Simple and green method for preparation of acid modified cyclodextrin

    公开(公告)号:US20230235095A1

    公开(公告)日:2023-07-27

    申请号:US18122181

    申请日:2023-03-16

    CPC classification number: C08B37/0012 B01J27/1806

    Abstract: The present disclosure discloses a simple preparation method of green acid-modified cyclodextrin, belonging to the field of cyclodextrin modification. Cyclodextrin and succinic acid are mutually modified by an esterification reaction in the presence of catalyst, and acid-modified cyclodextrin with different modification degrees is obtained by controlling reaction time at a high temperature or under a microwave action. The prepared modified cyclodextrin does not involve the use of toxic and harmful reagents, the modification reaction is simple, green and controllable, and the loading capacity of the modified cyclodextrin to guest molecules is remarkably improved compared with the loading capacity of the original cyclodextrin, so that the modified cyclodextrin has great application potential in the health fields such as food, medicines, and cosmetics.

    Method for preparing branched cyclodextrin and application thereof

    公开(公告)号:US11041179B2

    公开(公告)日:2021-06-22

    申请号:US16288765

    申请日:2019-02-28

    Abstract: The disclosure herein relates to a method for preparing branched cyclodextrin and application thereof, and belongs to the technical field of synthesis of branched cyclodextrin. The method comprises the following steps: (1) dissolving maltodextrin in a phosphate buffer solution, and adding CGTase for reacting; (2) reducing enzyme activity by a physical method; and (3) adding a saccharifying enzyme to the reaction system of step (2), and performing high-temperature enzyme deactivation to obtain the branched cyclodextrin. The method has mild reaction conditions, and at the same time, cyclodextrin and unreacted substrates can be hydrolyzed via the weak coupling activity of CGTase, thereby realizing effective separation of the branched cyclodextrin.

    Agarase mutant with improved thermal stability and application thereof

    公开(公告)号:US11999981B2

    公开(公告)日:2024-06-04

    申请号:US18489954

    申请日:2023-10-19

    CPC classification number: C12N9/2468 C12N15/63 C12Y302/01081

    Abstract: Disclosed are an agarase mutant with improved thermal stability and application thereof, belonging to the fields of genetic engineering technology and enzyme engineering. The present disclosure provides an agarase mutant, which is obtained by mutating the amino acid at the 86th site, the 373rd site, the 374th site, the 496th site, the 507th site, or the 747th site of agarase with an amino acid sequence as shown in SEQ ID NO. 1. The agarase mutant provided by the present disclosure improves the thermal stability and the hydrolytic activity of the agarase. Compared with the wild type enzyme, the mutant enzyme shows excellent heat resistance and can be industrially used at a relatively high temperature, so that the utilization rate of agar raw materials and the yield of oligosaccharides from agar are improved, and the mutant enzyme has a good industrial application prospect.

    Simple and green method for preparation of acid modified cyclodextrin

    公开(公告)号:US11840584B2

    公开(公告)日:2023-12-12

    申请号:US18122181

    申请日:2023-03-16

    CPC classification number: C08B37/0012 B01J27/1806

    Abstract: The present disclosure discloses a simple preparation method of green acid-modified cyclodextrin, belonging to the field of cyclodextrin modification. Cyclodextrin and succinic acid are mutually modified by an esterification reaction in the presence of catalyst, and acid-modified cyclodextrin with different modification degrees is obtained by controlling reaction time at a high temperature or under a microwave action. The prepared modified cyclodextrin does not involve the use of toxic and harmful reagents, the modification reaction is simple, green and controllable, and the loading capacity of the modified cyclodextrin to guest molecules is remarkably improved compared with the loading capacity of the original cyclodextrin, so that the modified cyclodextrin has great application potential in the health fields such as food, medicines, and cosmetics.

    Multi-series continuous-flow magnetoelectric coupling processing system and applications thereof

    公开(公告)号:US10460869B2

    公开(公告)日:2019-10-29

    申请号:US15548052

    申请日:2016-01-21

    Abstract: The present invention discloses a multi-series continuous-flow magnetoelectric coupling processing system and applications thereof. The processing system comprises multi-series of induction units, a power supply and a feed liquid container, wherein each series of the induction unit comprises a closed iron core, a primary coil wound around one side of the closed iron core, and a secondary coil wound around the other side of the closed iron core and arranged in an induction cavity, the secondary coil comprises an insulation pipe for circulation of feed liquid, and the two ends of the insulation pipe are exposed from the induction cavity and are respectively acted as a feeding hole and a discharge hole; the power supply is in parallel connection with each primary coil in the induction unit and provides excitation voltage to each primary coil; and the feed liquid container is in series connection with the insulation pipes of the multi-series induction units to form a feed liquid circulation loop. According to the multi-series continuous-flow magnetoelectric coupling processing system and the applications thereof, operation parameters are abundant; no electrode directly contacts with the feed liquid, thereby avoiding ionic polarization, electrochemical reactions, and heavy metal contamination; besides, the multi-series continuous-flow magnetoelectric coupling processing system has advantage of high mass transfer efficiency.

    Multi-Series Continuous-Flow Magnetoelectric Coupling Processing System and Applications Thereof

    公开(公告)号:US20180033548A1

    公开(公告)日:2018-02-01

    申请号:US15548052

    申请日:2016-01-21

    Abstract: The present invention discloses a multi-series continuous-flow magnetoelectric coupling processing system and applications thereof. The processing system comprises multi-series of induction units, a power supply and a feed liquid container, wherein each series of the induction unit comprises a closed iron core, a primary coil wound around one side of the closed iron core, and a secondary coil wound around the other side of the closed iron core and arranged in an induction cavity, the secondary coil comprises an insulation pipe for circulation of feed liquid, and the two ends of the insulation pipe are exposed from the induction cavity and are respectively acted as a feeding hole and a discharge hole; the power supply is in parallel connection with each primary coil in the induction unit and provides excitation voltage to each primary coil; and the feed liquid container is in series connection with the insulation pipes of the multi-series induction units to form a feed liquid circulation loop. According to the multi-series continuous-flow magnetoelectric coupling processing system and the applications thereof, operation parameters are abundant; no electrode directly contacts with the feed liquid, thereby avoiding ionic polarization, electrochemical reactions, and heavy metal contamination; besides, the multi-series continuous-flow magnetoelectric coupling processing system has advantage of high mass transfer efficiency.

    Modified cyclodextrin/mesoporous silica for adsorbing Pb and Cd and application thereof

    公开(公告)号:US12145866B2

    公开(公告)日:2024-11-19

    申请号:US17402871

    申请日:2021-08-16

    Abstract: The disclosure provides a modified cyclodextrin/mesoporous silica for adsorbing Pb and Cd and application thereof, belonging to the technical field of adsorbent materials. By using surface modification, chloroacetic acid is used as anions, and a modified cyclodextrin is grafted onto a surface of a cyclodextrin/mesoporous silica by nucleophilic substitution to prepare the modified cyclodextrin/mesoporous silica adsorbent material. The modified cyclodextrin/mesoporous silica adsorbent material prepared in the disclosure has the advantages of simple preparation method, strong adsorbability, easy separation, good biocompatibility and the like. When the material is used as an adsorbent to adsorb heavy metal ions Pb2+ and Cd2+, maximum removal rates can reach 97.8% and 81.29% respectively. Therefore, the material has wide application prospects in removal of heavy metals in sewage and the like, thereby improving the water environment that people depend on for survival.

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