公开(公告)号：US20080213756A1

公开(公告)日：2008-09-04

申请号：US11570138

申请日：2005-06-07

Applicant: Jeffrey A. Johnson ,  Walid Heneine

Inventor： Jeffrey A. Johnson ,  Walid Heneine

IPC: C12Q1/68

CPC classification number: C12Q1/703 ,  C12Q2600/156 ,  Y10S435/975

Abstract: Disclosed are compositions including primers and probes, which are capable of interacting with the disclosed nucleic acids, such as the nucleic acids encoding the reverse transcriptase or protease of HIV as disclosed herein. Thus, provided is an oligonucleotide comprising any one of the nucleotide sequences set for in SEQ ID NOS:1-89, and 96-104. Also provided are the oligonucleotides consisting of the nucleotides as set forth in SEQ ID NOS: 1-89, and 96-104. Each of the disclosed oligonucleotides is a probe or a primer. Also provided are mixtures of primers and probes and for use in RT-PCR and primary PCR reactions disclosed herein. Provided are methods for the specific detection of several mutations in HIV. Mutations in both the reverse transcriptase and the protease of HIV can be detected using the methods described herein.

Abstract translation: 公开了包括引物和探针的组合物，其能够与所公开的核酸相互作用，例如本文公开的编码HIV逆转录酶或蛋白酶的核酸。 因此，提供了包含SEQ ID NO：1-89和96-104中设置的任何一个核苷酸序列的寡核苷酸。 还提供了由SEQ ID NO：1-89和96-104所示的核苷酸组成的寡核苷酸。 所公开的寡核苷酸中的每一个都是探针或引物。 还提供了引物和探针的混合物，并且用于本文公开的RT-PCR和初级PCR反应。 提供了特异性检测HIV突变的方法。 可以使用本文所述的方法检测逆转录酶和HIV蛋白酶的突变。

公开(公告)号：US08318428B2

公开(公告)日：2012-11-27

申请号：US13253463

申请日：2012-01-24

Applicant: Jeffrey A. Johnson ,  Walid Heneine

Inventor： Jeffrey A. Johnson ,  Walid Heneine

IPC: C12Q1/68 ,  C07H21/04

CPC classification number: C12Q1/703 ,  C12Q2600/156 ,  Y10S435/975

Abstract: Disclosed are compositions including primers and probes, which are capable of interacting with the disclosed nucleic acids, such as the nucleic acids encoding the reverse transcriptase or protease of HIV as disclosed herein. Thus, provided is an oligonucleotide comprising any one of the nucleotide sequences set for in SEQ ID NOS:1-89, and 96-104. Also provided are the oligonucleotides consisting of the nucleotides as set forth in SEQ ID NOS:1-89, and 96-104. Each of the disclosed oligonucleotides is a probe or a primer. Also provided are mixtures of primers and probes and for use in RT-PCR and primary PCR reactions disclosed herein. Provided are methods for the specific detection of several mutations in HIV. Mutations in both the reverse transcriptase and the protease of HIV can be detected using the methods described herein.

Abstract translation: 公开了包括引物和探针的组合物，其能够与所公开的核酸相互作用，例如本文公开的编码HIV逆转录酶或蛋白酶的核酸。 因此，提供了包含SEQ ID NO：1-89和96-104中设置的任何一个核苷酸序列的寡核苷酸。 还提供了由SEQ ID NO：1-89和96-104所示的核苷酸组成的寡核苷酸。 所公开的寡核苷酸中的每一个都是探针或引物。 还提供了引物和探针的混合物，并且用于本文公开的RT-PCR和初级PCR反应。 提供了特异性检测HIV突变的方法。 可以使用本文所述的方法检测逆转录酶和HIV蛋白酶的突变。

公开(公告)号：US08043809B2

公开(公告)日：2011-10-25

申请号：US11570138

申请日：2005-06-07

Applicant: Jeffrey A. Johnson ,  Walid Heneine

Inventor： Jeffrey A. Johnson ,  Walid Heneine

IPC: C12Q1/68 ,  C07H21/04

CPC classification number: C12Q1/703 ,  C12Q2600/156 ,  Y10S435/975

Abstract: Disclosed are compositions including primers and probes, which are capable of interacting with the disclosed nucleic acids, such as the nucleic acids encoding the reverse transcriptase or protease of HIV as disclosed herein. Thus, provided is an oligonucleotide comprising any one of the nucleotide sequences set for in SEQ ID NOS:1-89, and 96-104. Also provided are the oligonucleotides consisting of the nucleotides as set forth in SEQ ID NOS:1-89, and 96-104. Each of the disclosed oligonucleotides is a probe or a primer. Also provided are mixtures of primers and probes and for use in RT-PCR and primary PCR reactions disclosed herein. Provided are methods for the specific detection of several mutations in HIV. Mutations in both the reverse transcriptase and the protease of HIV can be detected using the methods described herein.

Abstract translation: 公开了包括引物和探针的组合物，其能够与所公开的核酸相互作用，例如本文公开的编码HIV逆转录酶或蛋白酶的核酸。 因此，提供了包含SEQ ID NO：1-89和96-104中设置的任何一个核苷酸序列的寡核苷酸。 还提供了由SEQ ID NO：1-89和96-104所示的核苷酸组成的寡核苷酸。 所公开的寡核苷酸中的每一个都是探针或引物。 还提供了引物和探针的混合物，并且用于本文公开的RT-PCR和初级PCR反应。 提供了特异性检测HIV突变的方法。 可以使用本文所述的方法检测逆转录酶和HIV蛋白酶的突变。

公开(公告)号：US20120135393A1

公开(公告)日：2012-05-31

申请号：US13253463

申请日：2012-01-24

Applicant: Jeffrey A. Johnson ,  Walid Heneine

Inventor： Jeffrey A. Johnson ,  Walid Heneine

IPC: C12Q1/70 ,  C07H21/04

CPC classification number: C12Q1/703 ,  C12Q2600/156 ,  Y10S435/975

Abstract: Disclosed are compositions including primers and probes, which are capable of interacting with the disclosed nucleic acids, such as the nucleic acids encoding the reverse transcriptase or protease of HIV as disclosed herein. Thus, provided is an oligonucleotide comprising any one of the nucleotide sequences set for in SEQ ID NOS:1-89, and 96-104. Also provided are the oligonucleotides consisting of the nucleotides as set forth in SEQ ID NOS:1-89, and 96-104. Each of the disclosed oligonucleotides is a probe or a primer. Also provided are mixtures of primers and probes and for use in RT-PCR and primary PCR reactions disclosed herein. Provided are methods for the specific detection of several mutations in HIV. Mutations in both the reverse transcriptase and the protease of HIV can be detected using the methods described herein.

公开(公告)号：US20070265227A1

公开(公告)日：2007-11-15

申请号：US11669547

申请日：2007-01-31

Applicant: Walid Heneine ,  Thomas Folks ,  Robert Janssen ,  Ronald Otten ,  J. Garcia-Lerma

Inventor： Walid Heneine ,  Thomas Folks ,  Robert Janssen ,  Ronald Otten ,  J. Garcia-Lerma

IPC: A61K31/52 ,  A61K31/505 ,  A61K31/675 ,  A61P31/18

CPC classification number: A61K31/675 ,  A61K9/0034 ,  A61K9/0053 ,  A61K31/513 ,  A61K31/683 ,  A61K31/7072 ,  A61K45/06 ,  A61K2300/00

Abstract: A process is provided for protecting a primate host from a self-replicating infection by an immunodeficiency retrovirus. Protection is achieved by administering to the primate host a combination of a pharmaceutically effective amount of a nucleoside reverse transcriptase inhibitor and a pharmaceutically effective amount of a nucleotide reverse transcriptase inhibitor prior to exposure to the immunodeficiency retrovirus. The administration is effective if provided in a single dose within 24 hours of the exposure. A regime of regular daily doses is also effective in providing protection against an immunodeficiency retrovirus becoming self-replicating after infecting a primate host A process for controlling retrovirus transmission within a population includes the administration to a subpopulation at high risk for contracting an immunodeficiency retroviral infection the detailed combination prior to sexual exposure to a source of immunodeficiency retrovirus so as to preclude the immunodeficiency retrovirus from becoming self-replicating in a member of the subpopulation.

Abstract translation: 提供了一种保护灵长类动物宿主免受免疫缺陷逆转录病毒的自身复制感染的过程。 在暴露于免疫缺陷型逆转录病毒之前，通过向灵长类动物宿主施用药学有效量的核苷逆转录酶抑制剂和药学有效量的核苷酸逆转录酶抑制剂的组合来实现保护。 如果在暴露后24小时内以单一剂量提供，则该药物是有效的。 定期日剂量的方案对于在感染灵长类动物宿主之后提供免疫缺陷型逆转录病毒变得自我复制的保护也是有效的。用于控制群体内的逆转录病毒传播的方法包括向接种免疫缺陷型逆转录病毒感染的高风险的亚群施用 性暴露于免疫缺陷型逆转录病毒来源之前的详细组合，以排除免疫缺陷逆转录病毒在亚群中成为自我复制。

公开(公告)号：US20100317034A1

公开(公告)日：2010-12-16

申请号：US12829125

申请日：2010-07-01

Applicant: William M. Switzer ,  Walid Heneine ,  Thomas M. Folks ,  Nathan D. Wolfe ,  Donald S. Burke ,  Eitel Mpoudi Ngole

Inventor： William M. Switzer ,  Walid Heneine ,  Thomas M. Folks ,  Nathan D. Wolfe ,  Donald S. Burke ,  Eitel Mpoudi Ngole

IPC: G01N33/53 ,  C07K14/005 ,  C07K16/08

CPC classification number: C12Q1/701 ,  A61K2039/5256 ,  C07K14/005 ,  C12N15/86 ,  C12N2740/14021 ,  C12N2740/14022 ,  C12N2740/14043 ,  C12Q1/70

Abstract: Disclosed are compositions and methods related to the isolation and identification of the primate T-lymphotropic viruses, HTLV-3 and HTLV-4. The diversity of HTLVs was investigated among central Africans reporting contact with NHP blood and body fluids through hunting, butchering, and keeping primate pets. Herein it is shown that this population is infected with a variety of HTLVs, including two retroviruses; HTLV-4 is the first member of a novel phylogenetic lineage that is distinct from all known HTLVs and STLVs; HTLV-3 falls within the genetic diversity of STLV-3, a group that has not previously been seen in humans. The present disclosure also relates to vectors and vaccines for use in humans against infection and disease. The disclosure further relates to a variety of bioassays and kits for the detection and diagnosis of infection with and diseases caused by HTLV-3 and HTLV-4 and related viruses.

Abstract translation: 公开了与灵长动物T淋巴细胞病毒HTLV-3和HTLV-4的分离和鉴定有关的组合物和方法。 通过狩猎，屠宰和饲养灵长类宠物报告与NHP血液和体液接触的中非非洲人之间调查了HTLV的多样性。 在本文中，显示该群体感染了多种HTLV，包括两种逆转录病毒; HTLV-4是与所有已知的HTLV和STLV不同的新型系统发育谱系的第一个成员; HTLV-3属于STLV-3的遗传多样性，STLV-3是人类以前未见过的一种。 本公开还涉及用于人类抵抗感染和疾病的载体和疫苗。 本公开还涉及用于检测和诊断由HTLV-3和HTLV-4及相关病毒引起的感染和疾病的各种生物测定和试剂盒。

公开(公告)号：US07794998B2

公开(公告)日：2010-09-14

申请号：US11678596

申请日：2007-02-24

Applicant: William M. Switzer ,  Walid Heneine ,  Thomas M. Folks ,  Nathan D. Wolfe ,  Donald S. Burke ,  Eitel Mpoudi Ngole

Inventor： William M. Switzer ,  Walid Heneine ,  Thomas M. Folks ,  Nathan D. Wolfe ,  Donald S. Burke ,  Eitel Mpoudi Ngole

IPC: C12N15/09 ,  C12N7/00 ,  A61K39/00

CPC classification number: C12Q1/701 ,  A61K2039/5256 ,  C07K14/005 ,  C12N15/86 ,  C12N2740/14021 ,  C12N2740/14022 ,  C12N2740/14043 ,  C12Q1/70

Abstract: Disclosed are compositions and methods related to the isolation and identification of the primate T-lymphotropic viruses, HTLV-3 and HTLV-4. The diversity of HTLVs was investigated among central Africans reporting contact with NHP blood and body fluids through hunting, butchering, and keeping primate pets. Herein it is shown that this population is infected with a variety of HTLVs, including two retroviruses; HTLV-4 is the first member of a novel phylogenetic lineage that is distinct from all known HTLVs and STLVs; HTLV-3 falls within the genetic diversity of STLV-3, a group that has not previously been seen in humans. The present disclosure also relates to vectors and vaccines for use in humans against infection and disease. The disclosure further relates to a variety of bioassays and kits for the detection and diagnosis of infection with and diseases caused by HTLV-3 and HTLV-4 and related viruses.

Abstract translation: 公开了与灵长动物T淋巴细胞病毒HTLV-3和HTLV-4的分离和鉴定有关的组合物和方法。 通过狩猎，屠宰和饲养灵长类宠物报告与NHP血液和体液接触的中非非洲人之间调查了HTLV的多样性。 在本文中，显示该群体感染了多种HTLV，包括两种逆转录病毒; HTLV-4是与所有已知的HTLV和STLV不同的新型系统发育谱系的第一个成员; HTLV-3属于STLV-3的遗传多样性，STLV-3是人类以前未见过的一种。 本公开还涉及用于人类抵抗感染和疾病的载体和疫苗。 本公开还涉及用于检测和诊断由HTLV-3和HTLV-4及相关病毒引起的感染和疾病的各种生物测定和试剂盒。

公开(公告)号：US06566102B1

公开(公告)日：2003-05-20

申请号：US09743954

申请日：2001-01-12

Applicant: William M. Switzer ,  Walid Heneine ,  Shanmugam Vedapuri

Inventor： William M. Switzer ,  Walid Heneine ,  Shanmugam Vedapuri

IPC: C12P1934

CPC classification number: C12Q1/6888 ,  C12Q1/702 ,  C12Q2600/158

Abstract: Compositions, methods and diagnostic devices for monitoring graft integrity in xenotransplantation and for detecting infectious agents transmitted by the xenograft are described. In particular, the compositions, methods and devices are useful for determining porcine xenograft integrity and persistence and can detect the presence of PERV (porcine endogenous retrovirus) in a biological sample. The compositions, methods and devices are useful for determining or monitoring graft survival and rejection in recipients of xenografts and are useful for detecting the presence of pig cell and PERV infection in a xenotransplant recipient or donor. In addition, the compositions, methods and devices are useful for screening therapeutic products to be administered to humans to ensure that the products are free of pig cells, and thus free of PERV contamination, prior to administration.

Abstract translation: 描述了用于监测异种移植中的移植物完整性并检测由异种移植物传播的感染因子的组合物，方法和诊断装置。 特别地，组合物，方法和装置可用于确定猪异种移植物完整性和持久性，并且可以检测生物样品中PERV（猪内源性逆转录病毒）的存在。 组合物，方法和装置可用于确定或监测异种移植物接受者的移植物存活和排异，并且可用于检测异种移植受体或供体中猪细胞和PERV感染的存在。 此外，组合物，方法和装置可用于筛选待给予人的治疗产品，以确保产品在施用之前不含猪细胞，因此不含PERV污染。

公开(公告)号：US20080292657A1

公开(公告)日：2008-11-27

申请号：US11678596

申请日：2007-02-24

Applicant: William M. Switzer ,  Walid Heneine ,  Thomas M. Folks ,  Nathan D. Wolfe ,  Donald S. Burke ,  Eitel Mpoudi-Ngole

Inventor： William M. Switzer ,  Walid Heneine ,  Thomas M. Folks ,  Nathan D. Wolfe ,  Donald S. Burke ,  Eitel Mpoudi-Ngole

IPC: A61K39/00 ,  C12N15/11 ,  C12N7/00 ,  A61P37/00 ,  C12Q1/70 ,  C12N15/00

CPC classification number: C12Q1/701 ,  A61K2039/5256 ,  C07K14/005 ,  C12N15/86 ,  C12N2740/14021 ,  C12N2740/14022 ,  C12N2740/14043 ,  C12Q1/70

Abstract: Disclosed are compositions and methods related to the isolation and identification of the primate T-lymphotropic viruses, HTLV-3 and HTLV-4. The diversity of HTLVs was investigated among central Africans reporting contact with NHP blood and body fluids through hunting, butchering, and keeping primate pets. Herein it is shown that this population is infected with a variety of HTLVs, including two retroviruses; HTLV-4 is the first member of a novel phylogenetic lineage that is distinct from all known HTLVs and STLVs; HTLV-3 falls within the genetic diversity of STLV-3, a group that has not previously been seen in humans. The present disclosure also relates to vectors and vaccines for use in humans against infection and disease. The disclosure further relates to a variety of bioassays and kits for the detection and diagnosis of infection with and diseases caused by HTLV-3 and HTLV-4 and related viruses.

Abstract translation: 公开了与灵长动物T淋巴细胞病毒HTLV-3和HTLV-4的分离和鉴定有关的组合物和方法。 通过狩猎，屠宰和饲养灵长类宠物报告与NHP血液和体液接触的中非非洲人之间调查了HTLV的多样性。 在本文中，显示该群体感染了多种HTLV，包括两种逆转录病毒; HTLV-4是与所有已知的HTLV和STLV不同的新型系统发育谱系的第一个成员; HTLV-3属于STLV-3的遗传多样性，STLV-3是人类以前未见过的一种。 本公开还涉及用于人类抵抗感染和疾病的载体和疫苗。 本公开还涉及用于检测和诊断由HTLV-3和HTLV-4及相关病毒引起的感染和疾病的各种生物测定和试剂盒。

公开(公告)号：US06800475B1

公开(公告)日：2004-10-05

申请号：US10018627

申请日：2001-12-14

Applicant: William M. Switzer ,  Walid Heneine ,  Paul A. Sandstrom ,  Thomas M. Folks

Inventor： William M. Switzer ,  Walid Heneine ,  Paul A. Sandstrom ,  Thomas M. Folks

IPC: C12N700

CPC classification number: C07K14/005 ,  A61K35/00 ,  A61K48/00 ,  A61K2039/5256 ,  C12N7/00 ,  C12N15/86 ,  C12N2740/17021 ,  C12N2740/17022 ,  C12N2740/17043

Abstract: The present invention comprises compositions and methods comprising a spumavirus isolated from a human. More specifically, the spumavirus of the present invention was isolated from a human who had exposure to nonhuman primates. Importantly, the methods and compositions of the present invention comprising the spumavirus and including antibodies to the spumavirus, can be used to detect the presence of spumavirus or antibodies in body fluids, for pathogenicity studies of related viruses, and as a vector for gene therapies. The present invention can also be used for treatment of conditions in humans due to the presence of rapidly dividing cells and for recombinant live virus vaccination.

Abstract translation: 本发明包括组合物和方法，其包含从人分离的真菌病毒。 更具体地，将本发明的真菌病毒与暴露于非人灵长类动物的人分离。 重要的是，包含该真菌病毒并包括针对该病毒的抗体的本发明的方法和组合物可用于检测体液中的真菌病毒或抗体的存在，相关病毒的致病性研究以及用于基因治疗的载体。 本发明还可用于治疗由于快速分裂的细胞的存在和用于重组活病毒疫苗接种的人的病症。