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    METHOD FOR IMAGING CELL USING FLUORESCENCE-LABELED SUGAR DERIVATIVE HAVING COUMARIN DERIVATIVE BOUND THERETO, AND IMAGING AGENT
    1.
    发明申请
    METHOD FOR IMAGING CELL USING FLUORESCENCE-LABELED SUGAR DERIVATIVE HAVING COUMARIN DERIVATIVE BOUND THERETO, AND IMAGING AGENT 审中-公开
    使用具有香豆素衍生化合物的荧光标记糖衍生物成像细胞和成像剂的方法

    公开(公告)号：US20150369797A1

    公开(公告)日：2015-12-24

    申请号：US14431523

    申请日：2013-10-01

    申请人： Hirosaki University

    发明人： Katsuya YAMADA ,  Tadashi TESHIMA ,  Toshihiro YAMAMOTO

    IPC分类号： G01N33/50 ,  C07H3/02

    CPC分类号： G01N33/5091 ,  A61K49/0039 ,  A61K49/0052 ,  C07H3/02 ,  C07H13/10 ,  G01N33/533 ,  G01N33/574 ,  G01N33/582 ,  G01N2400/00 ,  G01N2800/7028

    摘要： The present invention has an object of providing a sugar derivative emitting blue fluorescence color which can be used for imaging of cells or intracellular molecules and a method for imaging cells using the derivative. Further, the present invention has an object of providing a method for detecting cancer cells at high accuracy by imaging, and an imaging agent used for this method. The present invention provides a fluorescently labeled sugar derivative having 3-carboxy-6,8-difluoro-7-hydroxycoumarin or 3-carboxymethyl-6,8-difluoro-7-hydroxy-4-methylcoumarin as a fluorescent molecular group in its molecule, and a cell imaging agent and an imaging method using the derivative. Further, the present invention provides an imaging agent and an imaging method for cancer cells using an L-glucose derivative having the above-described fluorescent molecular group in its molecule.

    摘要翻译： 本发明的目的是提供一种可用于细胞或细胞内分子成像的蓝色荧光染料衍生物和使用该衍生物成像细胞的方法。 此外，本发明的目的是提供一种通过成像以高精度检测癌细胞的方法和用于该方法的成像剂。 本发明提供具有3-羧基-6,8-二氟-7-羟基香豆素或3-羧甲基-6,8-二氟-7-羟基-4-甲基香豆素的荧光标记糖衍生物作为其分子中的荧光分子， 和细胞成像剂和使用该衍生物的成像方法。 此外，本发明提供了在其分子中使用具有上述荧光分子基团的L-葡萄糖衍生物的癌细胞的成像剂和成像方法。

    CANCER CELL DETECTION METHOD USING LIVING BODY-DERIVED CELLS OF BIOLOGICAL ORIGIN
    3.
    发明申请
    CANCER CELL DETECTION METHOD USING LIVING BODY-DERIVED CELLS OF BIOLOGICAL ORIGIN 审中-公开

    公开(公告)号：US20170315129A1

    公开(公告)日：2017-11-02

    申请号：US15513259

    申请日：2015-09-24

    申请人： Hirosaki University

    发明人： Katsuya YAMADA ,  Ayako SASAKI ,  Kouki ONO ,  Kiyoshi TONE

    IPC分类号： G01N33/58

    摘要： The purpose of the present invention is to provide a novel cancer cell detection method that uses living body-derived cells and that can be used even in cytodiagnosis. In particular, the purpose of the present invention is to provide a novel cancer cell detection method that makes it possible to perform imaging of cells in a living state and a dual detection method for cancer cells in which the aforementioned method is combined with a pre-existing dyeing method for cytodiagnosis. Provided is a cancer cell detection method that uses living body-derived cells and that includes: incubating living cells included in a sample taken from a person together with a fluorescently-labeled L-glucose derivative and detecting the fluorescently-labeled L-glucose derivative that is taken up into the cells; and detecting fluorescence emitted by the L-glucose derivative that is present within the cells while the cells are attached to a thin glass or plastic plate. Also provided is a dual detection method for cancer cells in which the cancer cell detection method that uses living body-derived cells is combined with a dyeing method using cells that are fixed using an alcohol or the like.

    L-glucose derivatives having fluorescent chromophore
    4.
    发明申请
    L-glucose derivatives having fluorescent chromophore 审中-公开
    具有荧光发色团的L-葡萄糖衍生物

    公开(公告)号：US20150212094A1

    公开(公告)日：2015-07-30

    申请号：US14614854

    申请日：2015-02-05

    申请人： HIROSAKI UNIVERSITY

    发明人： Katsuya YAMADA ,  Tadashi TESHIMA ,  Toshihiro YAMAMOTO

    IPC分类号： G01N33/58 ,  C09B57/00 ,  C09B51/00

    CPC分类号： G01N33/582 ,  C09B51/00 ,  C09B57/00 ,  C12Q1/02 ,  C12Q1/54 ,  G01N33/5005 ,  G01N33/533

    摘要： An object of the present invention is to provide a method for accurately evaluating the specific incorporation of D-glucose into cells. The present invention as a means for achieving the object is characterized by comprising contacting a D-glucose derivative that has a fluorescent chromophore in the molecule and is specifically incorporated into cells and an L-glucose derivative that has a fluorescent chromophore in the molecule with different cells in the same cell strain to be evaluated, respectively, comparing the fluorescence emitted by the D-glucose derivative that has a fluorescent chromophore in the molecule and is specifically incorporated into cells with the fluorescence emitted by the L-glucose derivative that has a fluorescent chromophore in the molecule, and evaluating the specific incorporation of D-glucose into cells relative to L-glucose by taking the difference between the two kinds of fluorescence intensities.

    摘要翻译： 本发明的目的是提供一种用于精确评估D-葡萄糖在细胞中的特异性掺入的方法。 作为实现该目的的手段的本发明的特征在于，使分子中具有荧光发色团的D-葡萄糖衍生物与细胞特异性结合，并且在分子中具有荧光发色团的L-葡萄糖衍生物不同 分别将待分析的具有荧光发色团的D-葡萄糖衍生物发射的荧光发射到具有荧光的L-葡萄糖衍生物发射的荧光的细胞中进行评价的细胞， 并且通过取两种荧光强度之间的差异来评估D-葡萄糖相对于L-葡萄糖的细胞特异性掺入细胞。