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    High efficacy antisense RIαPKA poly-DNP oligoribonucleotides
    1.
    发明授权
    High efficacy antisense RIαPKA poly-DNP oligoribonucleotides 失效
    高效反义RIalphaPKA多聚DNP寡核糖核苷酸

    公开(公告)号：US07528117B2

    公开(公告)日：2009-05-05

    申请号：US10728491

    申请日：2003-12-05

    申请人： Jui H. Wang ,  Long Shen ,  Xiaolan Chen

    发明人： Jui H. Wang ,  Long Shen ,  Xiaolan Chen

    IPC分类号： A01N43/04 ,  C12Q1/68 ,  C12P19/34 ,  C07H21/02 ,  C07H21/04

    CPC分类号： C12N15/113 ,  A61K38/00 ,  C12N15/1137 ,  C12N2310/321 ,  C12N2310/3529

    摘要： The present invention discloses antisense poly-2′-O-(2,4-dinitrophenyl) oligoribonucleotides which are capable of down regulating the expression of the RIα subunit of protein kinase A. An example is 5′-GGCUGCGUGCCUCCUCACUGG (named antisense poly-DNP RNA-21) or a sequence which has a one-base mismatch therewith. The antisense oligoribonucleotide can be synthesized by in vitro transcription followed by chemical derivatization. The base sequence of the oligoribonucleotides is complementary to that of nt 110 to 130 in RIα/PKA mRNA. The antisense poly-DNP RNA-21 was found to inhibit cell growth with IC50 values in the nanomolar range. These oligonucleotides can be used as effective anti-cancer agents.

    摘要翻译： 本发明公开了能够下调蛋白激酶A的RIalpha亚基表达的反式多聚-2'-O-（2,4-二硝基苯基）寡核糖核苷酸。一个实例是5'-GGCUGCGUGCCUCCUCACUGG（命名为反义多聚DNP RNA-21）或与其单碱基错配的序列。 反义寡核苷酸可以通过体外转录，然后进行化学衍生化合成。 寡核糖核苷酸的碱基序列与RIalpha / PKA mRNA中的nt 110至130的碱基序列互补。 发现反义多聚DNP RNA-21抑制细胞生长，其IC50值在纳摩尔范围内。 这些寡核苷酸可以用作有效的抗癌剂。

    In Vitro and in vivo silencing of human c-myc oncogene expression by poly-DNP-RNA
    2.
    发明申请
    In Vitro and in vivo silencing of human c-myc oncogene expression by poly-DNP-RNA 审中-公开
    体外和体内沉默人类c-myc癌基因表达多聚DNP-RNA

    公开(公告)号：US20060122140A1

    公开(公告)日：2006-06-08

    申请号：US11284693

    申请日：2005-11-22

    申请人： Jui Wang ,  Long Shen ,  Chongjie Zhang ,  Julian Ambrus

    发明人： Jui Wang ,  Long Shen ,  Chongjie Zhang ,  Julian Ambrus

    IPC分类号： A61K48/00

    CPC分类号： C12N15/1135 ,  A61K2121/00 ,  C12N2310/11 ,  C12N2310/321 ,  C12N2310/3529

    摘要： The present invention provides antisense oligoribonucleotides with sequences that recognize and bind to the c-myc gene or c-myc mRNA, where one or more sugar residues of the oligoribonucleotides are modified by the substitution of DNP at the 2′-O position. The antisense oligoribonucleotides can be used for inhibiting the growth of cells in which the c-myc gene is overexpressed by down-regulation of transcription of c-myc RNA or translation of the c-myc protein. The antisense oligoribonucleotides can also be used for diagnostic purposes to identify the overexpression of the c-myc gene. The nucleotide sequence of the DNP-oligoribonucleotides is complementary to the sequence 578 to 598 in c-myc mRNA and does not contain a G-quartet motif. This DNP-RNA can silence c-myc gene expression both in vitro and in vivo with high efficacy and sequence specificity.

    摘要翻译： 本发明提供了具有识别和结合c-myc基因或c-myc mRNA的序列的反义寡核苷酸，其中寡核糖核苷酸的一个或多个糖残基通过在2'-O位置处的DNP的取代被修饰。 反义寡核糖核苷酸可用于抑制c-myc基因通过下调c-myc RNA的转录或c-myc蛋白的翻译而过表达的细胞的生长。 反义寡核糖核酸也可用于诊断目的，以鉴定c-myc基因的过表达。 DNP-寡核糖核苷酸的核苷酸序列与c-myc mRNA中的序列578至598互补，并且不含G-四重基序列。 该DNP-RNA可以在体外和体内沉默c-myc基因表达，具有高效和序列特异性。

    Method of Diagnosing Sjogren's Disease
    4.
    发明申请
    Method of Diagnosing Sjogren's Disease 有权
    诊断干燥病的方法

    公开(公告)号：US20130040322A1

    公开(公告)日：2013-02-14

    申请号：US13574265

    申请日：2011-01-21

    申请人： Julian L. Ambrus ,  Long Shen

    发明人： Julian L. Ambrus ,  Long Shen

    IPC分类号： G01N33/566 ,  C07K14/47 ,  C07K17/00 ,  C12N9/88

    CPC分类号： G01N33/6893 ,  G01N33/564 ,  G01N2333/47 ,  G01N2333/988 ,  G01N2800/04 ,  G01N2800/101

    摘要： Provided are methods and compositions for determining whether an individual has Sjögren's disease (SD). The method entails determining in a biological sample from the individual the presence of antibodies directed to salivary gland protein 1 (SP-I), parotid secretory protein (PSP), carbonic anhydrase 6 (C A6), or determining a combination of the antibodies. Determining that the individual has SD is based on the presence of the antibodies. The method provides for detection of early SD. Kits for antibody detection containing the antigens to which the antibodies of SD patients are directed are also provided.

    摘要翻译： 提供了用于确定个体是否具有索格伦氏病（SD）的方法和组合物。 该方法需要在个体的生物样品中确定针对唾液腺蛋白1（SP-1），腮腺分泌蛋白（PSP），碳酸酐酶6（C A6）的抗体的存在，或确定抗体的组合。 确定个体具有SD是基于抗体的存在。 该方法提供早期SD的检测。 还提供了包含SD患者抗体所针对的抗原的抗体检测试剂盒。

    Method of diagnosing Sjogren's disease
    5.
    发明授权
    Method of diagnosing Sjogren's disease 有权
    诊断干燥病的方法

    公开(公告)号：US08765387B2

    公开(公告)日：2014-07-01

    申请号：US13574265

    申请日：2011-01-21

    申请人： Julian L. Ambrus ,  Long Shen

    发明人： Julian L. Ambrus ,  Long Shen

    IPC分类号： G01N33/53

    CPC分类号： G01N33/6893 ,  G01N33/564 ,  G01N2333/47 ,  G01N2333/988 ,  G01N2800/04 ,  G01N2800/101

    摘要： Provided are methods and compositions for determining whether an individual has Sjögren's disease (SD). The method entails determining in a biological sample from the individual the presence of antibodies directed to salivary gland protein 1 (SP-I), parotid secretory protein (PSP), carbonic anhydrase 6 (C A6), or determining a combination of the antibodies. Determining that the individual has SD is based on the presence of the antibodies. The method provides for detection of early SD. Kits for antibody detection containing the antigens to which the antibodies of SD patients are directed are also provided.

    摘要翻译： 提供了用于确定个体是否具有索格伦氏病（SD）的方法和组合物。 该方法需要在个体的生物样品中确定针对唾液腺蛋白1（SP-1），腮腺分泌蛋白（PSP），碳酸酐酶6（C A6）的抗体的存在，或确定抗体的组合。 确定个体具有SD是基于抗体的存在。 该方法提供早期SD的检测。 还提供了包含SD患者抗体所针对的抗原的抗体检测试剂盒。