公开(公告)号：US5215897A

公开(公告)日：1993-06-01

申请号：US525302

申请日：1990-05-17

Applicant: Keiichi Sakashita ,  Tetsuji Nakamura ,  Ichiro Watanabe

Inventor： Keiichi Sakashita ,  Tetsuji Nakamura ,  Ichiro Watanabe

IPC: C12N9/80 ,  C12P13/04 ,  C12P41/00

CPC classification number: C12N9/80 ,  C12P13/04 ,  C12P41/006 ,  Y10S435/828 ,  Y10S435/874

Abstract: A process for producing an L-amino acid from the corresponding DL- and/or L-amino acid amide represented by the general formula: ##STR1## wherein R is a substituted or unsubstituted alkyl group having one to 4 carbon atoms, a substituted or unsubstituted phenyl group, or a substituted or unsubstituted aralkyl group, by action of an enzyme having hydrolytic activity to L-amino acid amides which is produced by Enterobacter cloacae or Pseudomonas sp.

Abstract translation: 由下列通式表示的相应的DL-和/或L-氨基酸酰胺生产L-氨基酸的方法：其中R是取代或未取代的具有1至4个碳原子的烷基，取代或 未取代的苯基或取代或未取代的芳烷基，通过对由阴沟肠杆菌或假单胞菌属（Pseudomonas sp。）产生的L-氨基酸酰胺具有水解活性的酶的作用。

公开(公告)号：US5166061A

公开(公告)日：1992-11-24

申请号：US555001

申请日：1990-07-20

Applicant: Tetsuji Nakamura ,  Fujio Yu ,  Ichiro Watanabe

Inventor： Tetsuji Nakamura ,  Fujio Yu ,  Ichiro Watanabe

IPC: C12N1/20 ,  C12P13/00 ,  C12R1/15 ,  C12R1/32

CPC classification number: C12P13/002 ,  Y10S435/822 ,  Y10S435/843

Abstract: A process for the production of a 4-halo-3-hydroxybutyronitrile which comprises reacting a 1,3-dihalo-2-propanol with a dehalogenating enzyme originating from a Corynebacterium or a Microbacterium in the presence of an alkali cyanide to thereby convert the 1,3-dihalo-2-propanol into the 4-halo-3-hydroyxbutyronitrile and collecting the product thus formed is disclosed. According to this process, a 4-halo-3-hydroxybutyronitrile which is highly useful in the syntheses of various medicines and physiologically active substances can be easily and efficiently produced from an inexpensive starting material.

Abstract translation: 一种4-卤代-3-羟基丁腈的制备方法，其包括在碱金属氰化物存在下使1,3-二卤代-2-丙醇与源自棒杆菌或微杆菌的脱卤素酶反应，从而将1 ，3-二卤代-2-丙醇加入到4-卤代-3-羟基丁腈中并收集如此形成的产品。 根据该方法，可以从廉价的原料容易且有效地制备在各种药物和生理活性物质的合成中非常有用的4-卤代-3-羟基丁腈。

公开(公告)号：US5155030A

公开(公告)日：1992-10-13

申请号：US511377

申请日：1990-04-19

Applicant: Tetsuji Nakamura ,  Ichiro Watanabe

Inventor： Tetsuji Nakamura ,  Ichiro Watanabe

IPC: C12P7/18 ,  C12P41/00 ,  C12R1/01 ,  C12R1/15

CPC classification number: C12P7/18 ,  C12P41/00 ,  Y10S435/843

Abstract: A process for preparing an optically active (R)-(-)-3-halo-1,2-propanediol comprising reacting an epihalohydrin with an epihalohydrin hydratase originating from a microorganism. For example, the microorganism belongs to the genus Corynebacterium or the genus Microbacterium. Epihalohydrins which can be used include epichlorohydrin and epibromohydrin. The reaction can be carried out at a temperature of from 5.degree. to 50.degree. C. and a pH of from 4 to 10.

Abstract translation: 一种制备光学活性（R） - （ - ） - 3-卤代-1,2-丙二醇的方法，包括使表卤代醇与源自微生物的表卤代醇水合酶反应。 例如，微生物属于棒杆菌属或微杆菌属。 可以使用的包括表氯醇和表溴醇。 该反应可以在5℃至50℃的温度和4至10℃的pH下进行。

公开(公告)号：US4943528A

公开(公告)日：1990-07-24

申请号：US438124

申请日：1989-11-20

Applicant: Tetsuji Nakamura ,  Ichiro Watanabe

Inventor： Tetsuji Nakamura ,  Ichiro Watanabe

IPC: C12P7/18 ,  C12P41/00

CPC classification number: C12P41/00 ,  C12P7/18

Abstract: A process for the production of an optically active (R)-(-)-3-halo-1,2-propanediol which comprises contacting a 1,3-dihalo-2-propanol with a dehalogenase originating from a microorganism belonging to the genus Corynebacterium or Microbacterium. This process makes it possible to produce the (R)-(-)-3-halo-1,2-propanediol, which is highly useful as a starting material in the synthesis of various drugs and physiologically active substances, theoretically at a yield of 100%.

Abstract translation: 一种制备光学活性（R） - （ - ） - 3-卤代-1,2-丙二醇的方法，该方法包括使1,3-二卤-2-丙醇与源自属于该属的微生物的脱卤素酶接触 棒状杆菌或微杆菌。 该方法使得可以生产在各种药物和生理活性物质的合成中作为原料高度有用的（R） - （ - ） - 3-卤代-1,2-丙二醇，理论上以产率 100％。

公开(公告)号：US07803578B2

公开(公告)日：2010-09-28

申请号：US11947955

申请日：2007-11-30

Applicant: Fumiaki Watanabe ,  Dai Ujihara ,  Miki Sakai ,  Fujio Yu ,  Tetsuji Nakamura

Inventor： Fumiaki Watanabe ,  Dai Ujihara ,  Miki Sakai ,  Fujio Yu ,  Tetsuji Nakamura

IPC: C12P21/06 ,  C12N9/00 ,  C12N9/88 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04

CPC classification number: C12P13/02 ,  C12N9/88

Abstract: The present invention provides: a protein having an improved nitrile hydratase activity, whereby heat resistance has been improved when compared with a wild-type nitrile hydratase activity, wherein the amino acid sequence of a nitrile hydratase is modified; a gene DNA encoding the above protein; a recombinant vector having the above gene DNA; a transformant or transductant having the above recombinant vector; a nitrile hydratase collected from a culture of the above transformant or transductant, and a production method thereof; and a method for producing an amide compound.

Abstract translation: 本发明提供：腈水合酶活性提高的蛋白质，与野生型腈水合酶活性相比，耐水性得到改善，其中腈水合酶的氨基酸序列被修饰; 编码上述蛋白质的基因DNA; 具有上述基因DNA的重组载体; 具有上述重组载体的转化体或转导体; 从上述转化体或转导体的培养物收集的腈水合酶及其制备方法; 和酰胺化合物的制造方法。

公开(公告)号：US20090162894A1

公开(公告)日：2009-06-25

申请号：US11947955

申请日：2007-11-30

Applicant: Fumiaki Watanabe ,  Dai Ujihara ,  Miki Sakai ,  Fujio Yu ,  Tetsuji Nakamura

Inventor： Fumiaki Watanabe ,  Dai Ujihara ,  Miki Sakai ,  Fujio Yu ,  Tetsuji Nakamura

IPC: C07H21/04 ,  C12N15/63 ,  C12N1/21 ,  C12P21/00

CPC classification number: C12P13/02 ,  C12N9/88

Abstract: The present invention provides: a protein having an improved nitrile hydratase activity, whereby heat resistance has been improved when compared with a wild-type nitrile hydratase activity, wherein the amino acid sequence of a nitrile hydratase is modified; a gene DNA encoding the above protein; a recombinant vector having the above gene DNA; a transformant or transductant having the above recombinant vector; a nitrile hydratase collected from a culture of the above transformant or transductant, and a production method thereof; and a method for producing an amide compound.

Abstract translation: 本发明提供：腈水合酶活性提高的蛋白质，与野生型腈水合酶活性相比，耐水性得到改善，其中腈水合酶的氨基酸序列被修饰; 编码上述蛋白质的基因DNA; 具有上述基因DNA的重组载体; 具有上述重组载体的转化体或转导体; 从上述转化体或转导体的培养物收集的腈水合酶及其制备方法; 和酰胺化合物的制造方法。

公开(公告)号：US07098889B2

公开(公告)日：2006-08-29

申请号：US10606701

申请日：2003-06-26

Applicant: Takashi Inui ,  Tetsuji Nakamura

Inventor： Takashi Inui ,  Tetsuji Nakamura

IPC: H03G3/02 ,  G09G5/00

CPC classification number: G06F3/038

Abstract: A device, method, and computer program product for a self-calibrating input device are provided. The input device includes a designated value change unit operable to change a designated value using a variation amount associated with the designated value, the designated value being changed using a predetermined first variation amount when a first designated value change directive is received from an operator, and a variation amount change unit operable to alter the variation amount associated with the designated value from the predetermined first variation amount to a smaller second variation amount when a second designated value change directive that is opposite the first designated value change directive is received from the operator within a predetermined variation amount change period.

Abstract translation: 提供了一种用于自校准输入设备的设备，方法和计算机程序产品。 所述输入装置包括指定值改变单元，其可操作以使用与所述指定值相关联的变化量来改变指定值，当从操作者接收到第一指定值改变指令时，所述指定值使用预定的第一变化量进行变化;以及 变更量变更单元，用于当从所述操作者接收到与所述第一指定值变更指令相反的第二指定值变更指令时，将与所述指定值相关联的所述变更量从所述预定第一变更量变更为较小的第二变更量 预定变化量变化期间。

公开(公告)号：US08728792B2

公开(公告)日：2014-05-20

申请号：US12691443

申请日：2010-01-21

Applicant: Takanori Akiyama ,  Wataru Mizunashi ,  Tetsuji Nakamura

Inventor： Takanori Akiyama ,  Wataru Mizunashi ,  Tetsuji Nakamura

IPC: C12N9/88 ,  C12P13/00 ,  C07H21/04

CPC classification number: C12N9/88 ,  C12P13/04

Abstract: The present invention provides a modified ethylenediamine-N,N′-disuccinate:ethylenediamine lyase. The present invention also provides a protein that comprises the amino acid sequence represented by SEQ ID NO: 1; or a protein that comprises an amino acid sequence derived from the amino acid sequence represented by SEQ ID NO: 1 by deletion, substitution, or addition of one or more amino acid residues, and has an ethylenediamine-N,N′-disuccinate:ethylenediamine lyase activity.

Abstract translation: 本发明提供了改性的乙二胺-N，N'-二琥珀酸盐：乙二胺裂解酶。 本发明还提供了包含SEQ ID NO：1所示的氨基酸序列的蛋白质; 或包含通过缺失，取代或添加一个或多个氨基酸残基而衍生自SEQ ID NO：1所示氨基酸序列的氨基酸序列的蛋白质，并且具有乙二胺-N，N'-二琥珀酸盐：乙二胺 裂解酶活性。

公开(公告)号：US07645605B2

公开(公告)日：2010-01-12

申请号：US11569529

申请日：2005-05-26

Applicant: Fumiaki Watanabe ,  Dai Ujihara ,  Miki Sakai ,  Fujio Yu ,  Tetsuji Nakamura

Inventor： Fumiaki Watanabe ,  Dai Ujihara ,  Miki Sakai ,  Fujio Yu ,  Tetsuji Nakamura

IPC: C12N9/88 ,  C12N9/00 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04

CPC classification number: C12P13/02 ,  C12N9/88

Abstract: The present invention provides: a protein having an improved nitrile hydratase activity, whereby heat resistance has been improved when compared with a wild-type nitrile hydratase activity, wherein the amino acid sequence of a nitrile hydratase is modified; a gene DNA encoding the above protein; a recombinant vector having the above gene DNA; a transformant or transductant having the above recombinant vector; a nitrile hydratase collected from a culture of the above transformant or transductant, and a production method thereof; and a method for producing an amide compound.

公开(公告)号：US07579485B2

公开(公告)日：2009-08-25

申请号：US10502595

申请日：2003-02-03

Applicant: Makoto Kaneko ,  Yasuhiro Ninomiya ,  Tetsuji Nakamura ,  Eiji Satou

Inventor： Makoto Kaneko ,  Yasuhiro Ninomiya ,  Tetsuji Nakamura ,  Eiji Satou

IPC: C07D307/02

CPC classification number: C07D307/33 ,  C07D309/30 ,  C07D315/00 ,  C12P13/02 ,  C12P17/04 ,  C12P17/06

Abstract: A method for producing lactones, which comprises reacting an amide compound of Formula (I): [wherein X represents a halogen atom; R, R′ and R1 to R6 each independently represents a hydrogen atom or any desired substituent; and n represents an integer of 0 to 2] with an aqueous medium.

Abstract translation: 一种制备内酯的方法，包括使式（I）的酰胺化合物：其中X表示卤素原子; R，R'和R 1至R 6各自独立地表示氢原子或任何所需的取代基; 和n表示0〜2的整数]。