公开(公告)号：US11898270B2

公开(公告)日：2024-02-13

申请号：US16628481

申请日：2017-08-21

申请人： HUAZHONG AGRICULTURAL UNIVERSITY

发明人： Shuhong Zhao ,  Shengsong Xie ,  Changzhi Zhao ,  Xinyun Li ,  Xiangdong Liu ,  Xiaosong Han ,  Gaojuan Yang ,  Yang Gao ,  Yilong Chen ,  Xiaoyong Du ,  Yiliang Miao ,  Yunlong Ma ,  Xiaolei Liu

IPC分类号： C12N15/90 ,  C40B40/02 ,  C12N9/22 ,  C12N15/113 ,  C12N15/85 ,  C12Q1/6869 ,  C40B40/06 ,  C40B50/06

CPC分类号： C40B40/02 ,  C12N9/22 ,  C12N15/113 ,  C12N15/85 ,  C12N15/907 ,  C12Q1/6869 ,  C40B40/06 ,  C40B50/06 ,  C12N2810/10

摘要： Provided is a pig genome-wide specific sgRNA library, a preparation method therefor, and an application thereof. The sgRNA is targeted at a pig genome-wide protein-coding gene, lincRNA and/or miRNA. Specifically, an sgRNA construct has the following structure: AL-N20-AR, wherein AL is the left homology arm sequence located at the upstream of the coding sequence of a pig specific SgRNA, N20 is the coding sequence of the pig specific SgRNA, and AR is a right homology arm sequence located at the downstream of the coding sequence of the pig specific sgRNA. The sgRNA library can be used for screening functional genes of a pig or for preparing a kit.