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公开(公告)号：US11732234B1
公开(公告)日：2023-08-22
申请号：US17984238
申请日：2022-11-10
申请人： Jiangxi Agricultural University , Guangzhou Institute of Geochemistry, Chinese Academy of Sciences , Huazhong Agricultural University
发明人： Qin Zhang , Dong Liu , Wenfeng Tan , Guanjie Jiang , Taihui Zheng , Yupeng Yan
摘要： The present application discloses the use of diatom in rice planting and a cultivation method of rice in symbiosis with diatom and relates to the technical field of rice planting. The use of diatom in rice planting is achieved by adding diatom to an irrigated paddy field and performing co-cultivation during rice planting. Co-cultivation of diatom and rice can effectively achieve the beneficial effects of increased yield/silicon, thicker straw, and increased lodging resistance in rice, and can reduce soil degradation, reduce the risk of heavy metal pollution and increase soil fertility.
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公开(公告)号：US11685931B2
公开(公告)日：2023-06-27
申请号：US17554881
申请日：2021-12-17
发明人： Zexia Gao , Chunhong Nie , Shiming Wan , Yulong Chen , Dejie Zhu
IPC分类号： C12N15/85 , A01K67/027 , C12N9/22 , C12N15/11
CPC分类号： C12N15/8509 , A01K67/0275 , C12N9/22 , C12N15/11 , A01K2217/077 , A01K2227/40 , C12N2310/20 , C12N2800/80
摘要： Application of a fragment of an isolated nucleotide sequence in the construction of zebrafish without intermuscular bones. The nucleotide sequence is shown in SEQ ID NO:1. Gene mutation is performed by taking SEQ ID NO:1 as a target gene; the mutant F0 embryos are selected and cultured to adult fish; F0 mutant is hybridized with wild type zebrafish to generate an F1 embryos; sense mutant heterozygotes F1 is screened out and cultured to adult fish; and then F1 heterozygote self-crosses to generate F2 generation of three gene types, including homozygote, heterozygote, and wild type. Zebrafish without intermuscular bones is obtained by using a gene mutation method, which provided a basis for subsequent research on a molecular formation mechanism of fish intermuscular bones and the cultivation of economic fishes without intermuscular bone and possessed a basic research value and an application value in other economic aquaculture fish species.
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公开(公告)号：US11576385B2
公开(公告)日：2023-02-14
申请号：US16856583
申请日：2020-04-23
发明人： Daohong Jiang , Binnian Tian , Jiatao Xie , Yanping Fu , Jiasen Cheng , Tao Chen
IPC分类号： A01N63/30
摘要： The present invention provides a method for increasing wheat yield and preventing wheat diseases and use thereof, and relates to the technical field of crop cultivation. In the method, wheat seeds are primed by a fermentation liquid of S. sclerotiorum, and the concentration of the fermentation liquid is 1.4×105 cfu/mL or more. According to the present invention, the wheat seeds are primed by using S. sclerotiorum, and S. sclerotiorum can endogenously grow in wheat plants to induce expression of genes related to disease resistance and development of wheat, thereby achieving the objective of controlling diseases such as wheat Fusarium head blight and improving wheat yield.
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公开(公告)号：US11339401B2
公开(公告)日：2022-05-24
申请号：US16803915
申请日：2020-02-27
发明人： Xuelu Wang , Shiyong Sun
IPC分类号： C12N15/82 , C07K14/415
摘要： A method for controlling the building of architecture of gramineous crops and application thereof. The gene CYC U4;1 (Os10g41430) has a specific expression in a rice pulvinus (which is believed to have a specific expression in other gramineous crops), with a CDS sequence as shown in SEQ ID NO: 2, and an encoded amino acid sequence as shown in SEQ ID NO: 3. Also a promoter sequence of the gene, with the sequence as shown in SEQ ID NO: 1. Transgenic lines obtained by cloning a promoter and full-length CDS of CYC U4;1 to pCAMBIA1301 and transferring this into rice Nipponbare are all characterized by having smaller leaf-stem angles than those of wild rice, accordingly, the expression level of the gene CYC U4;1 can be increased or decreased with genetic engineering technology to control the plant architecture development, thereby improving the plant architecture and increasing the density of germplasm.
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公开(公告)号：US20220087209A1
公开(公告)日：2022-03-24
申请号：US17418694
申请日：2019-11-07
发明人： Liguo YANG , Guohua HUA , Kaifeng NIU , Hongxue SHEN , Lifeng SHI , Dianshuang ZHANG
摘要： The invention discloses a cattle shed, comprising a cattle bedding, a feeding channel, a fixed roof, a movable roof, a feeding trough, a peripheral rail fence, and a rail fence gate, wherein the cattle bedding is provided with no dividers and needs no hardening and daily manure cleaning, which is merged with playground and enclosed. The feeding channel is hardened and higher than the cattle bedding, the fixed roof and the movable roof cover the cattle bedding and the feeding trough, the peripheral rail fence and the rail fence gate surround the cattle bedding and the feeding trough, and the fixed roof and the movable roof are arranged in a slope mode, and the movable roof can be opened to allow sunlight to enter and exchange air to form convection. The cattle shed of the present invention can reduce the construction cost, open the movable roof, effectively utilize the sunlight and the air convection, ensure a complete rain-sewage separation, and realize zero discharge of manure and sewage.
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公开(公告)号：US20210214743A1
公开(公告)日：2021-07-15
申请号：US17147105
申请日：2021-01-12
发明人： Zhibing LAI , Hongze WANG , Jiabao HOU
IPC分类号： C12N15/82
摘要： The present disclosure is related to plant breeding and methods of identifying and selecting plants with disease resistance. Provided are methods to identify novel genes that encode proteins providing plant disease resistance and uses thereof. These disease resistant genes are useful in the production of resistant plants through breeding, transgenic modification, or genome editing.
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7.发明申请公开(公告)号：US20200216853A1
公开(公告)日：2020-07-09
申请号：US16746924
申请日：2020-01-19
发明人： YUBING HE
IPC分类号： C12N15/82
摘要： The invention relates to a plant genetic engineering field, and more particularly to a method for creating directed gene mutated non-transgenic plants. The method including performing a transgenic method onto directed gene mutated plants by introducing exogenous nucleic acid molecules; wherein the transgenic method includes introducing constructs into the directed gene mutated plants, each of the constructs contains a first nucleic acid molecule and a second nucleic acid molecule, wherein the first nucleic acid molecule serves as a gene editing element, and the second nucleic acid molecule serves as a lethal or stop development element, and can be used in a plant gene editing system such as CRISPR/CAS9. It can actively and automatically eliminate plant transgenic fragments, leaving enough time for gene editing elements to perform directed gene editing before removing transgenic fragments, providing a simple and effective method for gene editing without transgenic plants.
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公开(公告)号：US20200030385A1
公开(公告)日：2020-01-30
申请号：US16173153
申请日：2018-10-29
发明人： Xi Huang , Xiaoyun Li , Meihu Ma , Zhaoxia Cai , Xing Fu
摘要： A water resistant enhanced wound healing film includes components by mass volume concentration of: 5-10 mg/L of ultra-fine eggshell powder, 50-500 mg/L of ultramicroshell membrane powder or shell membrane soluble protein, 5-30 mg/L of lysozyme, 5-10 mg/L of chitosan, 0.05-5 mg/L of glycerin, and 0.1-5 mg/L of polymer polysaccharide. Also, a preparation method of the water resistant enhanced wound healing film is disclosed. The whole raw materials of the present invention are non-toxic and have good biocompatibility; the raw materials include eggshell, shell membrane and soluble mixed protein thereof, and lysozyme in residual egg white. In the present invention, various components of the eggshell waste are sufficiently used. The wound healing film has good water resistance, mechanical properties, antibacterial activity and protein absorption capacity, and low degradation rate. The method of the present invention has simple process, strong practicability, extremely low production cost, favorable resource recycling and low energy consumption.
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公开(公告)号：US20200000761A1
公开(公告)日：2020-01-02
申请号：US16566886
申请日：2019-09-11
发明人： Xiaoyun Xu , Ting Wu , Weiwei Cao , Siyi Pan , Hui Li
IPC分类号： A61K31/216
摘要： 1,4-dicaffeoylquinic acid is used as a xanthine oxidase inhibitor and in preparation of a medicament for treating gout. The present invention is the first to isolate and identify a dicaffeoylquinic acid compound from Artemisia selengensis leaves. The compound not only has an ability of inhibiting xanthine oxidase activity, but also has the function of anti-gout inflammation, which can be better used for treating gout. The dicaffeoylquinic acid is derived from natural plants and has the advantages of being safe and non-toxic, and having fewer side effects.
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公开(公告)号：US10378052B2
公开(公告)日：2019-08-13
申请号：US14990825
申请日：2016-01-08
发明人： Meizhong Luo , Yonglong Pan
IPC分类号： G01N33/48 , C12Q1/6874 , C12Q1/6806 , G16B30/00
摘要： A whole-genome sequencing method, including: 1) extracting a whole-genome DNA and constructing a BAC library; 2) constructing mixing pools; 3) extracting DNA of the mixing pools; 4) performing pair-end sequencing for the DNA from the mixing pools; 5) scanning sequences of each mixing pool to obtain a feature sequence set and a k-mer set of each mixing pool; 6) analyzing a corresponding feature sequence set and a corresponding k-mer set of each clone; 7) constructing clone contigs; 8) dividing the k-mer sets of the clones into small k-mer sets and positioning the small k-mer sets; 9) assembling the NGS sequences to yield sequence contigs; 10) positioning the sequence contigs, connecting the sequence contigs and determining directions thereof; and 11) determining relative positions and directions of the clone contigs, and connecting the sequences of clone contigs as whole stripe of chromosome sequences.
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