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    GENETICALLY MODIFIED FUNGI AND THEIR USE IN LIPID PRODUCTION
    4.
    发明申请
    GENETICALLY MODIFIED FUNGI AND THEIR USE IN LIPID PRODUCTION 有权
    遗传改良真菌及其在脂生产中的应用

    公开(公告)号：US20130102042A1

    公开(公告)日：2013-04-25

    申请号：US13806281

    申请日：2011-06-21

    申请人： Kari Koivuranta ,  Laura Ruohonen ,  Merja Penttilä

    发明人： Kari Koivuranta ,  Laura Ruohonen ,  Merja Penttilä

    IPC分类号： C12P7/64 ,  C12N15/80

    CPC分类号： C12N15/52 ,  C12N9/0008 ,  C12N9/1029 ,  C12N9/88 ,  C12N9/93 ,  C12N15/80 ,  C12P7/6463 ,  C12P7/649 ,  C12Y102/0101 ,  C12Y203/0102 ,  C12Y401/01001 ,  C12Y602/01001 ,  Y02E50/13 ,  Y02P20/52

    摘要： The invention refers to fungal cells, and especially to oleaginous fungal cells that have been genetically modified to produce enzymes of the pyruvate dehydrogenase bypass route to enhance their lipid production. Especially the cells are modified to over-express genes encoding pyruvate decarboxylase (PDC), acetaldehyde dehydrogenase (ALD) and/or acetyl-CoA synthetase (ACS), optionally together with a gene encoding diacylglycerol acyltransferase (DAT), or to express genes encoding PDC together with ALD and/or ACS. Methods of producing lipids, biofuels and lubricants using the modified fungi are also disclosed as well as expression cassettes useful therein. A new enzyme having phosholipid: diacylglycerol acyltransferase (PDAT)activity it and a polynucleotide encoding it are also disclosed, which are useful in the lipid production. A recombinant Cryptococcus cell and its construction is described.

    摘要翻译： 本发明涉及真菌细胞，尤其涉及已被遗传修饰以产生丙酮酸脱氢酶旁路途径的酶以增强其脂质产生的油质真菌细胞。 特别地，细胞被修饰为过度表达编码丙酮酸脱羧酶（PDC），乙醛脱氢酶（ALD）和/或乙酰辅酶A合成酶（ACS）的基因，任选与编码二酰基甘油酰基转移酶（DAT）的基因一起，或表达编码 PDC与ALD和/或ACS。 还公开了使用改性真菌生产脂质，生物燃料和润滑剂的方法以及在其中有用的表达盒。 还公开了具有磷脂的新型酶：二酰基甘油酰基转移酶（PDAT）活性及其编码它的多核苷酸，其可用于脂质生产。 描述了重组隐球藻细胞及其构建。

    Genetically modified fungi and their use in lipid production
    7.
    发明授权
    Genetically modified fungi and their use in lipid production 有权
    转基因真菌及其在脂质生产中的应用

    公开(公告)号：US09518276B2

    公开(公告)日：2016-12-13

    申请号：US13806281

    申请日：2011-06-21

    申请人： Kari Koivuranta ,  Laura Ruohonen ,  Merja Penttilä

    发明人： Kari Koivuranta ,  Laura Ruohonen ,  Merja Penttilä

    IPC分类号： C12P7/04 ,  C12P7/64 ,  C12N9/02 ,  C12N9/10 ,  C12N9/88 ,  C12N9/00 ,  C12N15/80

    CPC分类号： C12N15/52 ,  C12N9/0008 ,  C12N9/1029 ,  C12N9/88 ,  C12N9/93 ,  C12N15/80 ,  C12P7/6463 ,  C12P7/649 ,  C12Y102/0101 ,  C12Y203/0102 ,  C12Y401/01001 ,  C12Y602/01001 ,  Y02E50/13 ,  Y02P20/52

    摘要： The invention refers to fungal cells, and especially to oleaginous fungal cells that have been genetically modified to produce enzymes of the pyruvate dehydrogenase bypass route to enhance their lipid production. Especially the cells are modified to over-express genes encoding pyruvate decarboxylase (PDC), acetaldehyde dehydrogenase (ALD) and/or acetyl-CoA synthetase (ACS), optionally together with a gene encoding diacylglycerol acyltransferase (DAT), or to express genes encoding PDC together with ALD and/or ACS. Methods of producing lipids, biofuels and lubricants using the modified fungi are also disclosed as well as expression cassettes useful therein. A new enzyme having phospholipid: diacylglycerol acyltransferase (PDAT) activity and a polynucleotide encoding it are also disclosed, which are useful in the lipid production. A recombinant Cryptococcus cell and its construction is described.

    摘要翻译： 本发明涉及真菌细胞，尤其涉及已被遗传修饰以产生丙酮酸脱氢酶旁路途径的酶以增强其脂质产生的油质真菌细胞。 特别地，细胞被修饰为过度表达编码丙酮酸脱羧酶（PDC），乙醛脱氢酶（ALD）和/或乙酰辅酶A合成酶（ACS）的基因，任选与编码二酰基甘油酰基转移酶（DAT）的基因一起，或表达编码 PDC与ALD和/或ACS。 还公开了使用改性真菌生产脂质，生物燃料和润滑剂的方法以及在其中有用的表达盒。 还公开了具有磷脂：二酰基甘油酰基转移酶（PDAT）活性的新酶和编码它的多核苷酸，其可用于脂质生产。 描述了重组隐球藻细胞及其构建。