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    HYBRIDOMA CELL STRAIN SECRETING NIFURSOLOL RESIDUE MARKER MONOCLONAL ANTIBODY
    3.
    发明申请
    HYBRIDOMA CELL STRAIN SECRETING NIFURSOLOL RESIDUE MARKER MONOCLONAL ANTIBODY 审中-公开

    公开(公告)号：US20200347119A1

    公开(公告)日：2020-11-05

    申请号：US16934709

    申请日：2020-07-21

    申请人： JIANGNAN UNIVERSITY

    发明人： Hua KUANG ,  Chuanlai XU ,  Aihong WU ,  Liguang XU ,  Wei MA ,  Liqiang LIU ,  Xiaoling WU ,  Shanshan SONG ,  Yongming HU

    IPC分类号： C07K16/06 ,  C07K16/12 ,  C07K16/44 ,  G01N33/12

    摘要： A hybridoma cell strain secreting a nifursol residue marker monoclonal antibody prepared in the following way: BALB/c mice are subjected to the first immunization with a complete Freund's adjuvant, subjected to booster immunization with an incomplete Freund's adjuvant for four times, and subjected to rush immunization once with nifursol residue marker complete antigen without a Freund's adjuvant so that the BALB/c mice are immunized; the spleen cells of the immunized mice with high titer and low IC50 were fused with mouse myeloma cells by a PEG method, and the fused cells are screened through indirect competitive ELISA and subcloned three times. The monoclonal antibody secreted by this cell strain has good specificity and detection sensitivity (IC50 value of 2 μg/L) to the nifursol residue marker and can be used for residue detection of the nifursol residual marker in food.

    Lincosamides Universal Monoclonal Antibody Hybridoma Cell Strain and Application Thereof
    4.
    发明申请
    Lincosamides Universal Monoclonal Antibody Hybridoma Cell Strain and Application Thereof 审中-公开

    公开(公告)号：US20200095337A1

    公开(公告)日：2020-03-26

    申请号：US16699048

    申请日：2019-11-28

    申请人： Jiangnan University

    发明人： Hua KUANG ,  Lingling GUO ,  Chuanlai XU ,  Liguang XU ,  Wei MA ,  Liqiang LIU ,  Shanshan SONG ,  Xiaoling WU

    IPC分类号： C07K16/44 ,  G01N33/94

    摘要： The present disclosure discloses a lincosamides universal monoclonal antibody hybridoma cell strain and application thereof, and belongs to the technical field of food safety immunological detection. According to the present disclosure, a clindamycin chlorine-substituted derivative is used as a hapten, the hapten is coupled with bovine serum albumin (BSA) by an activated ester method to obtain an immunizing antigen, and after being uniformly mixed with a Freund's adjuvant, the immunizing antigen is subcutaneously injected to immunize BALB/c mice; clindamycin is coupled with ovalbumin (OVA) by a carbonyl diimidazole (CDI) method to be used as a coating antigen used for detecting mouse serums and a cell supernatant. The spleen cells of the immunized mice are fused with mouse myeloma cells by a PEG method, and screened by indirect ELISA and indirect competitive ELISA and subcloned three times to obtain a population-selective hybridoma cell strain. The cell strain provided by the present disclosure has relatively good inhibition on clindamycin, lincomycin and pirlimycin, and can meet the demand for lincosamides multi-residue immunoassay products on the market.

    HYBRIDOMA CELL STRAIN SECRETING NIFURSOLOL RESIDUE MARKER MONOCLONAL ANTIBODY
    7.
    发明申请
    HYBRIDOMA CELL STRAIN SECRETING NIFURSOLOL RESIDUE MARKER MONOCLONAL ANTIBODY 审中-公开

    公开(公告)号：US20200048329A1

    公开(公告)日：2020-02-13

    申请号：US16221719

    申请日：2018-12-17

    申请人： JIANGNAN UNIVERSITY

    发明人： Hua KUANG ,  Chuanlai XU ,  Aihong WU ,  Liguang XU ,  Wei MA ,  Liqiang LIU ,  Xiaoling WU ,  Shanshan SONG ,  Yongming HU

    IPC分类号： C07K16/06 ,  G01N33/12 ,  C07K16/12 ,  C07K16/44

    摘要： A hybridoma cell strain secreting a nifursolol residue marker monoclonal antibody with CGMCC No. 14698 is prepared in the following way: BALB/c mice are subjected to the first immunization with a complete Freund's adjuvant, subjected to booster immunization with an incomplete Freund's adjuvant for four times, and subjected to rush immunization once with nifursolol residue marker complete antigen without a Freund's adjuvant so that the BALB/c mice are immunized; the spleen cells of the immunized mice with high titer and low IC50 were fused with mouse myeloma cells by a PEG method, and the fused cells are screened through indirect competitive ELISA and subcloned three times. The monoclonal antibody secreted by this cell strain has good specificity and detection sensitivity (IC50 value of 2 μg/L) to the nifursolol residue marker and can be used for residue detection of the nifursolol residual marker in food.