公开(公告)号：US20230167196A1

公开(公告)日：2023-06-01

申请号：US17779989

申请日：2021-11-09

申请人： JIANGNAN UNIVERSITY

发明人： Chuanlai XU ,  Jingjing YAO ,  Hua KUANG ,  Liguang XU ,  Maozhong SUN ,  Xiaoling WU ,  Liqiang LIU ,  Wei MA ,  Jianping ZHU ,  Changlong HAO ,  Shanshan SONG ,  Yongming HU ,  Aihong WU ,  Lingling GUO ,  Xinxin XU

IPC分类号： C07K16/44 ,  G01N33/94

CPC分类号： C07K16/44 ,  G01N33/9486 ,  C07K2317/92 ,  G01N2470/10

摘要： The invention discloses an anti-phenacetin monoclonal antibody hybridoma cell strain AD, a preparation method and application thereof, and relates to the technical field of food safety immunodetection. The monoclonal antibody hybridoma cell strain is named monoclonal cell strain AD and the number CGMCC19681. The Phe-BA obtained by the hydrolysis of the reaction product of the phenacetin metabolite acetaminophen and ethyl 4-bromobutyrate is used as the hapten, and the hapten is coupled with the carrier protein to prepare the immunogen Phe-BA-BSA. After the mice were immunized with the immunogen Phe-BA-BSA, they were fused with myeloma cells by PEG method, screened by indirect competitive enzyme-linked immunosorbent assay and subcloned five times to obtain hybridoma cell lines. The monoclonal antibody secreted by the cell line can be made into a phenacetin detection kit, which has good affinity and detection sensitivity for phenacetin, and can be used for immunodetection of phenacetin residues in food.

公开(公告)号：US20220010029A1

公开(公告)日：2022-01-13

申请号：US17368108

申请日：2021-07-06

申请人： JIANGNAN UNIVERSITY

发明人： Chuanlai XU ,  JIE LIU ,  HUA KUANG ,  Liguang XU ,  Maozhong SUN ,  Liqiang LIU ,  Xiaoling WU ,  Changlong HAO ,  Shanshan SONG ,  Yongming HU ,  Qiankun ZHENG

IPC分类号： C07K16/44 ,  G01N33/53

摘要： A hybridoma cell strain secreting anti-dinitolmide monoclonal antibodies deposited at China General Microbiological Culture Collection Center (CGMCC) at No. 1 West Beichen Road, Chaoyang District, Beijing 100101, China on Nov. 28, 2019, with deposit number of CGMCC No. 19165. It is classified as a monoclonal cell strain. The monoclonal antibody secreted by the hybridoma cell strain DAS3H10 has a good affinity and high sensitivity to dinitolmide. Because of IC50 to dinitolmide up to 9.01 ng/mL, the monoclonal antibody could be used to prepare dinitolmide immunoassay kit and colloidal gold test strip and provides a powerful detection method and means for the detection of dinitolmide in animal-derived foods.