2929 , Y10T428/2933 , Y10T428/2967
摘要： A hydrophobic polymer fiber having a cationically charged coating thereon, in which the coating includes a functionalized cationic polymer, such as an epichlorohydrin-functionalized polyamine or an epichlorohydrin-functionalized polyamido-amine, which has been crosslinked by heat. The present invention also provides a fibrous filter which includes hydrophobic polymer fibers having the foregoing cationically charged coating thereon. The present invention further provides a method of preparing a fibrous filter. The method involves providing a fibrous filter comprised of hydrophobic polymer fibers; treating the fibrous filter with an aqueous solution of a functionalized cationic polymer crosslinkable by heat under conditions sufficient to substantially coat the fibers with the functionalized cationic polymer, wherein the solution includes the functionalized cationic polymer, a poly(vinyl alcohol), a polar solvent for the poly(vinyl alcohol), and water; and treating the resulting coated fibrous filter with heat at a temperature and for a time sufficient to crosslink the functionalized cationic polymer present on the hydrophobic polymer fibers. The present invention additionally provides a method of preparing a fibrous filter. The method includes providing a fibrous filter comprised of hydrophobic polymer fibers; passing a solution of a functionalized cationic polymer crosslinkable by heat through the fibrous filter under conditions sufficient to substantially coat the fibers with the functionalized cationic polymer, wherein the solution includes the functionalized cationic polymer, a poly(vinyl alcohol), and water; and treating the resulting coated fibrous filter with heat at a temperature and for a time sufficient to crosslink the functionalized cationic polymer present on the hydrophobic polymer fibers.
摘要翻译： 一种其上具有阳离子电荷涂层的疏水性聚合物纤维，其中涂层包括官能化的阳离子聚合物，例如经过热交联的表氯醇官能化的多胺或表氯醇官能化的聚酰胺胺。 本发明还提供一种纤维过滤器，其包括其上具有上述阳离子电荷涂层的疏水性聚合物纤维。 本发明还提供一种制备纤维过滤器的方法。 该方法包括提供由疏水性聚合物纤维组成的纤维过滤器; 在足以使所述官能化阳离子聚合物基本上涂覆所述纤维的条件下通过热可交联的官能化阳离子聚合物的水溶液处理所述纤维过滤器，其中所述溶液包括所述官能化阳离子聚合物，聚（乙烯醇），极性溶剂 聚（乙烯醇）和水; 并在足以使存在于疏水性聚合物纤维上的官能化阳离子聚合物交联的温度和时间下用热处理所得到的涂覆的纤维过滤器。 本发明另外提供了制备纤维过滤器的方法。 该方法包括提供由疏水聚合物纤维组成的纤维过滤器; 使官能化阳离子聚合物的溶液通过纤维过滤器在足以基本上用官能化阳离子聚合物涂覆纤维的条件下进行交联，其中该溶液包括官能化阳离子聚合物，聚（乙烯醇）和水; 并在足以使存在于疏水性聚合物纤维上的官能化阳离子聚合物交联的温度和时间下用热处理所得到的涂覆的纤维过滤器。
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公开(公告)号：US20040197820A1
公开(公告)日：2004-10-07
申请号：US10406631
申请日：2003-04-03
发明人： Ning Wei , Yanbin Huang , Kaiyuan Yang
IPC分类号： G01N033/53 , G01N033/558
CPC分类号： G01N33/587 , G01N33/54393 , G01N33/558
摘要： A membrane-based assay device for detecting the presence or quantity of an analyte residing in a test sample is provided. The device utilizes conjugated probes that contain a specific binding member for the analyte of interest. The specific binding member preferentially complexes with the analyte within a test sample when contacted therewith. Excess analyte that remains uncomplexed with the specific binding member undergoes non-specific binding, such as to a hydrophobic domain. As a result, the ability of the uncomplexed analyte to compete with the complexed analyte at the detection zone of the device is restricted. Thus, the incidence of nullfalse negativesnull is limited in a simple, efficient, and relatively inexpensive manner.
摘要翻译： 提供了一种用于检测驻留在测试样品中的分析物的存在或数量的基于膜的测定装置。 该装置使用包含感兴趣分析物的特异性结合成员的缀合探针。 当与其接触时，特异性结合成分优先与测试样品内的分析物复合。 与特异性结合成员保持未复合的过量分析物经历非特异性结合，例如疏水结构域。 因此，未配合的分析物在装置的检测区域与复合分析物竞争的能力受到限制。 因此，“假阴性”的发生率以简单，有效和相对便宜的方式受到限制。
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公开(公告)号：US20040197819A1
公开(公告)日：2004-10-07
申请号：US10406577
申请日：2003-04-03
发明人： Kaiyuan Yang , Ning Wei , Rosann Kaylor , Chibueze Obinna Chidebelu-Eze , Zdravko Savov Atanassov
IPC分类号： G01N033/53 , C12M003/00
CPC分类号： G01N33/587 , G01N33/558
摘要： Hollow particles for use in various types of assay devices are provided. Due to their hollow or voided structure, the particles may exhibit a variety of beneficial properties. For instance, hollow particles are generally lightweight, and thus, relatively inexpensive in comparison to other types of particles. Hollow particles may also form a stable system without requiring refrigeration or rotation. In addition, hollow particles may possess enhanced light diffraction capabilities, which may be particularly beneficial in certain types of assay devices, e.g., diffraction-based assay devices.
摘要翻译： 提供了用于各种类型的测定装置的中空颗粒。 由于其中空或空隙结构，颗粒可能表现出各种有益的性质。 例如，中空颗粒通常是轻质的，因此与其它类型的颗粒相比相对便宜。 中空颗粒也可以形成稳定的系统，而不需要制冷或旋转。 此外，中空颗粒可以具有增强的光衍射能力，这在某些类型的测定装置中是特别有益的，例如基于衍射的测定装置。
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公开(公告)号：US20040121334A1
公开(公告)日：2004-06-24
申请号：US10325429
申请日：2002-12-19
发明人： Ning Wei , Yanbin Huang , Xuedong Song , Rosann Kaylor
IPC分类号： C12Q001/68 , C12M001/34
CPC分类号： G01N33/558 , G01N33/54393
摘要： An internal, self-calibrated system for flow-through assay devices is provided. In particular, the present invention employs the use of a single calibration/detection zone defined by a porous membrane of the assay. It has been discovered that the internal, self-calibrated system provides an accurate, inexpensive, and readily controllable method of determining the presence of an analyte in a test sample.
摘要翻译： 提供了一种用于流通测定装置的内部自校准系统。 特别地，本发明使用由测定的多孔膜限定的单个校准/检测区域。 已经发现，内部的自校准系统提供了确定测试样品中分析物的存在的精确，便宜且易于控制的方法。
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公开(公告)号：US20040043502A1
公开(公告)日：2004-03-04
申请号：US10286342
申请日：2002-11-01
发明人： Xuedong Song , Rosann Kaylor , Michael Knotts , Ning Wei
IPC分类号： G01N021/64
CPC分类号： G01N33/54326 , G01N21/274 , G01N21/6408 , G01N33/525 , G01N33/558 , G01N33/582 , G01N2021/6439 , Y10S435/803 , Y10S435/805 , Y10S435/97 , Y10S436/80 , Y10S436/806 , Y10S436/824 , Y10T436/13
摘要： A membrane-based assay device for detecting the presence or quantity of an analyte residing in a test sample is provided. The device utilizes time-resolved fluorescence to detect the signals generated by excited fluorescent labels. Because the labels can have relatively long emission lifetime, short-lived background interference can be practically eliminated through delayed fluorescence detection. In addition, the resulting fluorescent reader can have a simple and inexpensive design. For instance, in one embodiment, the reader can utilize a silicon photodiode and a pulsed light-emitting diode (LED) to accurately excite labels and detect fluorescence on a membrane-based assay device without requiring the use of expensive components, such as monochromators or narrow emission band width optical filters.
摘要翻译： 提供了一种用于检测驻留在测试样品中的分析物的存在或数量的基于膜的测定装置。 该装置利用时间分辨荧光检测激发的荧光标记产生的信号。 因为标签的发光寿命可以较长，所以通过延迟荧光检测实际上可以消除短暂的背景干扰。 此外，所得到的荧光读取器可以具有简单且便宜的设计。 例如，在一个实施例中，读取器可以利用硅光电二极管和脉冲发光二极管（LED）来精确地激发标签并在基于膜的测定装置上检测荧光，而不需要使用昂贵的组件，例如单色仪或 窄发射带宽滤光片。
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公开(公告)号：US20030119203A1
公开(公告)日：2003-06-26
申请号：US10035014
申请日：2001-12-24
发明人： Ning Wei , Xuedong Song
IPC分类号： G01N033/558
CPC分类号： G01N33/54386 , G01N33/54393 , G01N33/558
摘要： Disclosed is a method and apparatus for employing, in a lateral flow assay, multiple control lines to assist in improving the sensitivity of such an assay. Analytes of interest may be quantified in a lateral flow assay by conducting internally derived calibrations by quantifying the analyte and calibrating the assay device, at essentially the same time, on the same device. That is, calibration and sample testing may occur simultaneously, improving sensitivity, and reducing errors that otherwise may be introduced by comparing data produced in one assay with data or reference data produced in a different assay. A multi-point calibration technique may be employed. Visual spectrophotographic reading devices may be employed to compare intensity of signals generated by probes attached to the analyte with probes associated with control lines upon a calibration zone.
摘要翻译： 公开了一种在侧流测定中采用多个对照品系以帮助提高这种测定的灵敏度的方法和装置。 感兴趣的分析物可以通过在同一装置上基本上同时定量分析物和校准测定装置进行内部衍生的校准来在侧流测定中量化。 也就是说，校准和样本测试可以同时发生，提高灵敏度，并减少否则可能通过将在一个测定中产生的数据与在不同测定中产生的数据或参考数据进行比较而引入的错误。 可以采用多点校准技术。 可以使用视觉分光光度读取装置来比较在分析物上连接的探针产生的信号强度与在校准区域上与控制线相关联的探针。
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